1. Pathological mechanisms of alcohol-induced hepatic portal hypertension in early stage fibrosis rat model
- Author
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Jian-zhao Niu, Xiao-Hua Tao, Yu Li, Ji-feng Wang, and Jian Li
- Subjects
Male ,medicine.medical_specialty ,Pathology ,Rats, Sprague-Dawley ,Type IV collagen ,chemistry.chemical_compound ,Fibrosis ,Internal medicine ,Hypertension, Portal ,Medicine ,Animals ,Vein ,Sirius Red ,Liver Diseases, Alcoholic ,business.industry ,Microcirculation ,Gastroenterology ,Radioimmunoassay ,General Medicine ,medicine.disease ,Rats ,Disease Models, Animal ,medicine.anatomical_structure ,Endocrinology ,Basic Research ,chemistry ,Immunohistochemistry ,Portal hypertension ,business ,Corn oil ,Liver Circulation - Abstract
AIM: To study the role of hepatic sinusoidal capillarization and perisinusoidal fibrosis in rats with alcohol-induced portal hypertension and to discuss the pathological mechanisms of alcohol-induced hepatic portal hypertension. METHODS: Fifty SD rats were divided into control group (n=20) and model group (n=30). Alcoholic liver fibrosis rat model was induced by intragastric infusion of a mixture containing alcohol, corn oil and pyrazole (1 000:250:3). Fifteen rats in each group were killed at wk 16. The diameter and pressure of portal vein were measured. Plasma hyaluronic acid (HA), type IV collagen (CoIV) and laminin (LN) were determined by radioimmunoassay. Liver tissue was fixed in formalin (10%) and 6-μm thick sections were routinely stained with Mallory and Sirius Red. Liver tissue was treated with rabbit polyclonal antibody against LN and ColIV. Hepatic non-parenchymal cells were isolated, total protein was extracted and separated by SDS-PAGE. MMP-2 and TIMP-1 protein expression was estimated by Western blotting. RESULTS: The diameter (2.207 ± 0.096 vs 1.528 ± 0.054 mm, P
- Published
- 2006