163 results
Search Results
2. [Determination of antigens to viruses and Rickettsia with the aid of antigens fixed on filter paper].
- Author
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NEZLIN RS, GURVICH AE, and TOVARNITSKII VI
- Subjects
- Filtration, Rickettsia, Viruses immunology
- Published
- 1959
3. [Physicochemical characteristics and macromolecular organization of bacteriophage FI-5 (author's transl)].
- Author
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Andriashvili IA, Petrovskiĭ GV, Kvachiadze LI, and Adamiia RSh
- Subjects
- Bacteriophages isolation & purification, Base Sequence, Centrifugation, Density Gradient, Chemical Phenomena, Chemistry, Physical, Chromatography, Paper, DNA, Viral analysis, DNA, Viral isolation & purification, Electrophoresis, Polyacrylamide Gel, Genes, Macromolecular Substances, Microscopy, Electron, Molecular Conformation, Nitrogen, Spectrophotometry, Ultracentrifugation, Viral Proteins analysis, Viscosity, Bacteriophages analysis, Escherichia coli
- Abstract
Physico-chemical parameters and features of macromolecular orgnization of FI-5 phage were studied. This virus was shown to contain a molecule of double-strander DNA with the standard set of nitrous bases (37.8 mol% GC). The molecule of this DNA in situ is characterized by partial disorder of the second structure. Phage virions contain about 47% of DNA and 53% of protein. The genome of the phage is represented by a DNA molecule with molecular weight 65X10(6) daltons and is capable of coding for a least 15 different proteins.
- Published
- 1975
4. [Properties of individual mononucleotides of DNA of phage DDVII (Shigella dysenteriae flexneri)].
- Author
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Trushinskaia GN, Nikol'skaia II, and Tikhonenko TI
- Subjects
- Chromatography, Ion Exchange, Chromatography, Paper, Spectrum Analysis, Bacteriophages, DNA, Viral, Nucleotides, Shigella dysenteriae
- Published
- 1972
5. [Viruses and bats: interdisciplinary issues].
- Author
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Botvinkin AD
- Subjects
- Animals, Humans, COVID-19 epidemiology, COVID-19 transmission, Chiroptera virology, Paramyxoviridae, Paramyxoviridae Infections epidemiology, Paramyxoviridae Infections transmission, Rhabdoviridae, Rhabdoviridae Infections epidemiology, Rhabdoviridae Infections transmission, SARS-CoV-2, Zoonoses epidemiology, Zoonoses virology
- Abstract
The virologists' attention to bats (Сhiroptera) changed in the late 20th century as the concept of emerging infections grew in popularity. Since the beginning of the COVID-19 pandemic, the number of publications on bat viruses has increased profoundly.History of the problem; biodiversity of Chiroptera and related viruses; medical and veterinary significance of some viral genera and subgenera (Lyssavirus, Henipavirus, Marburgvirus, Ebolavirus, Sarbecovirus, Merbecovirus), as well as problems of bat protection, are addressed in a concise form. Literature search was carried out in electronic databases, mainly for the period of 2000-2021. Publications in Russian that are poorly represented in English-language reviews are also included. The purpose of the review is to substantiate the importance of an interdisciplinary approach in the context of increased interest in the study of viral infections in bats. This review was written for researchers who have not previously dealt with this problem.Since the beginning of this century, the number of known virus species associated with bats has increased by an order of magnitude (>200). The families Rhabdoviridae, Coronaviridae, Paramyxoviridae are in the first ranks according to the number of findings, and the highest diversity of viruses has been established for the families Vespertilionidae, Pteropodidae, Molossidae. Interdisciplinary cooperation positively influences the efficiency, biological safety and practical significance of the ongoing research. The best results were achieved by multidisciplinary teams with good cross-training in several specialties. Many papers emphasize the need to balance health and conservation interests.The analysis of scientific publications indicates a change in research approaches in this area: from collecting individual facts within the framework of narrow specialties to a comprehensive assessment of new knowledge from ecological, evolutionary and socio-economic positions. Results of the research emphasize the need to maintain complex approaches addressing public health needs and environmental protection. The importance of bat-borne viral infections determines the necessity for correction and interdepartmental coordination of scientific research and surveillance of wildlife zoonoses in the Russian Federation.
- Published
- 2021
- Full Text
- View/download PDF
6. [Susceptibility of animal species to experimental SARS-CoV-2 ( Coronaviridae: Coronavirinae: Betacoronavirus; Sarbecovirus ) infection].
- Author
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Petrova NV, Ganina KK, and Tarasov SA
- Subjects
- Animals, COVID-19 epidemiology, COVID-19 pathology, Cricetinae, Humans, Mice, Pandemics, Species Specificity, Strepsirhini, COVID-19 metabolism, Disease Models, Animal, SARS-CoV-2 metabolism
- Abstract
Due to the new coronavirus infection pandemic, the global scientific community has been forced to change the direction of the most research, focusing on vaccine development as well as the search for new antiviral drugs to treat COVID-19. The choice of experimental models, timeframe and approaches for evaluating drugs and vaccines under development is crucial for the development of effective measures to prevent and control this disease.The purpose of this review was to summarize the relevant data concerning the susceptibility of laboratory animals to SARS-CoV-2. This paper describes the most virus-susceptible animal species that can be used to reproduce coronavirus infection, stressing the main advantages and disadvantages of each of them.According to the latest data, small rodents (Rodentia) and non-human primates (Strepsirrhini) are commonly used in the scientific community to model coronavirus infection. The viral load in the upper and lower parts of the respiratory system, clinical symptoms of infection (weight loss, body temperature and general health status), pathomorphological picture in target organs and the production of antibodies after infection are considered to the main markers of pathology. Despite the vast amount of data, none of the described models of SARS-CoV-2 infection may be considered a gold standard, since they do not reproduce all spectrum of morphological and pathogenetic mechanisms of infection, and do not fully reflect the clinical picture observed in patients in human population.Based on the analyzed literature data, we suppose that Syrian hamster (Mesocricetus auratus) and mice (Muridae) expressing the angiotensin converting enzyme receptor 2 (ACE2) are the most suitable animal species for their use in experiments with SARS-CoV-2 infection. The development of neutralizing antibodies makes it possible to evaluate the efficacy of vaccines, while the course and severity of symptoms infection makes the use of mice and hamsters especially popular for screening pharmacological substances with antiviral mechanism of action, when their administration can prevent or slow the disease progression.
- Published
- 2021
- Full Text
- View/download PDF
7. [Development of the drug oncolytic immunotherapy based on vaccinia viruses (Vaccinia virus, Orthopoxvirus, Chordopoxvirinae, Poxviridae) against breast cancer.]
- Author
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Bauer TV, Tregubchak TV, Maksyutov AZ, Kolosova IV, Maksyutov RA, and Gavrilova EV
- Subjects
- Animals, Breast Neoplasms genetics, Breast Neoplasms virology, Cell Line, Tumor, Female, Genetic Vectors genetics, Genetic Vectors pharmacology, Humans, Immunotherapy, Orthopoxvirus genetics, Poxviridae genetics, Virus Replication immunology, Breast Neoplasms therapy, Oncolytic Viruses genetics, Vaccinia virus genetics, Virus Replication genetics
- Abstract
Introduction: Currently, new directions in cancer therapy are actively developing, one of which is oncolytic immunotherapy. This approach would be to use of viruses as cancer specific cytolytic agents capable of stimulating both the tumor-specific and non-specific immune response. The objective paper was obtain a recombinant vaccinia virus containing genes encoding immunostimulating molecules and study oncolytic and immunostimulating properties of recombinant virus., Material and Methods: MTT test, ELISA, methods of transient dominant selection., Results: The recombinant vaccinia virus (L-IVP_oncoB) were obtained with deletion of the gene encoding thymidine kinase and had an integrated gene encoding GM-CSF. Also the virus have deletion of the gene encoding viral growth factor and integrated genes encoding synthetic tumor-specific polyepitopic immunogens. It was shown that the modifications made to the viral genome did not affect the growth characteristics of the virus when cultured on CV-1 and 4647 cell cultures, and the cytopathogenic efficacy of the virus was determined in relation to cancer cultures of cells of various genesis. In in vivo experiment, it was revealed that the polyepitopic construct in the genome L-IVP_oncoB is able to initiate a change in the profile of cytokines., Discussion: The obtained data characterized L-IVP_oncoB as a promising cytopathogenic and immunostimulating agent and showed the need for further study of its properties as means of oncolytic immunotherapy., Conclusion: The basic experiments on the evaluation of the biological properties of the obtained L-IVP_oncoB, which are necessary for the characterization of the oncolytic virus, have been carried out., Competing Interests: The authors declare no conflict of interest.
- Published
- 2020
- Full Text
- View/download PDF
8. [Lujo hemorrhagic fever].
- Author
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Sizikova TE, Lebedev VN, Syromyatnikova SI, and Borisevich SV
- Subjects
- Arenaviridae Infections, Humans, South Africa, Hemorrhagic Fevers, Viral, Lujo virus, Phylogeny
- Abstract
Lujo hemorrhagic fever (LHF) is a viral disease accompanied with fever, headache, vomiting, diarrhea, arthralgia, myalgia and numerous signs of hemorrhagic syndrome. LHF causes a clinical syndrome remarkably similar to Lassa hemorrhagic fever. The first case of LHF occurred in Johannesburg, South Africa, in 2008. There was a secondary transmission from the index patient to four healthcare workers. Four of the five patients died. The etiologic agent of LHF is Lujo virus (LUJV) belonging to Arenavirus genus of the Arenaviridae Family. Virus Lujo is the second pathogenic arenavirus, after Lassa virus, to be recognized in Africa during the last 40 years. Data about epidemiology, clinical characteristics and diagnostics of LHF, properties of Lujo virus (according to phylogenetic analysis), and recommended precautions for preventing secondary transmission are considered in this paper., Competing Interests: The authors declare no conflict of interest.
