1. [Magnetic beads adsorption-PCR detection method of four common foodborne pathogens in food].
- Author
-
Wang P, Hu Y, Tian X, Yang H, Zhao Y, and Chen Y
- Subjects
- Adsorption, Animals, Cronobacter sakazakii, Dairy Products, Escherichia coli, Listeria monocytogenes, Magnetic Phenomena, Meat, Milk, Staphylococcus aureus, Food Contamination, Food Microbiology, Polymerase Chain Reaction
- Abstract
Objective: To evaluate the adsorption capacity of magnetic beads for common foodborne pathogens., Methods: Escherichia coli, Staphylococcus aureus, Listeria monocytogenes and Cronobacter sakazakii were used as targets, the suitable conditions were obtained by optimization the incubation time, bead size, reaction temperature and so on. The adsorption efficiency of the magnetic beads in analog samples was also tested., Results: The optimum conditions were as follows: 2.0 - 3.0 microm diameter magnetic beads 50 microl, incubation for 60 min under 37 degrees C. In analog samples, the adsorption efficiency of the magnetic beads for Escherichia coli, Staphylococcus aureus, Listeria monocytogenes and Cronobacter sakazakii in juice, milk, duck meat were 50% - 84%, 46% - 66%, 54% - 74%, respectively., Conclusion: Magnetic beads enrichment technique has high adsorption efficiency, simple to operate, which is meet the requirement for PCR detection.
- Published
- 2014