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BACKGROUND: Currently, electrospun nanofibers, which are biomimetic materials of natural extracellular matrix and contain a three-dimensional network of interconnected pores, have been successfully used as scaffolds for various tissue regeneration, but are still faced with the challenge of extending the biomaterials into three-dimensional structures to reproduce the physiological, chemical as well as mechanical properties of the tissue microenvironment. OBJECTIVE: To summarize the process and principles of electrostatic spinning and to explore the applications of the resulting electrospun nanofibers in tissue regeneration of skin, blood vessels, nerves, bone, cartilage and tendons/ligaments. METHODS: With “electrospinning, electrospun nanofibers, electrospun nanofiber scaffolds, tissue regeneration” as the Chinese and English search terms, Google Academic Database, PubMed, and CNKI were searched, and finally 88 articles were included for review. RESULTS AND CONCLUSION: (1) The electrospun nanofibers are a natural fibrous extracellular matrix mimetic material and contain a three-dimensional network of interconnected pores that have been successfully used as scaffolds for a variety of tissue regeneration applications. (2) Several papers have described the great potential of electrospun nanofiber scaffolds applied to the regeneration of skin, blood vessels, nerves, bones, cartilage and tendons/ ligaments, providing a solid theoretical basis for its final application in clinical disease treatment, or for its transformation into practical products to enter the market. (3) However, the current research results are mostly based on cell experimental research results in vitro, and whether it can be finally applied to human body still needs clinical verification. (4) At present, many kinds of electrospun products for various clinical needs have been commercialized in and outside China, indicating that the research field of electrospun nanofiber scaffolds for soft and hard tissue regeneration has great research value and application potential. [ABSTRACT FROM AUTHOR]

BACKGROUND: Stem cells play an important role in liver regeneration and self-repair ability, which is of great significance for clinical diagnosis and treatment of liver diseases. OBJECTIVE: To summarize the current clinical trials and basic research in this field in and outside China, review the types of stem cells used to treat liver diseases in the past, and the treatment programs of various types of stem cells for various types of liver diseases, outline the clinical application prospects of various types of stem cells in the future application of liver diseases, and provide a research basis for the treatment of clinical liver diseases. METHODS: Using “stem cells, mesenchymal stem cells, hepatic stem cell, embryonic stem cell, induced pluripotent stem cell, hematopoietic stem cell, hepatopathy, non-alcoholic fatty liver disease, liver cirrhosis, liver failure, autoimmune hepatitis, hepatocellular carcinoma” as search words, the relevant articles in PubMed, Web of Science and Wiley e-journal databases were searched, and 71 articles were selected for review according to the inclusion criteria. RESULTS AND CONCLUSION: (1) Mesenchymal stem cells, liver stem cells, embryonic stem cells, induced pluripotent stem cells and hematopoietic stem cells are the main stem cells for the treatment of liver diseases. The characteristics, expression period and application of these stem cells in differentiation after liver injury are summarized in this paper. (2) In general, mesenchymal stem cells can repair liver through exosomes, matrix metalloproteinase and anti-inflammatory response. Liver stem cells can differentiate liver oval cells and small hepatocellular progenitor cells to form mature liver cells and promote liver injury repair. Induced pluripotent stem cells, embryonic stem cells and hematopoietic stem cells can transform into mature hepatocytes through rapid proliferation and differentiation. (3) The treatment of various liver diseases by stem cells is mainly achieved by inducing endogenous cell proliferation, immune regulation, inhibiting inflammation, promoting liver cell regeneration and repairing damaged tissues. (4) On the whole, at present, mesenchymal stem cells are relatively easy to obtain, have the broadest application prospects, and do not involve ethical issues. They are transforming from preclinical research to clinical research. Because mesenchymal stem cells can differentiate into various adult stem cells, they have the advantages of low immunogenicity, strong self-proliferation and differentiation ability. Especially for the treatment of immune hepatitis, liver cirrhosis and hepatocellular carcinoma and other liver diseases, mesenchymal stem cells have the most ideal effect. (5) Induced pluripotent stem cells are more effective in the treatment of end-stage liver disease because they can differentiate into specific somatic cells and hepatocytes without immune rejection. (6) However, stem cell transplantation can induce acute rejection, such as diarrhea, jaundice, and skin rashes. Poor histocompatibility between donors and recipients can easily lead to adverse reactions such as graft-versus-host reactions. After stem cell transplantation, the use of stem cells and its clinical safety assessment still need the support of more clinical trials and evidence-based medicine data. [ABSTRACT FROM AUTHOR]

Bryophytes are important indicators of environmental pollution. At present, bryophytes are mainly used to monitor atmospheric pollution in China. In fact, bryophytes have been widely applied to monitor water quality abroad. In the present paper, we firstly analyzed the literature of applying bryophytes to monitor water environmental quality abroad, then summarized relevant contents of bryomonitoring, which included the types of water pollutants monitored by bryophytes, the species of bryophytes used for bryomonitoring, the influences of the survival status of bryophytes on monitoring effect, the environmental factors affecting the enrichment of heavy metal elements in water by bryophytes, the active and passive methods of applying bryophytes to monitor water pollutions, and the sample preparation, as well as some European cases of bryomonitoring for water environmental quality. Finally we also proposed some suggestions about bryomonitoring researches and application in the future. [ABSTRACT FROM AUTHOR]

Alzheimers disease (AD) is a disease with complex pathogenesis. With the increase of the aging population in China, the incidence of AD is gradually increasing, which has seriously affected the life quality and health of the elderly. The establishment of a suitable pathological model is of great significance for the study of the pathogenesis of AD and the mechanism involved in drug prevention and treatment. In recent years, the application of metabolomics in the investigation of pathogenesis of AD has been deepened. This paper makes a comprehensive review of the existing pathological models of AD including animal models and cell models, as well as the application of metabolomics in AD research in recent years, so as to provide reference for future research. [ABSTRACT FROM AUTHOR]