1. Combination of ELISA screening and seroneutralisation tests to expedite Zika virus seroprevalence studies
- Author
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Elif Nurtop, Paola Mariela Saba Villarroel, Boris Pastorino, Laetitia Ninove, Jan Felix Drexler, Yelin Roca, Bouba Gake, Audrey Dubot-Peres, Gilda Grard, Christophe Peyrefitte, Stéphane Priet, Xavier de Lamballerie, and Pierre Gallian
- Subjects
Zika virus ,Seroepidemiology ,Virus neutralization test ,Infectious and parasitic diseases ,RC109-216 - Abstract
Abstract Here we propose a strategy allowing implementing efficient and practicable large-scale seroepidemiological studies for Zika Virus (ZIKV). It combines screening by a commercial NS1 protein-based Zika IgG ELISA, and confirmation by a cytopathic effect-based virus neutralization test (CPE-based VNT). In post-epidemic samples from Martinique Island blood donors (a population with a dengue seroprevalence above 90%), this strategy allowed reaching specificity and sensitivity values over 98%. The CPE-based VNT consists of recording CPE directly under the optical microscope, which is easy to identify with ZIKV strain H/PF/2013 at day 5 pi. Overall, considered that CPE-based VNT is cost effective and widely automatable, the NS1 protein-based Zika IgG ELISA+CPE-based VNT combination strategy represents a convenient tool to expedite ZIKV seroprevalence studies.
- Published
- 2018
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