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Background Traditional Chinese medicine (TCM) is effective in the treatment of sepsis-induced acute lung injury (ALI) /acute respiratory distress syndrome (ARDS). However, the lack of standard and uniform outcome indicators in the design of most published clinical trials makes it difficult to combine and compare the results of similar studies, causing the impossibility of generating high-quality evidence to guide clinical decision-making. Objective To analyze the use of outcome indicators in randomized controlled trials on TCM for sepsis-induced ALI/ARDS from 2017 to 2022. Methods CNKI, Wanfang Data, SinoMed, PubMed, Embase, Web of Science and Cochrane Library were searched for RCTs on sepsis-induced ALI/ARDS, with the intervention group treated with TCM combined with other drugs or conventional treatment, and no restrictions in the control group. The ROB.2 tool of the Cochrane Review Group was used to assess the quality of literature and the classification of outcome indicators was summarized. Results 39 papers were included after screening, including 1 (2.56%) with low risk of bias, 3 (7.69%) with high risk of bias and 35 (89.74%) with concerns of bias. The outcome indicators in 5 RCTs were reported with a quality score of ≥5, indicating the complete reporting of outcome indicators. A total of 106 outcome indicators were reported and used for 443 times, including physicochemical testing outcome (55 types, 51.89%), TCM syndrome/sign outcome (2 types, 1.89%), symptom and syndrome outcome (13 types, 12.26%), long-term prognosis outcome (19 types, 17.92%), quality of life outcome (7 types, 6.60%), safety event outcome (8 types, 7.55%) and economic evaluation outcome (2 types, 1.89%). There were four outcome indicators with utilization rate>50%, including oxygenation index (82.05%), Acute Physiology and Chronic Health Evaluation Scoring System (58.97%), mechanical ventilation duration (51.28%) and interleukin-6 (51.28%). The composite outcome indicators of effective rate or TCM syndrome efficacy were used in 21 papers, involving a total of 12 times of effective rate, TCM syndrome score of 8 times and TCM symptom efficacy of 6 times. The total 106 outcome indicators were summarized and categorized sequentially including overall evaluation indicators (36 types, 33.96%), common indicators (36 types, 33.96%) and different focus indicators (34 types, 32.08%) . Conclusion At present, the selection of outcome indicators in RCTs on TCM for sepsis-induced ALI/ARDS is still nonstandard, mainly manifests in unclear differentiation between primary and secondary outcome indicators, trivialization of endpoint indicators, lack of follow-up data and characteristic indicators of TCM. A core outcome set of TCM outcome indicators for septic induced ALI/ARDS should be constructed to promote the high-quality development of TCM in the future.

Under atmospheric pressure, KOH or HCl solution was added to the butylated hydroxytoluene (BHT) oxidation system to adjust the pH, and the oxidative consumption of BHT was monitored by high performance liquid chromatography (HPLC). To investigate the oxidation properties of BHT under pressurized conditions, the temperature and pressure changes during the oxidation process were monitored using the mini-closed pressure vessel test (MCPVT). The oxidation products of BHT were characterized after crystallization and purification and the possible oxidation pathway was speculated. The results showed that under atmospheric pressure, the degradation rate of BHT was less than 5% when the pH was 4 or 6, indicating good stability. At pH 10, the degradation rate of BHT was up to 59% and the oxidation reaction was rapid. KOH and HCl decreased the initial oxidation temperature of BHT from 133.1 ℃ to 53.0 ℃ and 49.1 ℃ in a pressurized closed container, respectively. 2, 6-Di-tert-butyl-4-hydroperoxy-4-methyl-2, 5-cyclohexanedione and 2, 6-di-tert-butyl-4-methyl-4-(3, 5-di-tert-butyl-4-hydroxyphenyl)-2, 5-cyclohexadien-1-one were produced during the oxidation of BHT in both atmospheric alkaline and pressurized environments. The experimental results suggested that BHT was susceptible to oxidative degradation in alkaline and pressurized environments, producing toxic products, posing safety risks.

The change of illumination have a serious effect on pedestrian detection algorithms based on image difference, general adaptive background updating methods is difficult to eliminate the effects of illumination change. This paper proposed a new method of illumination changes elimination based on similarity of histogram distribution to solve this problem. It analyzed similarity of histogram distribution on local area between illumination changed image and normal image. It presented standard deviation of image which could use to measure similarity of histogram distribution. Through image difference experiment between two adjacent frames acquired under light frequency change environment, it shows that the method has a outstanding performance in robustness which can segment the moving targets accurately and restrain the changes of illumination significantly. [ABSTRACT FROM AUTHOR]

Orthotopic xenograft model of human brain cancer cells is a good preclinical model for evaluation of antitumor compounds. In the present study, an orthotopic xenograft model of U87MG-m Cherry-luc was established in Balb/c nude mice and the tumor growth was monitored via imaging technology including magnetic resonance imaging (MRI), in vivo imaging (IVI) and micro-CT. Furthermore, the model was evaluated with a positive drug temozolomide. Our data suggest that integrated imaging technology including MRI, IVI and micro-CT in orthotopic xenograft model can be used to facilitate monitoring of cancer progression and evaluate antitumor activity of drugs against glioma.

Poly (ADP-ribose) polymerase 1/2 (PARP1/2) can catalyze the poly (ADP ribose) (PAR) substrate protein modification and play an important role in the regulation of DNA damage repair, cell death and transcriptional activity. The PARP inhibitor olaparib (AZD2281) can be used as a sensitizer of radiotherapy and chemotherapy in the cancer treatment. Through establishment of biological fluorescent labeled 4T1 ectopic breast tumor model, we found that olaparib exhibited a poor effect on 4T1 breast cancer alone. However, in the combination with Taxol, olaparib significantly increased the anti-tumor effect of Taxol, and reduced the PAR levels of the tumor tissues. Importantly, olaparib did not amplify the toxicity of chemotherapy drugs. This study suggests that olaparib is a representative of the PARP inhibitor that can enhance Taxol’s antitumor effect in the 4T1 ectopic breast tumor model, which sets the foundation for future study of the mechanism of olaparib action.