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In the present study, two methods were used to extract oil from seeds of Sardasht black grapewith normal-hexane and cold press.The average efficiency rate of oil extraction by the formermethod was obtained at 12.6% and that of the latter was 17.2%. Meanwhile, in order to examine the quality of the oilsextracted by these two methods, peroxide value, acid value, sapponification number, total phenolic contents, antioxidant power and iodine valuewere evaluated. The compositions of fatty acids of oil samples extracted by both methods were identified using gas chromatography technique. Similarly, the triglyceride composition, phospholipids composition and tocopherol values of the oil samples extracted were studied. The results showed that the dominant fatty acids available in cold-pressand solvent extraction methods were linoleic acid at 63.5 and 66.7% and oleic acid at 19.7 and 17.7%, respectively. Moreover, in both extracted oil samples, trilinoleinwith values at 28.9 and 28.5%, oleoyldilinolein with values at 19.5 and 20.5%and palmitoyldilinolein with values at 18.5 and 17.1% were the dominant triglyceride compounds, respectively. The value of phospholipids composition in the oil extracted by cold-presswas 61 mg/kg and that of solventextractionwas 45mg/kg. The dominant values of tocotrienol compositions in oil samples extracted by coldpresswere gamma tocotrienol and alpha tocotrienol at 66.0 and 23.1%, respectively and the corresponding values in oil samples extracted by solvent method were at 33.3 and 53.0%, respectively. [ABSTRACT FROM AUTHOR]