- Published
- 2017
- Full Text
- View/download PDF
9. [Molecular genetic characterization of the Gissar virus (GSRV) (Bunyaviridae, Phlebovirus, Uukuniemi group) isolated from the ticks Argas reflexus Fabricius, 1794 (Argasidae) collected in dovecote in Tajikistan].
- Author
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L'vov DK, Al'khovskiĭ SV, Shchelkanov MIu, Shchetinin AM, Deriabin PG, Gitel'man AK, Aristova VA, and Botikov AG
- Subjects
- Amino Acid Sequence, Animals, Argasidae virology, Birds virology, Bunyaviridae pathogenicity, Bunyaviridae Infections genetics, High-Throughput Nucleotide Sequencing, Molecular Sequence Data, Tajikistan, Bunyaviridae genetics, Bunyaviridae Infections virology, Genome, Viral, Phylogeny
- Abstract
The Gissar virus (GSRV) was originally isolated from the ticks Argas reflexus, Fabricius, 1794 collected in a dovecote of Gissar village in Tajikistan (38 degrees 40' N, 68 degrees 40' E). Using electron microscopy, GSRV was classified to Bunyaviridae without referring to genus due to the absence of the antigenic relation with known bunyaviruses. In the present paper genome of GSRV was sequenced (MiSeq, Illumina). Molecular genetics and phylogenetic analysis showed. GSRV has a high level of homology with the Grand Arbaud Virus (GAV) (94% for nucleocapsid protein, 87.5% for RdRp, and 82% for the envelope proteins GnGc) isolated from the ticks A. Reflexus in a dovecote in France. GSRV and GAV have a narrow ecological niche associated with the icks A. Reflexus and birds (predominantly Columbidae). According to the conducted study, GSRV is classified as the topotypic for Central Asia variant of GAV, Uukuniemi group, genuses of the Phlebovirus (Bunyaviridae) (ID GenBank KJ425423, KJ425424, KJ425425).
- Published
- 2014
10. [Genetic characterization of the Syr-Darya valley fever virus (SDVFV) (Picornaviridae, Cardiovirus) isolated from the blood of the patients and ticks Hyalomma as. asiaticum (Hyalomminae), Dermacentor daghestanicus (Rhipicephalinae) (Ixodidae) and Ornithodoros coniceps (Argasidae) in Kazakhstan and Turkmenistan].
- Author
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L'vov DK, Al'khovskiĭ SV, Shchelkanov MIu, Shchetinin AM, Deriabin PG, Gitel'man AK, Aristova VA, and Botikov AG
- Subjects
- Amino Acid Sequence, Animals, Argasidae virology, Birds virology, Humans, Ixodidae virology, Kazakhstan, Metagenome, Molecular Sequence Data, Picornaviridae pathogenicity, Picornaviridae Infections genetics, Turkmenistan, Genome, Viral, Phylogeny, Picornaviridae genetics, Picornaviridae Infections virology
- Abstract
The Syr-Darya valley fever virus (SDVFV) was originally isolated from the blood of the patient with fever in the Kyzylorda province, Kazakhstan, in July 1973 and was classified to the Cardiovirus genus (fam. Picornaviridae). Later, SDVFV was isolated from the ticks Hyalomma as. asiaticum Schulze et Schlottke, 1929 (Hyalomminae) (1 strain) and Dermacentor daghestanicus Olenev, 1929 (Rhipicephalinae) (7 strains), collected in the floodplains of the Syr-Darya river and the Ili river. In this paper, complet genome of the SDVFV (strain LEIV-Tur2833) was sequenced using the next-generation sequencing approach (GenBank ID: KJ191558). It was demonstrated that, phylogenetically, the SDVFV is closely related closest to the Theiler's murine encephalomyelitis virus (TMEV) and Vilyuisk human encephalomyelitis virus (VMEV). The similarity of the SDVFV with VHEV and TMEV based on P1 region of the polyprotein-precursor (structural proteins VP1-VP4), reaches 75% and 91% for nucleotide sequences and 80% and 93% for putative amino acid sequences, respectively. For nonstructural proteins regions P2 (2A-2C) and P3 (3A-3D) similarity of SDVFV with TMEV and VHEV is 96%-98%.
- Published
- 2014
11. [A new principle in the selection of vaccinal rabies virus based on quantitation of the expression of G protein, a major immunogen of rabies virus].
- Author
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Gribencha SV, Losich MA, Gribencha LF, and Nepoklonova IV
- Subjects
- Animals, Cattle, Gene Expression Regulation, Viral immunology, Glycoproteins metabolism, Humans, Mice, Rats, Glycoproteins immunology, Rabies prevention & control, Rabies virology, Rabies Vaccines genetics, Rabies Vaccines immunology, Rabies Vaccines metabolism, Rabies virus immunology, Rabies virus metabolism, Rabies virus pathogenicity
- Abstract
The paper describes a new principle of the selection of the rabies virus vaccine strain ERA-CB20M based on quantitation of the expression of glycoprotein, a major immunogen of rabies virus. There is a correlation between the level of glycoprotein expression and that of vaccine virus attenuation. The application of the new principle for vaccine virus selection will permit a safer and more immunogenic rabies vaccine to be prepared.
- Published
- 2012
12. [A new unique HIV-1 recombinant form detected in Belarus].
- Author
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Eremin VF, Gasich EL, and Sosinovich SV
- Subjects
- HIV-1 classification, Humans, Phylogeny, Reassortant Viruses genetics, Republic of Belarus, Sequence Analysis, DNA, env Gene Products, Human Immunodeficiency Virus genetics, pol Gene Products, Human Immunodeficiency Virus genetics, HIV Infections genetics, HIV-1 genetics, Recombination, Genetic genetics, gag Gene Products, Human Immunodeficiency Virus genetics
- Abstract
Republican Research-and-Practical Center for Epidemiology and Microbiology, Ministry of Health of Belarus, Minsk The paper presents data on the molecular genetic characteristics of a new HIV-1 recombinant form. The study has shown that the virus is referred to as HIV-1 subtype B in terms of the gag gene and HIV-1 subtype A in terms of the pol and env genes. At the same time the new isolate is closer, in terms of the gag gene, to the HIV-1 DQ207943 strain isolated in Georgia, in terms of the pol gene, to the HIV-1 AF413987.1 strain isolated in Ukraine and, in terms of the env gene to the HIV-1 AY500393 strain isolated in Russia. Thus, the described new HIV-1 recombinant form has the following structure: BgagApolAenv. The gag, pol, and env gene sequences from the new unique HIV-1 recombinant form have been registered in the international database EMBL/Genbank/DDBJ under accession numbers FR775442.1, FN995656.1, and FR775443.1.
- Published
- 2012
13. [Development of methodology for predictably significant evaluation of the protective efficacy of antiviral agents].
- Author
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Markin VA
- Subjects
- Animals, Disease Models, Animal, Encephalomyelitis, Venezuelan Equine drug therapy, Haplorhini, Hemorrhagic Fever, Ebola drug therapy, Humans, Treatment Outcome, Antiviral Agents pharmacology, Drug Evaluation, Preclinical methods, Virus Diseases drug therapy
- Abstract
The paper provides a theoretical analysis for determining whether the antiviral nonspecific drugs being tested are promising to solve biosafety problems in the treatment of exotic viral infections. The essence of the proposed concept of evaluation of protective effectiveness is to analyze the effect of a test drug on the pathogenesis of experimental infection from the fact that it is effective in adequately eliminating the animal-simulated leading syndrome of human disease. The given approaches to using adequacy criteria to select the species of animals meeting the goals of tests in terms of pathogenetic and pharmacological parameters determine a new methodology for evaluating the efficacy of protective agents. Basic requirements for a testing procedure are presented. The prognostic value of evaluation of the protective efficacy of antiviral agents for man will depend on the approximation of the pathogenetic features and external manifestation of disease in the selected animal species to human Infection. The paper also covers the comparative characteristics of the course of Ebola fever and Venezuelan equine encephalomyelitis in man and some species of monkey.
- Published
- 2012
14. [Interaction of influenza A and B viruses with nanodiamond-based sorbents].
- Author
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Ivanova VT, Ivanova MV, Burtseva EI, Garina EO, Trushakova SV, Shevchenko ES, Manykin AA, Isakova AA, Korzhenevskiĭ AP, and Spitsyn BV
- Subjects
- Adsorption, DNA, Viral isolation & purification, Humans, Influenza A virus isolation & purification, Influenza B virus isolation & purification, Influenza, Human prevention & control, Temperature, Time Factors, DNA, Viral chemistry, Influenza A virus chemistry, Influenza B virus chemistry, Influenza, Human virology, Nanodiamonds chemistry
- Abstract
The paper presents data on the sorption of influenza A(H1N1), A(H1N1)v, A(H3N2) viruses, cDNA of A(H1N1)v and B viruses on nanodiamonds and furnace charge. The sorption of viruses occurred in different solutions at 4-37 degrees C during 10-20 min. The rate of sorption varied with the concentration of a sorbent in the solution and its structure, but did not with the antigenic formula of viruses or temperature. The sorption capacity of furnace charge towards influenza A and B viruses was higher than that of nanodiamonds. Nonviral proteins (bovine serum albumin and influenza virus antibodies) were found to be bound by both sorbents. Viral desorption did not take place in physiological solution at 4 and 22 degrees C for 48 hours.
- Published
- 2012
15. [The specific features of the cocirculation of influenza viruses in the 2010-2011 postpandemic period according to the results of activities of the D. I. Ivanovsky Research Institute of Virology, Ministry of Health and Social Development of Russia].
