1. Re-Emergence of DENV-3 in French Guiana: Retrospective Analysis of Cases That Circulated in the French Territories of the Americas from the 2000s to the 2023-2024 Outbreak.
- Author
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Lagrave A, Enfissi A, Tirera S, Demar MP, Jaonasoa J, Carod JF, Ramavoson T, Succo T, Carvalho L, Devos S, Dorleans F, Leon L, Berlioz-Arthaud A, Musso D, Lavergne A, and Rousset D
- Subjects
- French Guiana epidemiology, Humans, Retrospective Studies, Guadeloupe epidemiology, Martinique epidemiology, High-Throughput Nucleotide Sequencing, RNA, Viral genetics, Phylogeny, Disease Outbreaks, Dengue Virus genetics, Dengue Virus classification, Dengue Virus isolation & purification, Dengue epidemiology, Dengue virology, Genotype
- Abstract
French Guiana experienced an unprecedented dengue epidemic during 2023-2024. Prior to the 2023-2024 outbreak in French Guiana, DENV-3 had not circulated in an epidemic manner since 2005. We therefore studied retrospectively the strains circulating in the French Territories of the Americas (FTA)-French Guiana, Guadeloupe, and Martinique-from the 2000s to the current epidemic. To this end, DENV-3 samples from the collection of the National Reference Center for Arboviruses in French Guiana (NRCA-FG) were selected and sequenced using next-generation sequencing (NGS) based on Oxford Nanopore Technologies, ONT. Phylogenetic analysis showed that (i) the 97 FTA sequences obtained all belonged to genotype III (GIII); (ii) between the 2000s and 2013, the regional circulation of the GIII American-I lineage was the source of the FTA cases through local extinctions and re-introductions; (iii) multiple introductions of lineages of Asian origin appear to be the source of the 2019-2021 epidemic in Martinique and the 2023-2024 epidemic in French Guiana. Genomic surveillance is a key factor in identifying circulating DENV genotypes, monitoring strain evolution, and identifying import events.
- Published
- 2024
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