1. Genotoxic activity of l-asparaginase produced by Streptomyces ansochromogenes UFPEDA 3420
- Author
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Mônica Lúcia Adam, Isllan D Eric Gonçalves da Silva, Luiz Eduardo Felix de Albuquerque, Maria Eliane Bezerra de Melo, Jeanne Cristina Lapenda Lins Cantalice, Gláucia Manoella de Souza Lima, Glêzia Renata da Silva Lacerda, and Silene Carneiro do Nascimento
- Subjects
0106 biological sciences ,Physiology ,Antineoplastic Agents ,medicine.disease_cause ,01 natural sciences ,Applied Microbiology and Biotechnology ,Peripheral blood mononuclear cell ,Inhibitory Concentration 50 ,03 medical and health sciences ,Cell Line, Tumor ,010608 biotechnology ,Aspartic acid ,medicine ,Asparaginase ,Humans ,Asparagine ,Enzyme Assays ,chemistry.chemical_classification ,Aspartic Acid ,0303 health sciences ,Micronucleus Tests ,030306 microbiology ,General Medicine ,Molecular biology ,Streptomyces ,Enzyme ,chemistry ,Mechanism of action ,Leukocytes, Mononuclear ,Neoplastic cell ,medicine.symptom ,Micronucleus ,Genotoxicity ,DNA Damage ,Biotechnology - Abstract
L-asparaginase is an enzyme capable of hydrolyzing the substrate asparagine in aspartic acid and ammonia. Due to this mechanism of action observed, L-asparaginase is widely used in the treatment of Acute Lymphoblastic Leukemia, since these cells use asparagine for their survival. Because it is frequently used as an antineoplastic, it is necessary to evaluate its genotoxic effects. The aim of the present study was to evaluate cellular DNA damage after exposure to L-asparaginase produced by Streptomyces ansochromogenes UFPEDA 3420. NCIH-292, MCF-7 and MOLT-4 neoplastic cell lines and normal PBMC cells were used. L-Asparaginase used in this study was produced by actinobacteria S. ansochromogenes UFPEDA 3420, isolated and purified by chromatographic methods. L-Asparaginase induced micronucleus formation in PBMC cells and tumor lines when compared to the negative control. These data suggest that L-Asp appears to have a genotoxic effect very close to the positive control in normal cells (p
- Published
- 2019