1. [An intensification of antiviral and interferon effects of ridostin (an interferon inducer) by cationic liposomes in vitro and in intranasal administration].
- Author
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Bulychev LE, Poryvaev VD, Ryzhikov AB, Karpyshev NN, Alekseeva AG, Goncharova EP, and Pliasunov IV
- Subjects
- Administration, Intranasal, Animals, Antiviral Agents administration & dosage, Brain drug effects, Brain immunology, Cardiovirus Infections immunology, Cell Line, Cytopathogenic Effect, Viral, Drug Delivery Systems, Interferon Inducers administration & dosage, Interferons biosynthesis, Liposomes administration & dosage, Liposomes chemistry, Lung drug effects, Lung immunology, Mice, Mice, Inbred BALB C, Olfactory Mucosa drug effects, Olfactory Mucosa immunology, RNA, Double-Stranded administration & dosage, RNA, Fungal administration & dosage, Antiviral Agents pharmacology, Cardiovirus Infections drug therapy, Encephalomyocarditis virus drug effects, Interferon Inducers pharmacology, Liposomes pharmacology, RNA, Double-Stranded pharmacology, RNA, Fungal pharmacology
- Abstract
Combined application of ridostine with catonic liposomes was shown to essentially enhance the interferon-inducing and antiviral activity of the former in experiments with cell cultures L-929, which is apparently related with an improved efficiency of intracellular delivery of dsRNA. A comparative study demonstrated that ridostine, when combined with liposomes, is needed by 10(3)-10(4) times less as when it is used alone. A pretreatment of the cellular monolayer by cationic liposomes contributes also to enhancing the activity of ridostine, which can be explained by an enhanced permeability of cells for dsRNA holding on-for as long as 30 minutes after the removal of liposomes from the liquid culture. A separate successive administration of, first, liposomes and, then, of ridostine in BALB/c mice (20 mg/kg) leads to a more intensified induction of interferon in the upper respiratory tract tissues as compared with the administration of ridostine alone.
- Published
- 2003