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Your search keyword '"Kanda, Tadahito"' showing total 21 results
Start Over Author "Kanda, Tadahito" Journal virology
21 results on '"Kanda, Tadahito"'

13. Independent association of antibodies against human papillomavirus type 16 and E7 proteins with cervical cancer

Author

Kanda, Tadahito, primary, Onda, Takashi, additional, Zanma, Soichi, additional, Yasugi, Toshiharu, additional, Furuno, Akemi, additional, Watanabe, Sumie, additional, Kawana, Takashi, additional, Sugase, Motoyasu, additional, Ueda, Kuniaki, additional, Sonoda, Takahiko, additional, Suzuki, Saburo, additional, Yamashiro, Takenobu, additional, Yoshikawa, Hiroyuki, additional, and Yoshiike, Kunito, additional

Published
1992
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20. CCAAT/enhancer binding protein β binds to and activates the P670 promoter of human papillomavirus type 16

Author

Kukimoto, Iwao, Takeuchi, Takamasa, and Kanda, Tadahito

Subjects
*KERATINOCYTES, *CELLS, *HEREDITY, *BIOCHEMISTRY, *GENES
Abstract

Abstract: The P670 promoter of HPV16 directs transcription of the virus late genes in the differentiating epithelium. We found that CCAAT/enhancer binding protein β (C/EBPβ), a key transcription factor that induces the terminal differentiation of keratinocytes, enhanced the P670-driven transcription in transient reporter assays in HeLa cells and human primary keratinocytes, whereas it inhibited, as reported previously, the transcription from the early P97 promoter. An electrophoretic mobility shift analysis identified two binding sites in the upstream region of P670 for a bacterially expressed C/EBPβ. A chromatin immunoprecipitation analysis demonstrated that C/EBPβ bound to these sites of the P670 reporter plasmid in HeLa cells. Nucleotide substitutions in these sites in the reporter plasmid abrogated the enhancement by C/EBPβ in the transient HeLa and keratinocyte assays, indicating that the C/EBPβ-binding to these sites is required for the enhancement of transcription from P670. These results suggest that C/EBPβ is involved in enhancing transcription from the P670 during keratinocyte differentiation. [Copyright &y& Elsevier]

Published
2006
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21. CCAAT/enhancer binding protein beta binds to and activates the P670 promoter of human papillomavirus type 16.

Author

Kukimoto I, Takeuchi T, and Kanda T

Subjects
Cell Differentiation, Enhancer Elements, Genetic, HeLa Cells, Human papillomavirus 16 metabolism, Humans, Keratinocytes cytology, Keratinocytes virology, Oncogene Proteins, Viral metabolism, Papillomavirus E7 Proteins, CCAAT-Enhancer-Binding Protein-beta metabolism, Human papillomavirus 16 genetics, Oncogene Proteins, Viral genetics, Promoter Regions, Genetic
Abstract

The P670 promoter of HPV16 directs transcription of the virus late genes in the differentiating epithelium. We found that CCAAT/enhancer binding protein beta (C/EBPbeta), a key transcription factor that induces the terminal differentiation of keratinocytes, enhanced the P670-driven transcription in transient reporter assays in HeLa cells and human primary keratinocytes, whereas it inhibited, as reported previously, the transcription from the early P97 promoter. An electrophoretic mobility shift analysis identified two binding sites in the upstream region of P670 for a bacterially expressed C/EBPbeta. A chromatin immunoprecipitation analysis demonstrated that C/EBPbeta bound to these sites of the P670 reporter plasmid in HeLa cells. Nucleotide substitutions in these sites in the reporter plasmid abrogated the enhancement by C/EBPbeta in the transient HeLa and keratinocyte assays, indicating that the C/EBPbeta-binding to these sites is required for the enhancement of transcription from P670. These results suggest that C/EBPbeta is involved in enhancing transcription from the P670 during keratinocyte differentiation.

Published
2006
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