1. Defining the human immunodeficiency virus type 1 transmission genetic bottleneck in a region with multiple circulating subtypes and recombinant forms
- Author
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Gama Bandawe, Andile Nofemela, Leonard Maboko, Michael Hoelscher, Zenda Woodman, Ruwayhida Thebus, Natasha T. Wood, Jinny C. Marais, Carolyn Williamson, and Oliver Hoffmann
- Subjects
Molecular Sequence Data ,Hiv epidemic ,Human immunodeficiency virus (HIV) ,HIV Infections ,Genome amplification ,Hiv testing ,Biology ,medicine.disease_cause ,Tanzania ,Bottleneck ,law.invention ,Cohort Studies ,law ,Virology ,medicine ,Humans ,Transmission ,Genetic variability ,Phylogeny ,Recombination, Genetic ,Transmission (medicine) ,HIV ,Population bottleneck ,Mbeya ,HIV-1 ,Recombinant DNA ,Female - Abstract
The Mbeya region of Tanzania has a genetically complex HIV epidemic with multiple subtypes and recombinant forms circulating, together with a high frequency of dual infections with more than one subtype. This study aimed to determine whether this impacted the HIV-1 transmission bottleneck. A total of 210 env sequences from 22 participants were generated from recently infected women from Mbeya using the single genome amplification approach. Participants were infected with subtypes C ( n = 9), A ( n = 4), or D ( n = 1), and recombinants AC ( n = 4), CD ( n = 2), AD ( n = 1), or ACD ( n = 1). Sixteen participants (73%) were infected with a single variant; five (23%) with multiple variants; and one (4%) was dually infected. Thus the frequency of single variant infections was similar to cohorts located in genetically restricted subtype B or C epidemics, suggesting that multiple circulating subtypes and unique recombinant forms do not have a significant impact on the transmission bottleneck.
- Published
- 2011