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Start Over Author "T. Johnsson" Journal vox sanguinis
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3. Improved Technique for Detecting Australia Antigen by Immunoelectroosmophoresis

Author

T. Johnsson and Bengt Hansson

Subjects
Hepatitis B virus, Staining and Labeling, biology, Hematology, General Medicine, Hepatitis B Antigens, Hepatitis B, Ouchterlony double immunodiffusion, Molecular biology, Agar, Antigen, Methods, biology.protein, Humans, Antibody, Immunoelectrophoresis
Abstract

Comparison between the sensitivity in detecting Australia antigen of the Ouchterlony double diffusion method and the immunoelectroosmophoresis (IEOP) was made. The IEOP technique using pure agarose and staining the plates was 21 times more sensitive than the Ouchterlony method. In cases of hepatitis it was possible to detect Australia antigen for 50% longer time with IEOP than with the Ouchterlony method. The practical application of IEOP for detection of Australia antigen and antibodies is discussed.

Published
1971
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6. Comparative sensitivity o f immunoelectroosmophoresis and immune adherence hemagglutination tests for the detection of Australia antigen

Author

Bengt Hansson and T. Johnsson

Subjects
Hepatitis, Osmosis, Time Factors, Hemagglutination, business.industry, Immune adherence, Blood Donors, Hematology, General Medicine, Hemagglutination Tests, medicine.disease, Antibodies, Viral, Hepatitis B, Virology, Immune Adherence Reaction, Hemagglutination tests, Hepatitis B Antigens, Antigen, Evaluation Studies as Topic, Immunology, Methods, Medicine, Humans, business, Immunoelectrophoresis
Abstract

The immune adherence hemagglutination (IA) method was found to be about 30 times more sensitive than the immunoelectroosmophoresis (IEOP) technique in detecting Australia antigen. In cases of hepatitis, it was possible to detect Australia antigen with the IA method 5 days after the IEOP technique had become negative. One out of 7 Australia antigen-positive nonregistered blood donors at a certain time was negative with IEOP but positive with IA. The usefulness of the IA method compared to other techniques for detecting Australia antigen is discussed.

Published
1973

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