- Author
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Burtseva EI, L'vov DK, Shchelkanov MIu, Kolobukhina LV, Prilipov AG, Al'khovskiĭ SV, Lavrishcheva VV, Shevchenko ES, Fediakina IT, Ivanova VT, Beliakova NV, Proshina ES, Abramov DD, Trushakova SV, Merkulova LN, Vartanian RV, Kisteneva LB, Samokhvalov EI, Oskerko TA, Feodoritova EL, Siluianova ÉV, Mukasheva EA, Beliaev AL, Malikov VE, and Malyshev NA
- Subjects
- Academies and Institutes, Adenoviridae drug effects, Adenoviridae physiology, Adenoviridae Infections drug therapy, Adenoviridae Infections virology, Adolescent, Adult, Antiviral Agents administration & dosage, Antiviral Agents therapeutic use, Child, Child, Preschool, Coinfection, Drug Resistance, Viral, Humans, Influenza A Virus, H1N1 Subtype drug effects, Influenza A Virus, H1N1 Subtype physiology, Influenza A Virus, H3N2 Subtype drug effects, Influenza A Virus, H3N2 Subtype physiology, Influenza B virus, Influenza, Human drug therapy, Influenza, Human virology, Oseltamivir administration & dosage, Oseltamivir therapeutic use, Respirovirus drug effects, Respirovirus physiology, Respirovirus Infections drug therapy, Respirovirus Infections virology, Rimantadine administration & dosage, Rimantadine therapeutic use, Russia epidemiology, Seasons, Adenoviridae Infections epidemiology, Influenza, Human epidemiology, Pandemics, Respirovirus Infections epidemiology
- Abstract
The paper gives the results of monitoring the circulation of influenza viruses in the 2010-2011 season, that covers the second year of circulation of pandemic A(H1N1)v virus strains, and their interaction with seasonal A (H3N2) and B strains. Unlike the previous season, the beginning of an increase in morbidity was recorded in January 2011; its peak in the most of contiguous areas was noted at 5-7 weeks of 2011, with its further decline to threshold levels at week 11 of 2011. Preschool and school children were most involved in the epidemic process. Three influenza virus strains (A(H1N1)v, A(H3N2), and B) were found to circulate. Differences were found in the level of participation of the isolated strains in individual areas of the Russian Federation. Detailed typing of the isolated strains determined the compliance of the vast majority of them with vaccine viruses. The pandemic influenza A(H1N1)v virus strains retained their susceptibility to oseltamivir and were resistant to rimantadine. The participation of non-influenza acute respiratory viral infection pathogens was estimated as follows: 11.9% for parainfluenza viruses, 5.9% for adenoviruses, and 3.5% for PC viruses, and 0.7% for pneumonia Mycoplasma, which was comparable with the previous epidemic seasons.
- Published
- 2012
16. [Evaluation of the efficiency of differential diagnosis of influenza by multiplex real-time polymerase chain reaction].
- Author
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Lobodanov SA, Nikonova AA, Faĭzuloev EB, Trushakova SV, Zabiiaka IuI, Kalinkina MA, Ivanova VT, Shevchenko ES, Zverev VV, and Burtseva EI
- Subjects
- Animals, Cell Culture Techniques, Cell Line, Diagnosis, Differential, Dogs, Female, Humans, Influenza A virus isolation & purification, Influenza B virus isolation & purification, Influenza, Human virology, Male, Nasopharynx virology, Real-Time Polymerase Chain Reaction, Sensitivity and Specificity, Influenza A virus genetics, Influenza B virus genetics, Influenza, Human diagnosis
- Abstract
The paper gives the results of a comparative analysis of the detection of influenza viruses in clinical samples, by using multiplex real-time polymerase chain reaction (RT-PCR) and by virus isolation in MDCK cell cultures. The investigation employed 267 nasopharyngeal swab specimens obtained from patients with influenza symptoms during two epidemic seasons (2008-2009 and 2009-2010). Influenza viruses were found in 104 samples (48 with influenza A virus (IAV) and 56 with influenza B virus (IBV)) by multiplex RT-RCR and in 84 samples (35 with IAV and 49 with IBV) by a cultural technique. The results of detection of influenza viruses by the two methods showed 89.4% agreement. The diagnostic sensitivity of multiplex RT-PCR testing a panel of the clinical samples in question was estimated to be 94.3% for IAV and 95.9% for IBV. The diagnostic sensitivity of multiplex RT-PCR in virus detection was demonstrated to be not only highly competitive with virus isolation, but also superior to the latter.
- Published
- 2012
17. [Preclinical studies of live intranasal H5N1 influenza vaccine with the deleted HS1 gene].
- Author
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Romanovskaia-Roman'ko EA, Ferko B, Vyshemirskiĭ OI, Romanova IuR, Krenn B, Muster T, Grudinin MP, Lapin BA, Egorov AIu, and Kiselev OI
- Subjects
- Administration, Intranasal, Animals, Chlorocebus aethiops, Cross Protection immunology, Drug Evaluation, Preclinical, Humans, Influenza A Virus, H5N1 Subtype immunology, Influenza Vaccines genetics, Influenza Vaccines immunology, Influenza, Human genetics, Influenza, Human immunology, Interferons metabolism, Macaca fascicularis, Mice, Orthomyxoviridae Infections genetics, Orthomyxoviridae Infections immunology, Reverse Genetics methods, Vaccines, Attenuated genetics, Vaccines, Attenuated immunology, Vero Cells, Viral Nonstructural Proteins immunology, Influenza A Virus, H5N1 Subtype genetics, Influenza Vaccines therapeutic use, Influenza, Human prevention & control, Orthomyxoviridae Infections prevention & control, Vaccines, Attenuated therapeutic use, Viral Nonstructural Proteins genetics
- Abstract
The paper gives the results of evaluating the efficiency of deINS1 pandemic H5N1 vaccine candidate VN1203delNS1 which was constructed by reverse genetics on the basis of influenza virus strain A/Vietnam/1203/04. The safety, immunogenicity and cross-protection of the vaccine strain against different H5N1 virus clades were demonstrated in mouse and macaque models. The results showed the possibility of designing a new-generation replication-deficient intranasal influenza vaccine, by applying an approach to deleting the NS1 pathogenicity factor, an antagonist of the interferon system.
- Published
- 2011
18. [Comparative estimation of the indicators of interferon, immune, and cytokine states in the comprehensive study of patients with herpesvirus infections].
- Author
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Mel'nichenko AV, Mezentseva MV, Shapoval IM, Tregubova MI, Podcherniaeva RIa, and L'vov ND
- Subjects
- Adolescent, Adult, Aged, Female, Herpesviridae Infections immunology, Herpesviridae Infections metabolism, Herpesviridae Infections therapy, Humans, Male, Middle Aged, Treatment Outcome, Antibodies, Viral metabolism, Herpesviridae immunology, Interferon-alpha metabolism, Interferon-alpha therapeutic use, Interferon-gamma metabolism, Interferon-gamma therapeutic use, Interleukins metabolism
- Abstract
The paper provides the data of a comparative analysis of the indicators of immune and interferon states and cytokine profile and the results of virological studies in patients with different (acute and chronic) forms of mixed herpesvirus infection (with virus simplex herpes types 1 and 2, Epstein-Barr virus, human herpesvirus type 6, and others). Pronounced changes were found in immune responses in such patients. There were decreases in IFN-alpha and IFN-gamma values in 36 and 13%, respectively; 51% of the subjects showed a reduction in both IFN-alpha and IFN-gamma along with the high titers of antibodies to viruses of the Herpesviridae family and their infectious activity. There were changes in the cytokine profile, activation of IFN-alpha, IL-2, IL-8, IL-10, and IL-18 gene expression, and suppression of IL-2 gene transcription in the majority of the patients. Determination of IFN susceptibility revealed that 86% of the subjects responded to IFN-alpha therapy and only 11% of cases did to IFN-gamma one.
- Published
- 2011
19. [A prognostic model of the effect of the antiviral drug Relenza on the epidemic of A(H1N1)/2009 influenza].
- Author
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Boev BV and Ershov IF
- Subjects
- Adolescent, Adult, Algorithms, Antiviral Agents pharmacology, Antiviral Agents therapeutic use, Child, Humans, Influenza A Virus, H1N1 Subtype drug effects, Middle Aged, Russia epidemiology, Zanamivir pharmacology, Computer Simulation, Disease Outbreaks statistics & numerical data, Influenza, Human drug therapy, Influenza, Human epidemiology, Influenza, Human prevention & control, Models, Theoretical, Zanamivir therapeutic use
- Abstract
The paper gives the results of calculation-theoretical studies estimating the effect of the etiotropic agent Relenza (in preventing influenza in its susceptible patients an in treating patients ill with influenza) on the epidemic of pandemic A(H1N1)/2009 influenza in a large city of Russia. The values of its effect (the number of prevented cases of influenza and that of prevented deaths from its complications) have been calculated on a computer, by applying a modified PSEEI2RF influenza epidemic model (a Russian Baroyan-Rvachev model) with the A(H1N1)/2009 influenza pathogen that dominated in the 2009-2010 season in many countries of the world. Predictive estimates of the action of Relenza on the epidemic of A(H1N1)/2009 influenza have been obtained for 5 scenarios while implementing measures to treat patients with the illness and to prevent its susceptible patients in a large city with a population of one million. In conclusion, there are results of predicting the number of prevented A(H1N1)/2009 influenza cases and damage estimates for 6 cities of Russia due to the massive use of the antiviral drug Relenza.
- Published
- 2011
20. [Detection of bovine respiratory syncytial virus by RT-PCR].
