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In this paper, a random copolymer of N, N-dimethylacrylamide and N-isopropylacrylamide (P (DMA-coNIPAM) ) as well as poly-N, N-dimethylacrylamide (PDMA) are synthesized and blended to prepare a composite sieving medium for non-gel capillary electrophoresis, aiming to reduce the viscosity of the sieving medium while enhance its DNA separation performance. Analyses by NMR spectroscopy and Fourier transform infrared FT-IR) spectroscopy confirm that both polymers are successfully synthesized. The hydrodynamic diameter, low shear viscosity and UV-Vis absorbance results show that P (DMA-co-NIPAM) is thermoresponsive with a low critical solution temperature (LCST) of 60~80 ℃ . DNA sieving results indicate that the composite medium containing 10% P (DMA-co-NIPAM) exhibits best properties, a viscosity 19% lower than PDMA and a higher resolution and theoretical plate number in separating large DNA fragments, and separation time is reduced by more than 4%, showing good separation performance. [ABSTRACT FROM AUTHOR]

A virtual simulation software, which is also called "electronic book" for experimental teaching of capillary electrophoresis has been independently developed. It was programmed in Web development language. Other auxiliary tools such as Photoshop, Flash and 3D Max software were used to make a realistic interactive three-dimensional model of virtual instruments. The mentality of designing, function module, applied method and teaching efficiency of this virtual simulation experimental software were also introduced and discussed. This "electronic book" contained the detailed steps of the experimental process, i.e., the experimental principle, introduction to instruments, sample preparation, parameter setting, sample testing, data analysis and experimental report writing. Because it completely matches the current real experiment and textbook in paper form, the online-merge-offline hybrid experimental efficiency and the combination of "real experiment" with "virtual experiment" can be achieved. Through rich and colorful pictures, animation, 3D drawing in electronic book, it greatly helps students master the relevant knowledge of the instrument analytical testing items in an all-round way, effectively solving the current problems of traditional experimental teaching. Moreover, it motivates the students' enthusiasm and consciousness, and enhances the interest and efficiency of experimental teaching. [ABSTRACT FROM AUTHOR]

[Objective] The present paper aimed to address the lack of SSR molecular markers in Illicium verum, based on I. verum transcriptome data, EST-SSR molecular markers were developed and the distribution types and characteristics in transcriptome sequences were revealed. The purpose of this study was to lay a foundation for the study of I. verum at molecular level. [Method] SSR loci were screened from I. verum transcriptome data by SSR search program, and the types, quantity and distribution characteristics of SSR repeat motifs were subjected to statistical analyses. An online design software was used to design SSR primers, and the polymorphism result from primers was evaluated by capillary electrophoresis. The genetic relationship between different I. verum germplasm was analyzed by using selected polymorphic primers. [Result] 8210 SSR loci were obtained from 44 267 Unigenes, The proportion of one EST-SSR loci was 11.11%, the proportion of two or more EST-SSR loci was 3.13%, and the proportion of composite SSR loci was 2.09%. The frequency of SSR was 14.24%, with an average of one SSR every 5.37 kb. Dinucleotides and trinucleotides were the main repeat types among the SSR loci, the highest proportion of dinucleotide repeats was AG/CT, which accounted for 46.03%, the highest proportion of trinucleotide repeats was GAA/CTT, which accounted for 16.58%, and the repetition types and proportion of four, five and six nucleotides were relatively low. The number of replicates of the SSR repeat units ranged from 4 to 40 times, and 5 repetitions were the most, accounting for 22.58%, Then there were 6 repetitions, accounting for 19.83%. The length distribution of microsatellites rangeed from 12 to 80 bp, and 12-15 bp was the most, accounting for 37.39%. Sixteen pairs of primers with high polymorphism were screened by amplification of 100 pairs of primers randomly selected. Na was 3 to 8, Ho was 0.13 to 0.88, He was 0.54 to 0.88, Ne was 2.03 to 5.82, I was 0.83 to 1.84. The primer polymorphism information index was 0.43 to 0.81, with an average of 0.63, indicating that the developed EST-SSR loci had higher polymorphism. The genetic relationship indicated that there were different genetic relationships among different I. verum germplasm. [Conclusion] It is feasible to develop EST-SSR markers based on I.verum transcriptome sequences, and its primers have the advantages of high specificity and high polymorphism, the developed SSR markers can provide basis for genetic diversity analysis, functional gene mining, genetic linkage map construction. [ABSTRACT FROM AUTHOR]