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A virtual simulation software, which is also called "electronic book" for experimental teaching of capillary electrophoresis has been independently developed. It was programmed in Web development language. Other auxiliary tools such as Photoshop, Flash and 3D Max software were used to make a realistic interactive three-dimensional model of virtual instruments. The mentality of designing, function module, applied method and teaching efficiency of this virtual simulation experimental software were also introduced and discussed. This "electronic book" contained the detailed steps of the experimental process, i.e., the experimental principle, introduction to instruments, sample preparation, parameter setting, sample testing, data analysis and experimental report writing. Because it completely matches the current real experiment and textbook in paper form, the online-merge-offline hybrid experimental efficiency and the combination of "real experiment" with "virtual experiment" can be achieved. Through rich and colorful pictures, animation, 3D drawing in electronic book, it greatly helps students master the relevant knowledge of the instrument analytical testing items in an all-round way, effectively solving the current problems of traditional experimental teaching. Moreover, it motivates the students' enthusiasm and consciousness, and enhances the interest and efficiency of experimental teaching. [ABSTRACT FROM AUTHOR]