- Author
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Glotov AG, Glotova TI, and Stroganova IIa
- Subjects
- Animals, Cattle, Cattle Diseases virology, Nasal Mucosa virology, RNA, Viral analysis, Respiratory Syncytial Virus, Bovine genetics, Sensitivity and Specificity, Siberia, Cattle Diseases diagnosis, Respiratory Syncytial Virus Infections diagnosis, Respiratory Syncytial Virus Infections veterinary, Respiratory Syncytial Virus, Bovine isolation & purification, Reverse Transcriptase Polymerase Chain Reaction methods, Reverse Transcriptase Polymerase Chain Reaction veterinary
- Abstract
The paper presents the results of studying the diagnostic efficiency of RT-PCR for the detection of respiratory syncytial virus in cattle of different ages. Glycoprotein F gene sequences were used as a target for amplification. The sensitivity of the reaction was 10 TCD50/ml and the virus detection rate in biomaterials averaged 19%. samples. That in RT-PCR correlated with the presence of clinical signs in sick animals.
- Published
- 2011
21. [Antiviral humanized and human monoclonal antibodies].
- Author
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Lashkevich VA
- Subjects
- Antibodies, Viral therapeutic use, Cytomegalovirus Infections therapy, Epstein-Barr Virus Infections therapy, HIV Infections drug therapy, Hepatitis B prevention & control, Hepatitis B therapy, Hepatitis C drug therapy, Humans, Immunization, Passive methods, Palivizumab, Respiratory Syncytial Virus Infections therapy, Virus Diseases prevention & control, Virus Diseases therapy, Antibodies, Monoclonal, Humanized biosynthesis, Antibodies, Monoclonal, Humanized immunology, Antibodies, Monoclonal, Humanized therapeutic use, Antibodies, Viral immunology, Antiviral Agents therapeutic use, Drug Industry trends, Viruses immunology
- Abstract
The paper considers the characteristics of monoclonal antibodies, methods for their experimental preparation, problems of their production, and possibilities of their use for the emergency prevention of viral infections and for the treatment of chronic diseases caused by human immunodeficiency virus, hepatitis B and C viruses, and herpes viruses. The future of experimentally produced or clinically trialed monoclonal antibodies is mainly determined by commercial considerations. It is possible that simplification of industrial production technologies and a reduction in the cost of evidence-based methods for evaluation of clinical effectiveness will allow monoclonal antibodies to be extensively used for the prevention and treatment of viral infections.
- Published
- 2011
22. [Viral sorption on polyaniline, carbon nanotubes and their based nanocomposites].
- Author
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Ivanova VT, Kurochkina IaE, Ivanov VF, Il'ina MV, Trushakova SV, Shevchenko ES, Burtseva EI, Simakova AA, Manykin AA, Nosik NN, Shneider MM, Timofeeva AV, and Sapurina IIu
- Subjects
- Adsorption, Aniline Compounds chemistry, Animals, Birds, Filtration instrumentation, Hemagglutinin Glycoproteins, Influenza Virus chemistry, Humans, Influenza in Birds virology, Influenza, Human virology, Moscow, Temperature, Bacteriophages chemistry, Influenza A virus chemistry, Nanostructures chemistry, Poliovirus chemistry, Reassortant Viruses chemistry
- Abstract
The paper gives data on the sorption of influenza virus pandemic strain A/IIV-Moscow/01/2009 (H1N1)swl, avian influenza viruses with A/H5 and A/H7 hemagglutinin, poliomyelitis virus, and T4-D bacteriophage on polyaniline sorbents, carbon nanotubes, and their based nanocomposites. The sorption of viruses occurred in different solutions at 4-37 degrees C during 15 min or more. The rate of viral sorption depended on the structure of sorbents.
- Published
- 2011
23. [Retherapy with lamivudine in hbeag-negative chronic hepatitis B patients unresponsive to interferon/peginterferon treatment].
- Author
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Fedorchenko SV, Martynovich TL, Ianchenko VI, Klimenko ZhB, and Liashok OV
- Subjects
- Adult, Antiviral Agents administration & dosage, Child, Preschool, DNA, Viral analysis, Female, Hepatitis B e Antigens analysis, Hepatitis B virus genetics, Hepatitis B, Chronic virology, Humans, Interferon alpha-2, Interferon-alpha pharmacology, Lamivudine administration & dosage, Male, Middle Aged, Mutation, Polyethylene Glycols pharmacology, Recombinant Proteins, Russia, Treatment Outcome, Viremia, Virus Replication drug effects, Antiviral Agents therapeutic use, Hepatitis B virus drug effects, Hepatitis B, Chronic drug therapy, Lamivudine therapeutic use
- Abstract
The paper gives data on retherapy with lamivudine in seven HBeAg-negative chronic hepatitis B patients previously unresponsive to interferon-alpha and peginterferon-alpha2b therapy (6 unresponsive patients and 1 with recurrence). Prior to lamivudine therapy, hepatic biopsy and HBV genotyping were carried out and the baseline level of viremia and the presence of YMDD mutations were determined in all the patients. Its therapeutic efficiency was based on viremia level and AIAT activity at 6, 12, 18, and 24 weeks of treatment. Six of the 7 patients, including 2 receiving peginterferon-a2a, achieved a virological response. Trends in viremia within the first 12 weeks of treatment were a major factor to assess the virological response. Positive predictors during retreatment with lamivudine are discussed.
- Published
- 2011
24. [Pandemic influenza 2009 in Russia. Characteristics of the isolation and biological properties of viruses].
- Author
-
Danilenko DM, Konovalova NI, Eropkin MY, Gudkova TM, Grigoryeva VA, Ivanova AV, Shchekanova SM, Smirnova TD, and Kiselev OI
- Subjects
- Animals, Antibodies, Viral, Antigenic Variation, Antigens, Viral immunology, Antigens, Viral isolation & purification, Cell Culture Techniques, Cell Line, Chick Embryo, Dogs, Hemagglutination Inhibition Tests, Hemagglutination, Viral immunology, Humans, Organ Specificity immunology, Pandemics, Rats, Russia epidemiology, Swine, Antigens, Viral analysis, Influenza A Virus, H1N1 Subtype growth & development, Influenza A Virus, H1N1 Subtype immunology, Influenza A Virus, H1N1 Subtype isolation & purification, Influenza A Virus, H1N1 Subtype pathogenicity, Influenza, Human immunology, Influenza, Human virology
- Abstract
Research Institute of Influenza, Ministry of Health and Social Development of Russia, Saint Petersburg The characteristics of the isolation of pandemic influenza A(H1N1)v viruses were studied on chick embryos (CE) and MDCK cell culture. The materials (nasal swabs and autopsies) were collected in different regions Russia in the period from 20 July to 30 December 2009. The paper gives the data of the antigenic analysis of isolates, their capacity to multiply in different species-specific and tissue cell cultures. The viruses isolated on CE were shown to have higher hemagglutination titers and to be more stable. Isolation from the autopsies was effective only on CE. All the test cell lines other than MDCK were insensitive to the isolated pandemic influenza strains. The antigenic analysis showed no significant antigenic drift of the viruses isolated during the first wave of the pandemic in the Russian Federation.
- Published
- 2011
25. [Prevalence of high- and low-risk oncogenic human papillomaviruses in patients with external genital pathology].
- Author
-
Vergeichik GI, Stribuk ZhA, and Eremin VF
- Subjects
- Adolescent, Adult, Aged, Aged, 80 and over, Condylomata Acuminata virology, Female, Humans, Middle Aged, Precancerous Conditions virology, Prevalence, Tumor Virus Infections virology, Vaginal Neoplasms virology, Vulvar Neoplasms virology, Condylomata Acuminata pathology, Papilloma pathology, Papillomaviridae pathogenicity, Precancerous Conditions pathology, Tumor Virus Infections pathology, Vaginal Neoplasms pathology, Vulvar Neoplasms pathology
- Abstract
The paper presents the results of examining 49 patients with genital papillomas, vulvar and vaginal leukoplakia, dysplasia, and cancer. The findings may suggest that human papillomavirus plays an important role in the development of vulvar and vaginal lesions and reconsider the importance of high- and low-risk oncogenic genotypes in the development of benign neoplasms, precancerous conditions, and malignant tumors of the vulva and vagina.
- Published
- 2011
26. [Differentiation of genetic variants of Crimean-Congo hemorrhagic fever virus].
- Author
-
Seregin SV, Seregin SS, Petrov VS, Tiunnikov GI, Iakimenko NV, and Sergeev AN
- Subjects
- DNA Primers chemistry, DNA Restriction Enzymes, Genetic Variation, Genome, Viral, Hemorrhagic Fever, Crimean virology, Humans, Nucleic Acid Amplification Techniques, Phylogeography, RNA, Viral analysis, Reverse Transcriptase Polymerase Chain Reaction, Russia, Hemorrhagic Fever Virus, Crimean-Congo genetics, Hemorrhagic Fever, Crimean diagnosis, RNA, Viral genetics, Restriction Mapping
- Abstract
The paper describes a simple, rapid screening of samples potentially containing Crimean-Congo hemorrhagic fever (CCHF) virus strains, by applying the restriction analysis of amplicones, for the differentiation of CCHF virus genotypes that are characteristic of Europe from virus biovariants uncharacteristic of this area, this technique requiring no sequence at the first stage. For this screening, the authors propose to use the PCR fragment of CCHF L segment that comprises a variable region, as well as Alul and Haelll restriction endonucleases. The screening scheme proposed for samples potentially containing CCHF virus may aid investigators to monitor in order to detect uncharacteristic genotypic virus variants in the Russian Federation and other European countries.
- Published
- 2011
27. [Information of the Center for Ecology and Epidemiology of Influenza, D. I. Ivanovsky Research Institute of Virology, Russian Academy of Medical Sciences, on the results of the 2009-2010 influenza and acute respiratory viral infection epidemic season (at week 40 of 2009 to week 22 of 2010) in the world and Russia].
