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Objective To explore the surface landscape characteristics, evolution patterns and epidemiological significance of the Himalayan dry otter plague natural epidemic sites under the dual influence of natural environment and major projects such as the Sichuan-Tibet Railway. Methods Based on the continuous land use, NDVI and other geographic environmental data of the natural source of Himalayan dry otter plague along the Sichuan-Tibet Railway in Sichuan from 2010 to 2021, paper quantitatively analyzed the changes in the geographic pattern of the natural source of plague and its association with the Himalayan dry otter population with the help of land use shift matrix, NDVI trend analysis and landscape index. Results During 2010-2020, the dominant vegetation type is grassland, the transfer between various types of land is gentle, the overall vegetation cover rises gently by 24.78%, the area of vegetation improvement zone accounts for 75.84%, the aggregation of various types of grassland strengthens, the differentiation of land classes intensifies, and the density of Himalayan dry otter decreases. After 2020, the transfer of land is relatively intensified, mainly the conversion between forest land and grassland, the scattered expansion of various types of grassland, and the density of Himalayan dry otter decreases. The boundary between classes gradually weakens, and the density of Himalayan dry otter increases. Conclusion Dual natural and anthropogenic disturbances will intensify changes in the landscape pattern of the natural source of Himalayan dry otter plague along the Sichuan-Tibet Railway, destroy the overall stability of the ecosystem of the natural source of plague. [ABSTRACT FROM AUTHOR]