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Objective The study was aimed to observe the success rate of streptozotocin (STZ) induced diabetic rats by low dose repeated and large dose with a single intraperitoneal injection, and explore the ideal scheme of STZ induced diabetic rats. Methods 48 healthy male SD rats were randomly divided into 6 groups: group 1 (20 mg/kg), group 2 (30 mg/kg), group 3 (40 mg/kg), group 4 (50 mg/kg), group 5 (60 mg/kg) and group 6 (the control group) . The groups 1-3 were treated with repeated intraperitoneal injection scheme, and each injection interval time was 5 days. The groups 4 and 5 were conducted according to the single intraperitoneal injection scheme. Blood glucose was measured at the fifth day after each injection. Results The rats model of group 2, group 3, and groups 4 and 5 was successfully established after four times, two times and one time of injection, respectively. At the end of the experiment, the success rate of groups 2 to 5 was 75%, 100%, 75% and 75%, respectively. In group 1, the model has not yet succeeded after five times injection. In group 5, 2 rats died. Conclusion The single intraperitoneal injection of STZ 50 mg/kg was an economical approach to induce diabetic SD rats, which has high success rate and survival rate. [ABSTRACT FROM AUTHOR]