- Author
-
L'vov DK, Burtseva EI, and Lavrishcheva VV
- Subjects
- Antibodies, Viral immunology, Antigens, Viral immunology, Antiviral Agents pharmacology, Antiviral Agents therapeutic use, Drug Resistance, Viral drug effects, Drug Resistance, Viral immunology, Epidemics, Humans, Influenza A virus drug effects, Influenza B virus drug effects, Influenza Vaccines administration & dosage, Influenza Vaccines therapeutic use, Influenza, Human epidemiology, Influenza, Human immunology, Influenza, Human prevention & control, Influenza, Human therapy, Oseltamivir pharmacology, Oseltamivir therapeutic use, Rimantadine pharmacology, Rimantadine therapeutic use, Russia epidemiology, Vaccination, Zanamivir pharmacology, Zanamivir therapeutic use, Influenza A virus pathogenicity, Influenza B virus pathogenicity, Influenza, Human virology
- Abstract
The paper describes the specific features of the 2009-2010 epidemic season in Russia and the world, which are due to the wide spread of a new pandemic strain of influenza A(H1N1)v virus. There is an unusual early upsurge in the incidence of influenza and acute respiratory viral infection (ARVI) (in October-November 2009) with its peak at weeks 45 to 48 of the year with a succeeding reduction to the seasonal values by its end. The circulation of influenza B virus strains was recorded in February-April 2010, which was responsible for the higher epidemic thresholds of morbidity in a number of Russia's regions. A study of the antigenic properties of the strains defined their relationship to the reference strains A/California/07/2009 (H1N1)v and B/Brisbene/60/2008. There were strains with amino acid substitutions at position 222 of hemagglutinin in the population of pandemic influenza A(H1N1)v virus. The strains of the new pandemic influenza A(H1N1)v virus were resistant to remantadine and susceptible to oseltamivir, zanamivir, and arbidol. The influenza B virus strains were susceptible to oseltamivir, zanamivir, and arbidol. The proportion of pathogens of some ARVIs was as follows: parainfluenza viruses, 9.8%; adenoviruses, 5.5%; respiratory syncytial virus, 4.8%; and Mycoplasma pneumonia, 0.6%. There is evidence that there is a need for further monitoring of influenza viruses in Russia.
- Published
- 2011
28. [Sensitivity of the epidemic and pandemic influenza virus strains to zanamivir (Relenze) in in vitro experiments].
- Author
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L'vov DK, Burtseva EI, Galegov GA, Beliakova NV, Shevchenko ES, Kolobukhina LV, Merkulova LN, Prilipov AG, Leneva IA, Baranov NI, Gorelikov VN, and Abramov DD
- Subjects
- Animals, Cell Line, Dogs, Humans, Influenza A virus enzymology, Influenza B virus enzymology, Influenza, Human drug therapy, Influenza, Human virology, Microbial Sensitivity Tests, Neuraminidase antagonists & inhibitors, Oseltamivir pharmacology, Russia epidemiology, Viral Proteins antagonists & inhibitors, Antiviral Agents pharmacology, Disease Outbreaks, Drug Resistance, Viral, Influenza A virus drug effects, Influenza B virus drug effects, Influenza, Human epidemiology, Zanamivir pharmacology
- Abstract
The paper presents the results of the first Russian experience in evaluating the sensitivity of the epidemic and pandemic influenza virus strains, circulating in the period 2009-2010, to the anti-neuraminidase drug zanamivir. A complex of studies, including enzyme immunoassay, fluorometric assay and partial sequence of the neuraminidases (NA1 and NA2) from influenza A virus strain, was applied. The findings Indicate that all the test strains, including those resistant to oseltamivir, were susceptible to zanamivir. The latter is recommended by the WHO for the prevention and treatment of influenza in pregnant women.
- Published
- 2010
29. [Horizontal transmission routes of baculovirus infection in gypsy moth (Lymantria dispar L.)].
- Author
-
Kolosov AV, Kosogova TA, Bulychev LE, and Sergeev AN
- Subjects
- Animals, Larva virology, Baculoviridae, Insect Control methods, Moths virology
- Abstract
The paper considers horizontal transmission routes of baculovirus infection in the gypsy moth (Lymantria dispar L.). The original method for modeling natural processes in controllable conditions allowed one to estimate the influence of factors on the occurrence of epizooties. The authors investigated 3 possible models of virus transmission from infected to uninfected gypsy moths: 1) infected and test caterpillars were kept and fed together (a complex route); 2) those which were in the immediate vicinity, but deprived of eating together (an aerial route); 3) test caterpillars were fed on the leaves on which infected caterpillars had eaten (an oral route). The investigations have shown that the complex and oral routes out of the considered models may be considered to be effective infection transmission routes for the horizontal spread of epizooties. Furthermore, the availability of sufficient amount of infected caterpillars in the population leads to a reduction in the resistance of healthy insects to other diseases. Thus, by taking into account the capacity of larvae for passive migration, the purpose of insecticidal treatment is to set up a few infection foci that will be a source for the spread of epizootias and contribute to an overall viability reduction of a pest population.
- Published
- 2010
30. [Effect of the antiviral drug Ingaviruin on intracellular transformations and import into the nucleus of influenza A virus nucleocapsid protein].
- Author
-
Semenova NP, Prokudina EN, Livov DK, and Nebol'sin VE
- Subjects
- Animals, Caproates, Cell Line, Cell Nucleus metabolism, Cytoplasm metabolism, Dogs, Influenza A Virus, H3N8 Subtype chemistry, Influenza A Virus, H3N8 Subtype drug effects, Nucleocapsid Proteins drug effects, Protein Transport drug effects, Virus Assembly drug effects, Amides pharmacology, Antiviral Agents pharmacology, Dicarboxylic Acids pharmacology, Imidazoles pharmacology, Influenza A Virus, H3N8 Subtype physiology, Nucleocapsid Proteins metabolism, Orthomyxoviridae Infections virology
- Abstract
The paper presents the results of studying the effect of the antiviral drug Ingavirin on different stages of intracellular transformations of influenza A virus nucleocapsid protein (NP). Ingavirin 400-1000 microg/ml has been found to impair the biogenesis of influenza virus NP, to lower the efficiency of formation of conformationally mature compact NP oligomers, and to retard the migration of newly-synthesized NP from the cytoplasm to the nucleus. It is shown that there is an association of tritium-labeled Ingavirin with the nuclear membranes of MDCK cells. The investigations of the mechanisms of antiviral activity of Ingavirin are not only important for the characterization of this drug, but also promote the detection of potential targets to design novel antiviral agents.
- Published
- 2010
31. [Investigation of genetic polymorphism of the integrase gene in the HIV-1 subtype A populations circulating in the Russian Federation].
- Author
-
Gafarova IE, Turbina GI, and Garaev MM
- Subjects
- Codon genetics, Drug Resistance, Viral genetics, Female, HIV Integrase Inhibitors pharmacology, HIV-1 drug effects, Humans, Male, Phylogeny, Russia epidemiology, Genes, pol genetics, HIV Infections epidemiology, HIV Infections virology, HIV Integrase genetics, HIV-1 genetics, Polymorphism, Genetic
- Abstract
The paper presents the data of an investigation of the genetic polymorphism of the pol gene encoding viral integrase (IN) in a HIV subtype A infected population in the Lipetsk Region. The investigators analyzed 32 virus subtype A samples obtained in 2002-2007. Polymorphism at the codons associated with IN resistance to chemicals was observed in 7 virus variants. The found substitutions had a pattern of genetic polymorphism and were unassociated with resistance in 6 patients with the test subtype A population. At the same time, minor RAL resistance mutation was revealed in 1 (3.1%) virus variant while the similar mutations in the subtype G population were about 10%.
- Published
- 2010
32. [A case of Crimean-Congo hemorrhagic fever in the Anapa District, Krasnodar Territory].
- Author
-
Larichev VF, Pilikov OM, Iiunicheva IuV, Gal'tseva GV, Riabova TE, Vasilenko LE, and Butenko AM
- Subjects
- Animals, Antibodies, Viral blood, Bites and Stings virology, Female, Hemorrhagic Fever Virus, Crimean-Congo immunology, Hemorrhagic Fever, Crimean blood, Hemorrhagic Fever, Crimean epidemiology, Humans, Immunoglobulin G blood, Immunoglobulin M blood, Russia epidemiology, Seroepidemiologic Studies, Hemorrhagic Fever Virus, Crimean-Congo isolation & purification, Hemorrhagic Fever, Crimean diagnosis, Ixodes virology
- Abstract
The paper gives information on the first case of Crimean-Congo hemorrhagic fever detected in a female resident of the Anapa District, Krasnodar Territory, in 2005 in the past 57 years.
- Published
- 2010
33. [Etiological characteristics of the influenza epidemics of 2006-2009 in the Russian Federation (according to the data of the Research Institute of Influenza, North-Western Branch, Russian Academy of Medical Sciences)].
- Author
-
Konovalova NI, Eropkin MIu, Gudkova TM, Grigor'eva VA, Danilenko DM, Ivanova AV, Smirnova TS, Lobova TG, and Shchekanova SM
- Subjects
- Animals, Antigens, Viral isolation & purification, Cell Culture Techniques, Chick Embryo, Dogs, Hemagglutination Inhibition Tests, Humans, Influenza A Virus, H1N1 Subtype isolation & purification, Influenza A Virus, H3N2 Subtype isolation & purification, Influenza B virus isolation & purification, Neutralization Tests, Russia epidemiology, Antigens, Viral immunology, Influenza A Virus, H1N1 Subtype immunology, Influenza A Virus, H3N2 Subtype immunology, Influenza B virus immunology, Influenza, Human epidemiology, Influenza, Human virology
- Abstract
The basic trends in the evolution of influenza A and B in the Russian Federation during the epidemic seasons of 2006-2009 were studied on the basis of an antigenic analysis of 1774 Influenza isolated at the Research Institute of Influenza (RII), North-Western Branch, Russian Academy of Medical Sciences, and sent from resting bases (the regional centers of the Russian Inspectorate for the Protection of Consumer Rights and Human Welfare, which collaborate with the RII). Although the trends in the substitution of representative strains generally coincide with the world patterns, the authors revealed some specific features of the antigenic drift of influenza viruses in the Russian Federation and regional varieties. Data on some biological properties and those of the antigenic analysis of the first pandemic influenza A(H1NI)v strains isolated at the RII from Saint Petersburg patients in July-August 2009 are also given in the paper.
