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Your search keyword '"Dmitry Gryadunov"' showing total 2 results
Start Over Author "Dmitry Gryadunov" Journal voprosy virusologii
2 results on '"Dmitry Gryadunov"'

1. [Biological microchip for identification of orthopoxviruses and agents causing the same clinical picture as in smallpox]

Author

Ai, Myznikova, Sa, Lapa, Dmitry Gryadunov, Da, Khodakov, As, Zasedatelev, and Vm, Mikhaĭlovich

Subjects
Diagnosis, Differential, Genes, Viral, Microchip Analytical Procedures, Humans, Orthopoxvirus, Poxviridae Infections, DNA Probes, Herpesviridae
Abstract

A method based on hybridization of simultaneously amplified gene fragments of orthopoxviruses and herpesviruses with oligonucleotide probes immobilized on a microarray has been developed. The method permits identification of 6 orthopoxvirus species and three types of herpesviruses, including Varicella zoster, within 6 hours.

Published
2007

2. [Identification of orthopoxvirus species using oligonucleotide micro-chips]

Author

Mv, Mikheev, Sa, Lapa, Sn, Shchelkunov, Ak, Chikova, Vm, Mikhaĭlovich, Sobolev AIu, Iv, Babkin, Dmitry Gryadunov, Ma, Bulavkina, Aa, Gus Kov, Eb, Sokunova, Gv, Kochneva, Vm, Blinov, Ls, Sandakhchiev, As, Zasedatelev, and Ad, Mirzabekov

Subjects
Viral Proteins, Base Sequence, Species Specificity, Gene Expression Profiling, DNA, Viral, Molecular Sequence Data, Animals, Humans, Orthopoxvirus, Oligonucleotide Probes, Sequence Alignment, Receptors, Tumor Necrosis Factor, Oligonucleotide Array Sequence Analysis
Abstract

A method for describing the Orthopoxviruses that are pathogenic both to man and animals is described in the article. The method is based on hybridization of a fluorescently labelled amplified DNA sample with oligonucleotides, which were immobilized in a microchip. Species-specific regions within the crmB gene encoding a viral analogue of the tumor necrosis factor receptor, i.e. an important gene determining the pathogenicity of the mentioned Orthopoxviruses type, were used as a target for identification. The identification procedure takes around 6 hours and does not demand any costly equipment (a portable fluorescent microscope can be used).

Published
2003

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