1. [Role of c-Jun NH (2)-terminal kinase in insulin resistance after burn]
- Author
-
Xin-long, Chen, Zhao-fan, Xia, Duo, Wei, Dao-feng, Ben, Hong-tai, Tang, and Sheng-de, Ge
- Subjects
Male ,Muscles ,Blotting, Western ,JNK Mitogen-Activated Protein Kinases ,Immunohistochemistry ,Anti-Bacterial Agents ,Rats ,Rats, Sprague-Dawley ,Disease Models, Animal ,Random Allocation ,Injections, Intravenous ,Glucose Clamp Technique ,Insulin Receptor Substrate Proteins ,Serine ,Animals ,Insulin ,Tyrosine ,Dimethyl Sulfoxide ,Female ,Insulin Resistance ,Phosphorylation ,Burns ,Anisomycin ,Adaptor Proteins, Signal Transducing - Abstract
To investigate the role of c-Jun NH (2)-terminal kinase (JNk) in insulin resistance after burn and its mechanism.Twenty-four Sprague-Dawley rats were randomized to control, burn and burn + anisomycin groups. The rats in control group received sham burn trauma, and burn and burn + anisomycin groups received 30% total body surface area (TBSA) full thickness burn injury. Anisomycin (5 mg/kg) together with 250 microl dimethyl sulfoxide (DMSO) was injected to the rats in anisomycin group intravenously, and only 250 microl DMSO in the other two groups. Euglycemic-hyperinsulinemic glucose clamps was performed 2 hours after the injection. The changes of phospho-serine 307, phospho-tyrosine of insulin receptor substrate (IRS)-1 and phospho-JNK in muscle tissues were determined and compared using immunoprecipitation and Western blot analysis or immunohistochemistry in the three groups.The infusing rates of total 10% glucose (mg x kg(-1) x min(-1)) in control, burn and burn + anisomycin group were 12.3 +/- 0.4, 6.6 +/- 0.3, 6.5 +/- 0.4, respectively. The level of IRS-1 Serine 307 phosphorylation and phospho-JNK in muscle increased significantly, while insulin-induced tyrosine phosphorylation of IRS-1 decreased markedly after burn.The activation of JNK elevates the level of IRS-1 phospho-serine 307 and might play a role in insulin resistance after burn in rats.
- Published
- 2007