1. 绿原酸通过 Bax / Bcl-2/ Caspase-3 信号通路对喉癌 Hep-2 细胞增殖、凋亡的影响.
- Author
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张立坤, 邵东风, 王东海, and 李 扬
- Subjects
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BAX protein , *BCL-2 proteins , *LARYNGEAL cancer , *CANCER cells , *CHLOROGENIC acid , *RATES , *CURCUMIN - Abstract
OBJECTIVE: To probe into the effects of chlorogenic acid (CGA) on proliferation and apoptosis of laryngeal cancer Hep-2 cells through Bcl-2-associated X protein ( Bax ) / B cell lymphoma-2 ( Bcl-2) / cysteinyl aspartate specific proteinase-3 (Caspase-3). METHODS: Laryngeal cancer Hep-2 cells were treated with CGA culture medium of 0, 250, 500, 750 and 1 000 μmol / L for 24 and 48 h respectively to find out the most suitable concentration and treatment time of CGA. Laryngeal cancer Hep-2 cells were divided into the control group (normally cultured laryngeal cancer Hep-2 cells), CGA low concentration group ( 750 μmol / L CGA medium), CGA high concentration group ( 1 000 μmol / L CGA medium ), and CGA high concentration + pathway inhibitor group (1 000 μmol / L CGA medium+50 μmol / L Bax / Bcl-2/ Caspase-3 pathway inhibitor Bax inhibitor peptide V5). Cell counting kit-8 (CCK-8) method was used to determine the proliferation inhibition rate of laryngeal cancer Hep-2 cells in each group, flow cytometry was used to detect the apoptosis rate of laryngeal cancer Hep-2 cells in each group, Western blotting was used to determine the expression of proliferation related protein (ki-67), apoptosis related protein (p53) and Bax / Bcl-2/ Caspase-3 signaling pathway related proteins in laryngeal cancer Hep-2 cells. RESULTS: Compared with the control group, the proliferation inhibition rate, apoptosis rate, p53, Bax and Caspase-3 protein expression levels of laryngeal cancer Hep-2 cells in CGA low and high concentration group were significantly higher, and the ki-67 and Bcl-2 protein expression levels were significantly lower, with statistically significant differences (P< 0. 05); and the proliferation inhibition rate, apoptosis rate, p53, Bax and Caspase-3 protein expression levels of laryngeal cancer Hep-2 cells in CGA high concentration group were higher than those in CGA low concentration group, the of ki-67 and Bcl-2 protein expression levels were lower than those in CGA low concentration group, with statistically significant differences (P < 0. 05). Compared with the CGA high concentration group, the proliferation inhibition rate, apoptosis rate, p53, Bax and Caspase-3 protein expression levels of laryngeal cancer Hep-2 cells in the CGA high concentration+pathway inhibitor group were significantly lower, and the ki-67 and Bcl-2 protein expression levels were significantly higher, with statistically significant differences ( P < 0. 05). CONCLUSIONS: CGA can significantly inhibit the proliferation of laryngeal cancer Hep-2 cells and promote the apoptosis, which may be related to the activation of Bax / Bcl-2/ Caspase-3 signaling pathway. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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