1. Characterizing monkeypox virus specific CD8+ T cell epitopes in rhesus macaques
- Author
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Alessandro Sette, Joseph E. Blaney, Nicole Josleyn, John Sidney, Roger W. Wiseman, Melanie Cohen, Haifeng Song, and Peter B. Jahrling
- Subjects
Enzyme-Linked Immunospot Assay ,Viral protein ,animal diseases ,viruses ,T cell ,Cowpox ,Epitopes, T-Lymphocyte ,CD8-Positive T-Lymphocytes ,Major histocompatibility complex ,medicine.disease_cause ,Article ,Epitope ,Interferon-gamma ,chemistry.chemical_compound ,Virology ,medicine ,Animals ,Cytotoxic T cell ,Monkeypox virus ,biology ,Histocompatibility Antigens Class I ,virus diseases ,biology.organism_classification ,medicine.disease ,Macaca mulatta ,Molecular biology ,medicine.anatomical_structure ,chemistry ,biology.protein ,Vaccinia ,Protein Binding - Abstract
To characterize T cell epitopes in monkeypox virus (MPXV) infected rhesus macaques, we utilized IFN γ Elispot assay to screen 400 predicted peptides from 20 MPXV proteins. Two peptides from the F8L protein, an analog of E9L protein in vaccinia, were found to elicit CD8+ T cell responses. Prediction and in vitro MHC binding analyses suggest that one is restricted by Mamu-A1 ⁎ 001 and another by Mamu-A1 ⁎ 002. The Mamu-A1 ⁎ 002 epitope is completely identical in all reported sequences for variola, vaccinia, cowpox and MPXV. The Mamu-A1 ⁎ 001 epitope is conserved in MPXV and vaccinia, and has one residue substitution (V6>I) in some cowpox sequences and all variola sequences. Given CD8+ T-cell epitopes from E9L were also identified in humans and mice, our data suggested that F8L/E9L may be a dominant pox viral protein for CD8+ T cell responses, and may be considered as a target when designing vaccines that target pox-specific T cell responses.
- Published
- 2013
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