1. The ORF YNL274c (GOR1) codes for glyoxylate reductase in Saccharomyces cerevisiae
- Author
-
Juha-Pekka Pitkänen, Maija-Leena Vehkomäki, Laura Ruohonen, Eija Rintala, and Merja Penttilä
- Subjects
Saccharomyces cerevisiae ,Glyoxylate cycle ,Bioengineering ,Dehydrogenase ,Biology ,Models, Biological ,Polymerase Chain Reaction ,Applied Microbiology and Biotechnology ,Biochemistry ,Open Reading Frames ,Genetics ,DNA, Fungal ,Gene ,Glyoxylate reductase ,chemistry.chemical_classification ,Glyoxylates ,Hydroxypyruvate ,Hydroxyisocaproate ,biology.organism_classification ,Alcohol Oxidoreductases ,Mutagenesis, Insertional ,Enzyme ,chemistry ,Biotechnology - Abstract
The enzyme glyoxylate reductase reversibly reduces glyoxylate to glycolate, or alternatively hydroxypyruvate to D-glycerate, using either NADPH or NADH as a co-factor. The enzyme has multiple metabolic roles in different organisms. In this paper we show that GOR1 (ORF YNL274c) encodes a glyoxylate reductase and not a hydroxyisocaproate dehydrogenase in Saccharomyces cerevisiae, even though it also has minor activity on α-ketoisocaproate. In addition, we show that deletion of the glyoxylate reductase-encoding gene leads to higher biomass concentration after diauxic shift. Copyright © 2006 John Wiley & Sons, Ltd.
- Published
- 2007