1. Effect of garlic on isoniazid and rifampicin-induced hepatic injury in rats
- Author
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Satya Vati Rana, Kim Vaiphei, Arbab Sikander, Kartar Singh, and R Pal
- Subjects
Necrosis ,Bilirubin ,Pharmacology ,medicine.disease_cause ,Lipid peroxidation ,chemistry.chemical_compound ,Isoniazid ,polycyclic compounds ,medicine ,Animals ,heterocyclic compounds ,Sulfhydryl Compounds ,Rats, Wistar ,Liver injury ,business.industry ,Gastroenterology ,food and beverages ,General Medicine ,Glutathione ,biochemical phenomena, metabolism, and nutrition ,bacterial infections and mycoses ,medicine.disease ,Rats ,Liver ,chemistry ,Biochemistry ,Lipid Peroxidation ,Rifampin ,medicine.symptom ,business ,Rapid Communication ,Rifampicin ,Oxidative stress ,medicine.drug - Abstract
To evaluate the hepatoprotective effect of garlic on liver injury induced by isoniazid (ZNH) and rifampicin (RIF).Wistar rats weighing 150-200 g were treated orally with 50 mg/kg of INH and RIF daily each for 28 d. For hepatoprotective studies, 0.25 g/kg per day of freshly prepared garlic homogenate was administered orally half an hour before the INH+RIF doses. Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) and bilirubin were estimated on d 0, 14, 21, and 28 in all the rats. Histological analysis was carried out to assess the injury to the liver. Lipid peroxidation (LPO) as a marker of oxidative stress and non-protein thiols (glutathione) for antioxidant levels were measured in liver homogenate.The treatment of rats with INH+RIF (50 mg/kg per day each) induced hepatotoxicity in all the treated animals as judged by elevated serum ALT, AST, and bilirubin levels, presence of focal hepatocytic necrosis (6/8) and portal triaditis (8/8). Garlic simultaneously administered at a dose of 0.25 g/kg per day prevented the induction of histopathological injuries in INH+RIF co-treated animals, except in 4 animals, which showed only moderate portal triaditis. The histological changes correlated with oxidative stress in INH+RIF treated animals. The group which received 0.25 g/kg per day garlic homogenate along with INH+RIF showed higher levels of glutathione (P0.05) and low levels of LPO (P0.05) as compared to INH+RIF treated group.Freshly prepared garlic homogenate protects against INH+RIF-induced liver injury in experimental animal model.
- Published
- 2006