1. Characterization of a Chemokine Receptor-Related Gene in Human Herpesvirus 8 and Its Expression in Kaposi's Sarcoma
- Author
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Yi Wen Jiang, Mark Raffeld, Sandra Colombini, Gary S. Hayward, Marvin S. Reitz, Hong Guang Guo, Erwin Tschachler, Robert C. Gallo, Mariola Sadowska, John Nicholas, and Philip J. Browning
- Subjects
CCR2 ,Genes, Viral ,viruses ,Molecular Sequence Data ,C-C chemokine receptor type 7 ,C-C chemokine receptor type 6 ,CCR8 ,Biology ,Receptors, Interleukin-8A ,Open Reading Frames ,Viral Proteins ,Chemokine receptor ,Capsid ,Antigens, CD ,hemic and lymphatic diseases ,Virology ,medicine ,Humans ,Amino Acid Sequence ,Receptors, Cytokine ,Sarcoma, Kaposi ,Kaposi's sarcoma ,AIDS-Related Opportunistic Infections ,Base Sequence ,Sequence Homology, Amino Acid ,Interleukin-8 ,virus diseases ,Receptors, Interleukin ,medicine.disease ,DNA, Viral ,Herpesvirus 8, Human ,XCL2 ,Receptors, Chemokine ,CCL21 - Abstract
Human herpesvirus 8 (HHV-8) is a recently discovered virus that is highly associated with Kaposi's sarcoma (KS) and AIDS-associated body cavity lymphomas, although it is also found in some normal individuals. HHV-8 is related by nucleotide sequence homology to herpesvirus saimiri (HVS), which causes T cell lymphomas in some New World monkeys, and to Epstein–Barr virus (EBV), a human herpesvirus linked etiologically with Burkitt's lymphoma and nasopharyngeal carcinoma. We report that, like HVS but unlike EBV, HHV-8 contains a gene (ORF74) with significant sequence homology to the high-affinity IL-8 receptor, a member of the α (CXC) chemokine receptor family of transmembrane G protein-coupled receptors. We also show by reverse transcription PCR that the chemokine receptor-related HHV-8 gene is detectable in some RNA samples from KS tissue, and that its expression varies independently from that of ORF26, a minor capsid protein. The presence of a potential chemokine receptor in HHV-8 and its expression in KS tissue suggests that it may be important in the regulation of viral gene expression and may play a role in the etiology of KS and AIDS-related body cavity lymphomas.
- Published
- 1997