- Published
- 2010
34. [A possible association of fatal pneumonia with mutations of pandemic influenza A/H1N1 sw1 virus in the receptor-binding site of the HA1 subunit].
- Author
-
L'vov DK, Burtseva EI, Prilipov AG, Bogdanova VS, Shchelkanov MIu, Bovin NV, Samokhvalov EI, Fediakina IT, Deriabin PG, Kolobukhina LV, Shtyria IuA, Shevchenko ES, Malyshev NA, Merkulova LN, Bazarova MV, Maslov AI, Ishchenko NM, Iskhakova EA, Al'khovskiĭ SV, Grebennikova TV, Sadykova GK, L'vov DN, Zhuravleva MM, Iamnikova SS, Shliapnikova OV, Poglazov AB, Trushakova SV, Lavrishcheva VV, Aristova VA, Proshina ES, Vereshchagin NN, Kuz'michev AG, Iashkulov KB, Dzhambinov SD, Bushkieva BTs, Eliseeva SM, Bystrakov SI, Sokolova IA, Dzhaparidzhe NI, Ledenev IuA, Rosolovskiĭ AP, Gareev RV, Boldyreva VV, Anan'ev VIu, Baranov NI, Gorelikov VN, Garbuz IuA, Reznik VI, Ivanov LI, Zdanovskaia NN, Sergeeva NM, Podolianko IA, Elovskiĭ OV, Gromova MA, Kalaeva EE, Grigor'ev SN, Eremeeva IuV, Dovgal' MV, Fedelesh IIu, Sakharova EA, Burtnik VI, Avdoshina LN, Shapiro NP, Maslov DV, Ianovich VA, Ott VA, and Lebedev GB
- Subjects
- Binding Sites genetics, Female, Hemagglutinin Glycoproteins, Influenza Virus metabolism, Humans, Influenza A Virus, H1N1 Subtype pathogenicity, Influenza, Human mortality, Lung virology, Male, Pneumonia, Viral mortality, Polymerase Chain Reaction, Pregnancy, Pregnancy Complications, Infectious mortality, Protein Subunits metabolism, Receptors, Virus metabolism, Russia epidemiology, Sequence Analysis, Protein, Virulence, Disease Outbreaks, Hemagglutinin Glycoproteins, Influenza Virus genetics, Influenza A Virus, H1N1 Subtype genetics, Influenza, Human epidemiology, Influenza, Human virology, Pneumonia, Viral epidemiology, Pneumonia, Viral virology, Pregnancy Complications, Infectious epidemiology, Pregnancy Complications, Infectious virology, Protein Subunits genetics
- Abstract
The paper gives the results of sequence analysis of 150 positive samples in real-time RT-PCR, including 47 autopsy materials from patients (including 10 pregnant women), who died from fatal pneumonia mainly in November-December 2009, in whom the lifetime etiological diagnosis had not been made and hence no early etiotropic therapy performed. 70% of the primary materials from the deceased patients were found to have pandemic influenza A(H1N1) v mutants in the lung tissue with D222G (15%), D222N (15%), D222E (2%) substitutions, as well as a mixture of mutants (38%). Nasopharyngeal lavages from 3 Chukotka deceased patients exhibited only consensus (nonmutant) D222 virus variants; there was a mixture of consensus and mutant virus variants in the trachea and a mixture of mutant ones in the lung. Preliminary data from the study of the interaction of the hemagglutinin of two strains having D222G and D222N mutations with 9 oligosaccharides imitating the variants of cell receptors for influenza A virus suggest that there is a double receptor specificity for alpha2'-3' and alpha2'-6'-sialosides with a preponderance of alpha2'-3'-specificity. Further spread of the mutants that have acquired a high virulence and preserved their capacity for the respiratory route of human infection may lead to the situation similar to that seen in the 1918-1919 pandemic. Another scenario for evolution of the virus is to preserve its receptor specificity for alpha2'-3'-sialosides and high virulence with losses of alpha2'-6' specificity and capacity for aerosol transmission, by damping the pandemic.
- Published
- 2010
35. [Functional activity of specific antibodies in patients vaccinated against tick-borne encephalitis in relation to different virus strains].
- Author
-
Leonova GN and Pavlenko EV
- Subjects
- Animals, Antibodies, Viral blood, Encephalitis, Tick-Borne blood, Humans, Mice, Time Factors, Antibodies, Viral immunology, Antibody Affinity, Encephalitis Viruses, Tick-Borne immunology, Encephalitis, Tick-Borne immunology, Vaccination, Viral Vaccines immunology
- Abstract
The paper shows variability of the functional activity of the superficial structures of three Far Eastern strains of tick-borne encephalitis (TBE) virus used to study the immunogenicity of different vaccines. The avidity antibodies to the study TBE virus strains were not detected in all of the persons vaccinated with different TBE vaccines even in the year of immunization. Two years after a course of vaccination, immune response and antibody avidity were decreased in the vaccinees. The persons vaccinated with "eastern" vaccines were better protected against Sofjin-like strain P-73 that had led to a fatal outcome in a patient with TBE. Those vaccinated with "western" strain vaccines were found to have a higher affinity for antibodies to the strains P-202 and P-69 that caused inapparent TBE in the year of immunization and 2 years later. A group of persons vaccinated with both "western" and "eastern" vaccines in different periods showed rather high antibody avidities.
- Published
- 2010
36. [Detection of amino acid substitutions of asparaginic acid for glycine and asparagine at the receptor-binding site of hemagglutinin in the variants of pandemic influenza A/H1N1 virus from patients with fatal outcome and moderate form of the disease].
- Author
-
L'vov DK, Iashkulov KB, Prilipov AG, Burtseva EI, Shchelkanov MIu, Shliapnikova OV, Poglazov AB, Sadykova GK, Dzhambinov SD, Fediakina IT, Bushkieva BTs, L'vov DN, Zhuravleva MM, Al'khovskiĭ SV, Samokhvalov EI, Trushakova SV, Lavrishcheva VV, Vereshchagin NN, Mikhaliaeva LB, Darbakova TA, Limanskaia OS, Dzhaparidze NI, Imkenova LN, Ledenev IuA, Boldyreva VV, Ivanov LI, and Zdanovskaia NN
- Subjects
- Adult, Amino Acid Substitution, Asparagine genetics, Binding Sites genetics, Glycine genetics, Hemagglutinin Glycoproteins, Influenza Virus metabolism, Humans, Influenza, Human virology, Middle Aged, Molecular Epidemiology, Receptors, Virus metabolism, Russia epidemiology, Hemagglutinin Glycoproteins, Influenza Virus genetics, Influenza A Virus, H1N1 Subtype genetics, Influenza, Human epidemiology
- Abstract
The paper analyzes the amino acid sequence of the receptor-binding site of hemagglutinin (HA) in the variants of pandemic influenza A/H1N1 swl from 18 patients with moderate (n=1) and fatal (n=17) forms of the disease in 2009. Nine samples contained asparaginic acid at position 222 of HA1 (D). This site exhibited mutations in 9 samples: D222G (n=3), D222N (n=3), and D222G/D222N (n=3). In one patient with the moderate form of the disease, D222G mutation was revealed after the second passage in the developing chick embryos; this mutation was not found in the primary sample from the patient. The findings suggest the mutant variants of the virus start to circulate among the population, which requires, firstly, continuation of molecular virological monitoring of the pandemic situation and, secondly, further study of the impact of amino acid substitutions at the receptor-binding site of HA1 on the increased virulence of influenza A virus.
- Published
- 2010
37. [Trends in the spread of pandemic influenza A(H1N1) swl in the Far East in 2009].
- Author
-
Shchelkanov MIu, L'vov DN, Fediakina IT, Baranov NI, Gorelikov VN, Reznik VIa, Zdanovskaia NI, Pukhovskaia NM, Avdoshina LN, Shapiro NI, Snetkova IP, Kozhan VN, Iarovenko GM, Kalaeva EE, Gromova MA, Elovskiĭ OV, Eremeeva IuV, Dovgal' MA, Kuchenkov AA, Anan'ev VIu, Burtnik VI, Ivanov LI, Garbuz IuA, Podolianko IA, Grigor'ev SN, Proshina ES, Samokhvalov EI, Al'khovskiĭ SV, Burtseva EI, Prilipov AG, Abbasova EI, Mironenko ES, Kolobukhina LV, Deriabin PG, Ott VA, Maslov DV, Ianovich VA, and L'vov DK
- Subjects
- Animals, Antibodies, Viral blood, Cell Line, Chick Embryo, Dogs, Environmental Monitoring, Epidemiological Monitoring, Humans, Influenza A Virus, H1N1 Subtype immunology, Influenza A Virus, H1N1 Subtype isolation & purification, Influenza, Human blood, Influenza, Human diagnosis, Influenza, Human virology, Siberia epidemiology, Disease Outbreaks, Influenza A Virus, H1N1 Subtype classification, Influenza, Human epidemiology
- Abstract
The paper describes the trend in the spread of pandemic influenza A(H1N1) swl virus in the Far East, which started in this region 2-3 months later than that in the European part of Russia. By mid-October seasonal epidemic influenza was practically displaced by pandemic one.
