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Tested were a number of procedures to employ the immunofluorescence method for diagnosing transmissive gastroenteritis in pigs--impression preparations, frozen cross-sectioned material, and infected cell cultures of the established cell line SPEV. It was found that immunofluorescence microscopy was a dependable method for the express diagnosis of transmissive gastroenteritis. It could be employed in its three variants. The use of impression preparations, however, did not prove as dependable as the remaining ways of applying the method, and this drawback had to be compensated for with the study of a greater number of impression preparations taken from more pigs that had contracted the disease. It was also established that most promising and effective was to apply the method with the use of cell cultures infected with suspensions of organs. Cell cultures of the established SPEV cell line infected with material that contained the virus could produce dependable positive results in immunofluorescence investigations at the 24th hour following inoculation. This method could be employed for the express diagnosis of transmissive gastroenteritis.

Fluorescence microscopy was employed to study Dictyocaulus infection in 15 experimentally infected lambs each given 2000 D. filaria larvae. Studied were also the sera of 10 control lambs and of 20 sheep and goats infected with other helminths. Antibodies were established after the 14th day in 53 per cent of the lambs, the 20th day in 86.6 per cent, and the 27th day in all animals infected with the parasite. Antigens were found mainly in the wall of the digestive and reproductive organs, and in the cuticle of the larvae. As a method of early diagnosis fluorescence microscopy is simple, precise, and readily applicable in establishing Dictyocaulus infections.

Bisbenzimid-marked continuous cell lines were investigated via fluorescent microscopy. The presence of Mycoplasma DNA was demonstrated in the cytoplasm with some of them. The method employed for the demonstration of Mycoplasma contamination is readily applicable, and could be used for control on continuous cell lines.