1. Mutations in the 3A genomic region of two cytopathic strains of hepatitis A virus isolated in Italy
- Author
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Graziella Morace, Maurizio Divizia, Giulio Pisani, Augusto Panà, and Francesca Beneduce
- Subjects
Cancer Research ,Genes, Viral ,Settore MED/42 - Igiene Generale e Applicata ,Molecular Sequence Data ,Biology ,medicine.disease_cause ,Virus ,Cytopathogenic Effect, Viral ,Virology ,Aspartic acid ,medicine ,Humans ,Nucleotide ,Viral ,Hepatovirus ,Gene ,Cytopathic effect ,chemistry.chemical_classification ,Aspartic Acid ,Mutation ,Base Sequence ,Strain (chemistry) ,Italy ,Gene Deletion ,Phenotype ,Molecular biology ,Infectious Diseases ,Genes ,chemistry ,Cytopathogenic Effect - Abstract
Two strains of hepatitis A virus (HAV) were isolated in cell culture and found to induce a cytopathic effect at early passages. The nucleotide sequences of the 5' non-translated region (5'NTR) and of genes 2B, 2C, 3A and 3B were determined for these strains and found to contain mutations similar to those detected in cell-culture adapted variants of HAV strain HM175. In addition, gene 3A shows a deletion of three aspartic acid residues near the N-terminus of the polypeptide. In combination with variations in the 5'NTR and in genes 2B and 2C, the absence of an aspartic acid residue in position 4 of gene 3A of three cytopathic clones of HM175 suggests a possible role of the 3A protein in determining the cytopathic phenotype.
- Published
- 1993
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