- Published
- 2010
38. [Spread of new pandemic influenza A(H1N1)v virus in Russia].
- Author
-
L'vov DK, Burtseva EI, Shchelkanov MIu, Prilipov AG, Kolobukhina LV, Malyshev NA, Bazarova MV, Merkulova LN, Deriabin PG, Kuz'michev AG, Fediakina IT, Grebennikova TV, Usachev EV, Sadykova GK, Shevchenko ES, Trushakova SV, Lavrishcheva VV, Al'khovskiĭ S, Samokhvalov EI, Beliakova NV, Ivanova VT, Oskerko TA, Latyshev OE, Beliaev AM, Beliaev AL, and Feodoritova EL
- Subjects
- Animals, Antiviral Agents pharmacology, Cell Line, Chick Embryo, Dogs, Genome, Viral genetics, Humans, Influenza, Human mortality, Influenza, Human virology, Russia epidemiology, Sequence Analysis, Protein, Disease Outbreaks, Influenza A Virus, H1N1 Subtype classification, Influenza A Virus, H1N1 Subtype drug effects, Influenza A Virus, H1N1 Subtype genetics, Influenza A Virus, H1N1 Subtype isolation & purification, Influenza, Human epidemiology
- Abstract
The paper presents the results of the investigations of the development of a influenza A(H1N1)v pandemic, conducted by the D. I. Ivanovsky Research Institute of Virology, Russian Academy of Medical Sciences, and collaborating laboratories in the European part of Russia, in the Urals, Siberia, and in the Far East. In the prepandemic period (April 27 - June 11, 2009) its first diagnosis was established on May 21, 2009; the first strain was isolated on May 24, 2009; the data on complete genome sequencing were sent to the GenBank; the sensitivity of the strain to commercial antiviral commercial agents was studied. In the early pandemic period (June 11 - August 15), 73 patients who had come from 14 countries of Europe, America, and Asia were identified; 19 virus strains (partially or completely sequenced) were isolated. The pandemic period (August 15 - December 1) was marked by absolute dominance of pandemic influenza virus virtually in the absence of seasonal influenza; the first death caused by pandemic influenza was detected in late August; 3053 subjects were infected with the pandemic strain, as shown by polymerase chain reaction diagnosis; 202 strains were identified.
- Published
- 2010
39. [Genetic characteristics of the causative agent of tick-borne encephalitis in Mongolia].
- Author
-
Khasnatinov MA, Danchinova GA, Kulakova NV, Tungalag K, Arbatskaia EV, Mironova LV, Tserennorov D, Bolormaa G, Otgonbaatar D, and Zlobin VI
- Subjects
- Adult, Amino Acid Sequence, Amino Acid Substitution, Brain virology, Encephalitis Viruses, Tick-Borne genetics, Encephalitis Viruses, Tick-Borne isolation & purification, Fatal Outcome, Humans, Male, Molecular Sequence Data, Mongolia, Phylogeny, Sequence Alignment, Viral Envelope Proteins genetics, Encephalitis Viruses, Tick-Borne classification, Encephalitis, Tick-Borne virology
- Abstract
A patient with diagnosed meningoencephalitis and a history of tick bite died in Mongolia in 2008. The purpose of this paper is to characterize the virus causing the ill person's death. The virus was identified using the phylogenetic analysis of the 520-bp fragment of the tick-borne encephalitis virus (TBEV) genome, which codes the fragment of TBEV protein E between 52-223 amino acids. TBEV RNA was detected in the samples of medulla oblongata, cerebral cortex, and pia mater of brain, but not in the cerebellar tissue. The study virus fragment was genetically closest to the representatives of the Far East subtype. Its closest relative was virus 740-84 (GenBank EU878282) isolated from large-toothed redback voles (Clethrionomys glareolus) in Buryatia and greatly differed from the Far East virus Soffin. Two amino acid substitutions (H86R and VI7A) were detected within the study protein E fragment. The paper is the first to describe the causative agent of tick-borne encephalitis on the territory of Mongolia and to discuss the evolution and pathogenicity of TBEV.
- Published
- 2010
40. [Chicken yolk IgY preparations as immune reagents to tick-borne encephalitis virus].
- Author
-
Ivanov AP and Vargin VV
- Subjects
- Animals, Antibody Specificity, Antigens, Viral analysis, Antigens, Viral immunology, Encephalitis Viruses, Tick-Borne isolation & purification, Encephalitis, Tick-Borne diagnosis, Enzyme-Linked Immunosorbent Assay, Female, Immunoglobulins isolation & purification, Chickens immunology, Egg Yolk immunology, Encephalitis Viruses, Tick-Borne immunology, Immunoglobulins immunology
- Abstract
This paper provides the first (as is known from the literature analysis) results of the use of specific egg yolk IgY from immunized hens to prepare immune reagents to tick-borne encephalitis virus (TBEV). The IgY preparations are shown to possess a high specific activity in ELISA and can be used as reference reagents, and also for the construction of ELISA systems to detect TBEV antigen.
- Published
- 2010
41. [Dynamics of hantavirus detection in the excretory organs of Apodemus mice and its relation to epidemic manifestations of hantavirus infection].
- Author
-
Slonova RA, Kushnareva TV, Iunikhina OV, Kompanets GG, Maksema IG, and Kushnarev EL
- Subjects
- Animals, Hemorrhagic Fever with Renal Syndrome virology, Intestines virology, Population Density, Salivary Glands virology, Seasons, Siberia epidemiology, Urinary Bladder virology, Disease Reservoirs virology, Orthohantavirus isolation & purification, Hemorrhagic Fever with Renal Syndrome epidemiology, Hemorrhagic Fever with Renal Syndrome veterinary, Murinae virology
- Abstract
This paper presents data on the detection of hantavirus in the excretory organs of Apodemus mice which are natural carriers of pathogenic hantaviral serotypes in the Primorye Region. Acute infection periods were detected in naturally infected rodents with the highest hantavirus dissemination to the environment in different phases of rodent population cycles. There was a relationship between the rise of morbidity and the seasonal manifestation of hemorrhagic fever with renal syndrome morbidity and activity of hantavirus reproduction in the murine excretory organs.
- Published
- 2010
42. [S3 gene fragment sequence analysis in the chicken reovirus isolates detected in the poultry farms of the Russian Federation].
- Author
-
Ziniakov NG, Andreĭchuk DB, and Drygin VV
- Subjects
- Animals, Base Sequence, Capsid Proteins classification, Molecular Sequence Data, Phylogeny, RNA-Binding Proteins classification, Reoviridae isolation & purification, Reoviridae Infections virology, Russia, Sequence Analysis, DNA, Sequence Homology, Nucleic Acid, Capsid Proteins genetics, Chickens genetics, Poultry Diseases virology, RNA-Binding Proteins genetics, Reoviridae genetics, Reoviridae Infections veterinary
- Abstract
The paper presents data on the comparative analysis of nucleotide sequences of a S3 gene fragment of 67 chicken reovirus (CRV) isolates from the abnormal biopsy specimens tested in 1999 to 2007. These CRV isolates were ascertained to differ from vaccine strains in the nucleotide sequence of the S3 gene. The approximate substitution rates for the S3 gene were established to range from 2.0 x 10(-3) to 6.0 x 10(-3) nucleotide substitutions per year.
- Published
- 2010
43. [PHOSPHAN microplate technology-based microarray for the determination of IgG antibodies against West Nile and Crimean-Congo hemorrhagic fever viruses].
- Author
-
Pomelova VG, Bychenkova TA, Laricheva SIu, Osin NS, Butenko AM, Larichev VF, and Khutoretskaia NV
- Subjects
- Antibodies, Viral immunology, Antibody Specificity, Hemorrhagic Fever Virus, Crimean-Congo immunology, Hemorrhagic Fever, Crimean blood, Humans, Immunoglobulin G immunology, Sensitivity and Specificity, West Nile Fever blood, West Nile virus immunology, Antibodies, Viral blood, Fluorescent Antibody Technique, Hemorrhagic Fever Virus, Crimean-Congo isolation & purification, Hemorrhagic Fever, Crimean diagnosis, Immunoglobulin G blood, Protein Array Analysis methods, West Nile Fever diagnosis, West Nile virus isolation & purification
- Abstract
The paper demonstrates it possible to work out a phosphorescence analysis (PHOSPHAN) microplate technology-based microarray for concurrently examining human sera and detection of their specific IgG antibodies against two heterological West Nile and Crimean-Congo hemorrhagic fever viruses. The sensitivity and specificity of the microarray were comparable with those of enzyme immunoassay with separate sample testing. The advantages of PHOSPHAN were associated with the microplate format of an immunoassay and its enhanced multiplexity, which may contribute to the lower cost of clinical sample testing.
- Published
- 2010
44. [Molecular epidemiology of parvovirus infection in Belarus].
- Author
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Ermoloich MA, Semeĭko GV, Samoĭlovich EO, Hubschen JM, and Muller CP
- Subjects
- Adolescent, Adult, Child, Child, Preschool, Humans, Molecular Epidemiology, Parvoviridae Infections virology, Parvovirus B19, Human classification, Parvovirus B19, Human isolation & purification, Phylogeny, Republic of Belarus epidemiology, Viral Nonstructural Proteins genetics, Viral Structural Proteins genetics, Parvoviridae Infections epidemiology, Parvovirus B19, Human genetics
- Abstract
The paper analyzes a 994 nucleotide fragment of the NS1/VP1u region junction of 84 parvovirus B19 samples obtained from the sera of erythema infectiosum patients in Belarus in 2006. All the strains belong to genotype 1 as defined by Servant et al. (2002) and form two major clusters within this genotype (1A and 1B) with a clearly distinct geographic distribution. Cluster 1A mainly included B19 strains from Minsk where an outbreak of erythema infectiosum was observed during sample collection. Cluster 1B comprises parvovirus B19 strains obtained from sporadic cases in different parts of the country. The nucleotide variability within cluster 1B (1.1%) was almost two times higher than that within cluster 1A (0.6%). The comparison of the Belarus strains with all parvovirus B19 sequences from the GenBank revealed 22 unique nucleotide substitutions in the new strains, 18 (81.8%) of which were nonsynonymous. A high percentage of parvovirus B19 IgM positive sera were also PCR positive (94.0%; n = 63/67) indicating that both methods are suitable for diagnosis of the infection.
