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Background: Family practice is an important setting for health promotion and prevention in Germany. However, there is a research gap regarding the involvement of nonmedical practice staff in health promotion. Therefore, our aim is to gain an indepth understanding of the role health care assistants (HCAs) can play regarding health promotion in family practice. Methods: We conducted 21 qualitative semi-structured interviews via telephone with 14 physicians and 7 HCAs, inquiring both professional groups about the role of HCAs concerning health promotion in their practice. The interviews were evaluated separately for both professions using thematic analysis. Results: In addition to organizational tasks such as making an appointment and communicating with physicians, HCAsmainly take on a key function as the first contact person in all practices: They recognize preventive needs, select eligible patients early at the reception and actively address them about preventive services. In some practices, HCAs carry out health-promoting measures such as training courses. However, with regard to further delegation of tasks, physicians in particular expressed concerns as they did not want to overburden HCAs. Conclusions: HCAs often take on organizational activities with regard to health promotion in family practice, but due to their function in the practice structure, they can also shape the practice as a health-promoting setting for patients. In addition, HCAs navigate patients with regard to preventive and health-promoting services. In accordance with their key role as initial contact, it should be worked out together with HCAs how health promotion can be addressed in a structured way at first contact. [ABSTRACT FROM AUTHOR]