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Background and Objectives One of the applications of Epstein-Barr virus in research is the immortalization of B lymphocytes and the creation of the lymphoblastoid cell line. People with thalassemia usually need blood transfusion, the main complication of which is alloimmunization. Antibody against RhE antigen causes hemolytic reactions. The aim of this study is to create a lymphoblastoid cell line that produces anti-E using peripheral blood mononuclear cells of alloimmune thalassemia patients against E antigen when exposed to Epstein Bar Virus (EBV). Materials and Methods In an experimental study, the mononuclear cells of 3 patients were isolated by Ficol and mixed with EBV. The transformed cells were cultured in RPMI medium containing cyclosporine. After 2-3 weeks, the formation of clones in the wells was checked and the hemagglutination test was performed for the wells containing the growing cells; the total amount of antibody in positive wells was measured by ELISA method. Results Exposure of mononuclear cells of patients with EBV led to cell transformation and creation of clones in some wells. The results of hemagglutination and ELISA methods showed that the clones were able to produce antibodies against the RhE antigen. Conclusions Anti-E antibody-producing lymphoblastoid cell line can be created by using sensitized cells of thalassemia patients against RhE antigen and its immortalization by EBV transformation method. [ABSTRACT FROM AUTHOR]

Introduction: lncRNAs play their roles in cell growth, transcription, translation, and apoptosis. UCA1is a type of lncRNA has a direct relationship with ovarian cancer. More than half of the women with ovarian cancer die within less than five years from the disease diagnosis. This study aims to knockout of UCA1gene using the CRISPR/Cas9 technique in SK-OV-3 cell and studying its effect on cell factors involved in cancer progress and apoptosis. Materials and Methods: Two types of sg RNAs for UCA1gene were designed. sgRNAs were cloned after synthesis in two vectors of PX459. The SK-OV-3 cell was transfected with two vectors carrying sgRNAs. The expression of FAS, BAK, BAX, and P53pro-apoptosis genes and BCL2 and SURVIVIN anti-apoptosis genes were evaluated using the q-PCR. Besides, the cell proliferation rate and apoptosis induction were studied using MTT and flowcytometry. Results: The result showed the successful knockout of the UCA1gene in the SK-OV-3 cell line. The expression of FAS, BAK, BAX, and P53 genes showed a meaningful increase related to the expression in manipulated cells compared to control cells (p˂0.05). Also, the expression decrease of BCL2and SURVIVIN genes was seen (p˂0.05). MTT and flow cytometry results confirmed the cell proliferation decrease and the apoptosis increase.Conclusion:UCA1gene Knockout in the SK-OV-3 showed some positive effects in decreasing cancer cell proliferation and increasing the apoptosis rate can be said that inhibiting overexpression of the UCA1gene will play a positive role in controlling the growth of ovarian cancer cells [ABSTRACT FROM AUTHOR]

Despite advances in diagnosis and therapy of cancer, it is still the main cause of death worldwide. Cyclophosphamide is one of the major drugs for lymphoma treatment. However, the anti-lymphoma effects of cyclophosphamide on micro-RNA expression are still unclear, especially in B cell lymphomas. The aim of the present study was to evaluate the effect of cyclophosphamide on miRNA15a genes expression in Epstein‐Barr‐Virus (EBV)-infected transformed B cell. Material and Methods: In this study, the effects of cyclophosphamide on CO 88BV59-1 LCL – an EBV infected transformed B cell was evaluated. The cells were treated with cyclophosphamide (0.05-50 µM), for 3 days. The cell viability and cytotoxicity were evaluated using MTT, and flow cytometry techniques, respectively. Also, miRNA-15a genes expression were determined by real-time PCR. Results: Cyclophosphamide significantly inhibited proliferation and induced cell death in the cell-line in a dose and time-dependent manner (P<0.05). There were no significant changes in the expression of miRNA15a3p and miRNA-15a5p in treated cells compared to untreated cells (P>0.05). Conclusions: The results showed that cytotoxic effects of cyclophosphamide on CO 88BV59-1 LCL is not related to miRNA-15a3p and miRNA-15a5p expressions. [ABSTRACT FROM AUTHOR]

The phytochemical studies on Zingiber officinale rhizomes revealed that it contains traces of flavonoid, carbohydrates, tannins, steroles or terpenoids and it is free from alkaloids.Assay of essential oil of Zingiber officinale rhizome was also carried out. The alcoholic extract of the plant rhizome, was examined against five tumor cell lines, BHK-2, HEPG-2, MCF-7, HCT-2 and HEP-2, using SRB. assay. All cell lines were growth inhibited in a dose dependent manner after exposure to the plant extract. The antitumor activity of the plant extract using E.A.C. method, showed a high activity against mice tumor.