- Published
- 2010
45. [Analysis of HIV-1 integrase gene polymorphism in an HIV-infected population from the nosocomial outbreak of HIV infection in the south of Russia in 1989].
- Author
-
Gafarova IE, Shideeva ZhA, Sandzhieva DB, and Garaev MM
- Subjects
- Cross Infection epidemiology, Female, HIV Infections virology, HIV Integrase drug effects, HIV Integrase Inhibitors pharmacology, HIV-1 drug effects, Humans, Male, Molecular Epidemiology, Polymorphism, Genetic, Russia epidemiology, Cross Infection virology, Disease Outbreaks, Drug Resistance, Viral genetics, Genes, pol, HIV Infections epidemiology, HIV Integrase genetics, HIV-1 genetics
- Abstract
The paper presents the data of an investigation of the polymorphism of the pol gene encoding HIV-1 integrase in a HIV subtype G infected population formed during the 1989 HIV-infection outbreak. The investigators analyzed 41 samples of the viruses obtained in 2005-2007. Polymorphism at codons associated with integrase resistance to chemicals was observed in 11 virus variants. The circulation of mutation viruses that potentially promote the formation of resistance to the integrase inhibitors raltegravir and elvitegravir has been established in untreated patients.
- Published
- 2010
46. [Localization of human cytomegalovirus immediate-early protein IE-1 pp72 in the juxtanuclear inclusion at the late stage of infection].
- Author
-
Naumenko VA, Manykin AA, Gushchina EA, Fedorova NE, Pavlova MV, and Kushch AA
- Subjects
- Cell Nucleus metabolism, Cell Nucleus virology, Cells, Cultured, Cytomegalovirus Infections metabolism, Cytoplasm metabolism, Fibroblasts metabolism, Fibroblasts virology, Fluorescent Antibody Technique, Humans, Immediate-Early Proteins genetics, Inclusion Bodies, Viral ultrastructure, Microscopy, Electron, Virus Replication, Cytomegalovirus metabolism, Cytomegalovirus Infections virology, Cytoplasm virology, Immediate-Early Proteins metabolism, Inclusion Bodies, Viral metabolism
- Abstract
Immediate-early protein IE-1 pp72 is one of the most abundant proteins at the early stage of human cytomegalovirus infection and has a number of intranuclear activities. This paper gives immunocytochemical and ultrastructural data on IE-1 pp72 accumulation in the juxtanuclear inclusion at the late stage of low-multiplicity infection. Detection of a new localization site infers that this protein may participate in the final steps of virus morphogenesis and play a functional role in the pathogenesis of cytomegalovirus infection.
- Published
- 2009
47. [The 24 May, 2009 isolation of the first A/IIV-Moscow/01/2009 (H1N1)swl strain similar to swine A(H1N1) influenza virus from the first Moscow case detected on May 21, 2009, and its deposit in the state collection of viruses (SCV No. 2452 dated May 24, 2009)].
- Author
-
L'vov DK, Burtseva EI, Prilipov AG, Bazarova MV, Kolobukhina LV, Merkulova LN, Malyshev NA, Deriabin PG, Fediakina IT, Sadykova GK, Usachev EV, Shchelkanov MIu, Shevchenko ES, Trushakova SV, Ivanova VT, Beliakova NV, Oskerko TA, and Aliper TI
- Subjects
- Amino Acid Substitution, Animals, Antiviral Agents pharmacology, Cell Line, Chick Embryo, Drug Resistance, Viral, Genome, Viral, Humans, Indoles pharmacology, Influenza A Virus, H1N1 Subtype drug effects, Influenza A Virus, H1N1 Subtype genetics, Microbial Sensitivity Tests, Molecular Sequence Data, Moscow epidemiology, Neuraminidase genetics, Oseltamivir pharmacology, Ribavirin pharmacology, Rimantadine pharmacology, Travel, Viral Proteins genetics, Influenza A Virus, H1N1 Subtype isolation & purification, Influenza, Human epidemiology, Influenza, Human virology
- Abstract
The paper presents the results of the first isolation of the new influenza virus in Moscow and the Russian Federation, which was similar to the swine A/IIV-Moscow/01/2009(H1N1)swl strain isolated on May 24, 2009 from a Russian arrived in Moscow from the USA on May 19, 2009. The antigenic, biological, and molecular genetic properties of this virus were studied. The virus was isolated on MDCK and chick embryos, the hemagglutination titers being 1:8-1:16 AE; the infectious titers being 6.51g of the tissue cytopathogenic infective dose (TCID50) and 7.01g of the common infective dose (CID50). The virus was sensitive to arbidol, ribavirin, oseltamivir, and resistant to rimantadine. The complete virus genome was sequenced; the data were accepted to the Gen Bank on May 28, 2009 under GQ219584-GQ219590 and GQ202724. The significant gene substitution of neuraminidase Asp for Gly in position 451, which has been undetectable in any other strain published in the Gen Bank by the present time is unique only to A/IIV-Moscow/01/2009 (H1N1)swl. The virus has been deposited in the State Collection of Viruses, D. I. Ivanovsky Institute of Virology, Russian Academy of Medical Sciences, under No. 2452 dated May 24, 2009.
- Published
- 2009
48. [Monitoring of the sensitivity of epidemic influenza virus strains isolated in Russia to etiotropic chemical agents].
- Author
-
Burtseva EI, Shevchenko ES, Beliakova NV, Oskerko TA, Kolobukhina LV, Merkulova LN, Vartanian RV, Prilipov AG, Rotanov M, and Zaplatnikov AL
- Subjects
- Disease Outbreaks, Drug Resistance, Viral genetics, Epidemiological Monitoring, Humans, Indoles pharmacology, Influenza A Virus, H1N1 Subtype genetics, Influenza A Virus, H1N1 Subtype isolation & purification, Influenza A Virus, H3N2 Subtype genetics, Influenza A Virus, H3N2 Subtype isolation & purification, Microbial Sensitivity Tests, Mutation, Oseltamivir pharmacology, Rimantadine pharmacology, Russia epidemiology, Antiviral Agents pharmacology, Environmental Monitoring, Influenza A Virus, H1N1 Subtype drug effects, Influenza A Virus, H3N2 Subtype drug effects, Influenza, Human epidemiology, Influenza, Human virology
- Abstract
The paper presents the results of studying the spectrum of influenza A and B viruses to rimantadine, arbidol, and oseltamivir and describes the methods used for these purposes for epidemiological surveillance. Different sensitivities to rimantadine were found among influenza A viruses. During the 2007-2008 season, the vast majority of influenza A(H3N2) virus strains were resistant to rimantadine (77%) while all influenza A(H1N1) virus strains preserved their resistance to this drug. The fact that the epidemic influenza A(H1N1) virus strains that carry the mutation responsible for resistance to the neuraminidase inhibitor oseltamivir (Tamiflu) circulated in the Russian Federation was first established. At the same time all the study influenza A(H1N1) virus strains preserved their susceptibility to rimantadine. The sensitivity of the epidemic strains to arbidol has been confirmed.
- Published
- 2009
49. [Isolation of noncytopathogenic genotype 2 bovine viral diarrhea virus from the cattle mucosa in the Russian Federation].
- Author
-
Glotov AG, Glotova TI, Iuzhakov AG, Zaberezhnyĭ AD, and Aliper TI
- Subjects
- 5' Untranslated Regions genetics, Animals, Cattle, Diarrhea Viruses, Bovine Viral genetics, Diarrhea Viruses, Bovine Viral isolation & purification, Nucleocapsid Proteins genetics, Phylogeny, Russia, Viral Envelope Proteins genetics, Bovine Virus Diarrhea-Mucosal Disease virology, Diarrhea Viruses, Bovine Viral classification
- Abstract
The paper presents the results of genotyping and phylogenetic analysis of three noncytopathogenic isolates of bovine viral diarrhea virus from the mucosae of the cattle with different clinical presentations of the disease. On the basis of the phylogenetic analysis of high-conserved and variable virus genome regions (5'-UTR, N(pro), and E2), the authors referred two isolates to as genotype 1, subgenotypes 1b and 1d, and the third isolate to as genotype 2. This is the first communication about the isolation of genotype 2 virus in Russia.
- Published
- 2009
50. [Use of atomic force microscopy to study the cytomorphological signs of papillomavirus infection].
- Author
-
Iakovleva VA, Lisitsyn FV, Manykin AA, and Konysheva TA
- Subjects
- Adult, Epithelial Cells ultrastructure, Female, Genitalia, Female ultrastructure, Humans, Microscopy, Electron, Scanning, Papillomavirus Infections complications, Vulvovaginitis virology, Epithelial Cells pathology, Genitalia, Female pathology, Microscopy, Atomic Force methods, Papillomaviridae, Papillomavirus Infections pathology, Vulvovaginitis pathology
- Abstract
The paper presents the results of studying genital squamous epitheliocytes from human papillomavirus-infected female patients by cytology and atomic force microscopy. The squamous epitheliocytes with and without cytomorphological signs of papillomavirus infection (koilocytosis, dyskeratosis, parakeratosis, hyperkeratosis) have been compared. Examining the surface of the squamous epithelium has yielded quantitative characteristics of infection-induced surface changes.
- Published
- 2009
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