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Background and purpose: Gastric cancer is one of the common gastrointestinal malignancies. FKBP1A has been reported to be involved in the occurrence and development of various tumors, but the biological role and mechanism of it in gastric cancer remain unclear. This study aimed to investigate the expression level and prognostic value of FKBP1A in gastric cancer tissues, and to analyze the possible pathways and mechanisms of its regulation of gastric cancer progression. Methods: The expression of FKBP1A in gastric cancer was observed by immunohistochemistry, and its correlation with poor prognosis was analyzed by combining bioinformatics and clinicopathological parameters. Kaplan-Meier survival curve was used to analyze the effect of FKBP1A on the 5-year survival rate of patients with gastric cancer after surgery, and COX multivariate regression analysis was used to explore the independent prognostic factors affecting the 5-year survival rate of patients with gastric cancer after surgery. The diagnostic value of FKBP1A expression in patients with gastric cancer was analyzed using receiver operating characteristic (ROC) curve. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) were used to enrich and analyze the biological function of FKBP1A and the possible signal pathways involved. We constructed the MGC803 cell model transfected with lentivirus in vitro, explored the influence of FKBP1A on the glucose metabolism and malignant biological behavior of MGC803 cells, and the possible molecular mechanism involved, and established a nude mouse transplantation tumor model in vitro to verify it. Results: Immunohistochemical results showed that FKBP1A was highly expressed in gastric cancer (P＜0.01), and bioinformatics and clinical parameter analysis showed that FKBP1A was associated with poor prognosis. Kaplan-Meier survival analysis and COX regression analysis showed that the expression level of FKBP1A was negatively correlated with 5-year survival. And carcinoembryonic antigen (CEA)≥5 μg/L, carbohydrate antigen (CA) 19-9≥37 kU/L, T stage (T3-T4) and N stage (N2-N3) were independent prognostic factors affecting the 5-year survival rate of gastric cancer patients after surgery (P＜0.05). ROC analysis showed that high expression of FKBP1A had good prognostic value (P<0.01). Enrichment of GO and KEGG suggested that FKBP1A was involved in regulating glucose metabolism in gastric cancer cells. In vitro experiments showed that overexpression of FKBP1A promoted glucose metabolism and proliferation, invasion and migration of MGC803 cells, while silencing of FKBP1A did the opposite (P＜0.05). In vivo experiments showed that overexpression of FKBP1A in gastric cancer cells promoted the growth of transplanted tumor in nude mice, while silencing FKBP1A inhibited it (P＜0.05). Mechanism analysis showed that overexpression of FKBP1A upregulated the expressions of phosphatidylinositol 3-kinase (PI3K) and protein kinase B (AKT) in gastric cancer cells, while silencing FKBP1A downregulated the expressions (P＜0.05). Conclusion: FKBP1A is highly expressed in gastric cancer tissues, and is associated with poor prognosis, which may be due to the promotion of glucose metabolism and malignant biological behavior of gastric cancer cells by activating PI3K/AKT. [ABSTRACT FROM AUTHOR]

To analyze the clinicopathological features and prognosis difference of Luminal B type breast cancer patients with different subtypes. The clinicopathological and follow-up data of 359 cases of primary Luminal B type breast cancer patients who were admitted to Xuhui And Lingang Hospitals of Shanghai Sixth People's Hospital from January 2006 to January 2016 were retrospectively analyzed. According to Luminal B type breast cancer molecular subtypes, the patients were divided into the human epidermal growth receptor 2 (Her-2) negative group (266 cases) and the Her-2 positive group (93 cases). The clinicopathological features difference of the two groups were compared. Kaplan-meier survival curve was drawn to analyze the difference of 5-year progression-free survival rate and overall survival rate between the two groups, and Cox proportional regression was used to analyze the influencing factors of the prognosis of Luminal B type breast cancer patients. TNM stage III～IV stage, tumor histological grade III grade, PR negative, Ki-67 high expression and lymph node metastasis proportion in Her-2 positive group were higher than those in Her-2 negative group (P＜ 0.05). The 5-year DFS and OS survival rates in Her-2 positive group were 12.90% and 40.86%, respectively, which were lower than 24.06% and 59.77% in Her-2 negative group (Log-Rank χ2 =14.500, 12.860, P=0.000, 0.000). TNM stage III～IV stage, lymph node metastasis, Her-2 positive and PR negativewere the risk factors for poor prognosis in Luminal B type breast cancer patients ( P ＜ 0.05). There are differences in TNM stage, tumor histological grade, lymph node metastasis, Ki-67 and PR expression in different molecular subtypes of Luminal B type breast cancer patients. Meanwhile, TNM stage, lymph node metastasis, Her-2 positive and PR negative are risk factors for poor prognosis in Luminal B type breast cancer patients. [ABSTRACT FROM AUTHOR]