Background and Objective: Chrysin is a natural and active biological component which is extracted from plants, honey and propolis. Chrysin has anti-inflammatory, anticancer and antioxidant propertis. This study was done to evaluate the effect of chrysin on AGS human gastric cancer cell line. Methods: In this descriptive - analytic study, chrysin was dissolved in dimethyl sulfoxide (DMSO) and the cytotoxic effects of concentrations of 10, 15, 20, 30, 40, 50, 60, 70, 80, and 100M/ml of chrysin on AGS cells was evaluated. Viability of the cells was determined with MTT assay after 24, 48 and 72 hours and compared to controls. Results: Chrysin inhibited the growth and proliferation of human gastric cancer AGS cell line. The antiproliferative effect of chrysin was dose and time dependent. The IC50 values were determined for 60, 30 and 20M, in incubation time of 24, 48 and 72 hour, respectively (P<0.05). Conclusion: Chrysin proved to have antiproliferative activity on human gastric cancer cells in culture medium. [ABSTRACT FROM AUTHOR]

Background: The aim of this study was to evaluate the effect of extracted cell walls from Lactobacillus casei and Lactobacillus paracasei as probiotic bacteria (isolated from common carp intestine) on K562 and the role of cell concentration on the results of MTT [3-(4,5-Dimethylthiazol-2-yl)2,5- Diphenyl tetrazolium Bromide] test. Methods: For this purpose, bacteria were cultured in specific medium (MRS broth) at anaerobic condition for 24-48 hour. After incubation period culture medium was centrifuged, then the cells were washed twice with PBS buffer to remove additional medium. Finally, collected bacterial cell disrupted by Sonication and cell walls were separated from other components by centrifugation. After that, different concentrations of cell walls (500, 1000, 2000 and 4000 μg/ml) were prepared in RPMI medium for each bacteria, separately. Then anticancer properties of the cell walls were determined in vitro at 12, 24, 48 and 72 h, also the effect of K562 concentration was assayed with MTT technique. Results: The results showed extracted cell wall from both probiotic statistically (P=0.098) have anti turmeric properties in K562 and their properties will arise in relation with concentration. As well as, we found that the number of cell had not any affect on the result of MTT assay. Conclusion: We conclude that the cytotoxicity property of extracted cell wall is related in the type of bacteria, but this anticancer property would warrant further study on the clinical application of extracted cell wall. [ABSTRACT FROM AUTHOR]

Background: Correct use of cuminum cyminum and finding its different applications in medicine and industry necessitates a more clear understanding of this medicinal plant. This study deals with identifying the effects of the essential oil of this herbal plant on macrophages and tumor cell lines. Materials and Methods:Cuminum cyminum essential oil was extracted from its fruit, and its effects on peritoneal macrophages and LPS stimulated macrophages were examined. MTT assay was done for evaluation of macrophages viability and the amount of nitric oxide (NO) in culture supernant was measured by Griess Reagent. WEHI-164 mice fibrosarcoma cell line was cultured with different concentrations of cuminum cyminum and cytotoxicity level was evaluated by MTT assay. Results: The viability of macrophages and also, the amount of NO production in 50 and 500 μg/ml cuminum cyminum essential oil was lower than that of the control group (p<0.001). MTT assay showed that cuminum cyminum essential oil in 50 and 500 μg/ml concentrations significantly inhibits tumor cells growth (p<0.001). Conclusion: Cuminum cyminum essential oil by having immune-modulatory properties can be used in treatment of many inflammatory and immunologic disorders. Also, it can be used as a therapeutic or complementary agent in tumor therapy. [ABSTRACT FROM AUTHOR]

Background: Vascular endothelial growth factor (VEGF) has mitogenic effect for endothelial cells and is an important mediator of tumor expansion, metastasis and angiogenesis in vivo. Isosorbide dinitrate, as a nitric oxide donor, has been widely used in treatment of many cardiovascular diseases such as congestive heart failure and acute coronary syndromes. Furthermore this drug was found to have inhibitory effect on angiogenesis, tumor growth and metastasis in vivo. In the present study we evaluated the isosorbide effect on the VEGF production using some human leukemic cell lines. Methods: Human leukemic MOLT-4, JURKAT and U937 cells were cultured in complete RPMI medium. The cells at the exponential growth phase were then incubated with different concentrations of Isosorbide (4×10-7 -4×10-4 M) in the presence or absence of PMA (25ng/ml) for 24 hours. The VEGF concentrations in the culture supernatants were measured by enzyme immunoassay kits (R&D systems) according to the manufacturer's instructions. Results: The level of VEGF produced by the human leukemic cell lines which was treated with different concentrations of isosorbide, did not show any significant difference with untreated control cells. Conclusions: The results of this study showed that isosorbide had no significant effect on VEGF production. Our findings suggest that anti-angiogenesis effect of isosorbide could be mediated through VEGF-independent mechanism(s). Further studies are warranted to determine definite isosorbide effect on VEGF and other angiogenic factors production in patients as well as animal models. [ABSTRACT FROM AUTHOR]