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In this study species specific unique Hypothetical Proteins (HPs from 15 mastitis causing organisms viz. Staphylococcus aureus, E. coli, Enterococcus faecalis, Klebsiella pneumoniae, Pasteurella multocida, Pseudomonas aeruginosa, Klebsiella oxytoca, Staphylococcus epidermidis, Staphylococcus hyicus, Staphylococcus haemolyticus, Staphylococcus chromogenes, Streptococcus agalactiae, Streptococcus dysgalactiae, Streptococcus uberis and Enterococcus aerogenes were identified and characterized. The HPs were selected on the basis of their in vivo stability, species specificity and uniqueness. These HP were characterized in silico for their physico-chemical properties and functions. The results showed well-defined functions of the selected HPs and categorized them either as enzyme, transporter and cellular regulatory proteins. Three HPs showed adhesion properties which can be crucial for the persistence of the organisms and pathogenicity. The outcome of the present study gave better understanding of selected HPs whose functions otherwise had not been predicted. Most of the HPs were related to survival or pathogenesis of the bacteria to which they belonged and being unique to a species of bacteria they can further be used as biomarkers for species identification [ABSTRACT FROM AUTHOR]

The present paper highlights the satisfaction level of respondents those received expert advisory services rendered by RAJUVAS toll free helpline (1800-180-6224) services (RTHS). Total 150 livestock owners were selected from Bikaner and Jaipur district of Rajasthan who contacted RAJUVAS toll free helpline (1800-180-6224) centre (RTHS) for seeking expert advice for their quires. Selected respondents from each district were personally contacted through telephonic interview to collect primary data. Results shown that large number of respondents (78%) were found satisfied with the expert advices as per received from RTHS. However, overall extent of satisfaction level towards advices was 73.33 per cent. Respondents shown their maximum satisfaction (91.33%) towards behaviour of veterinary experts followed by knowledge competency of RTHS experts (90.66%), problem solving competency of RTH experts (89%), accessibility of RTH experts (87.66%), satisfaction for relevancy of expert advice towards farmer’s query (81.33%) and competency of experts in promptness of reply (81%). It was also revealed that respondents were highly satisfied (86.21%) towards overall services of RTHS. Results pertaining to query wise satisfaction level revealed that advices related to medicinal problems had maximum (80.20%) satisfaction level followed by livestock production and management problems (63.33%) and gynaecological problems (61.76%). [ABSTRACT FROM AUTHOR]

In present study, six different protocols for bacterial DNA extraction from milk including classical PCI protocol and one commercial kit, were compared. The assessment was done on the basis of estimated quantity and quality using spectrophotometric measurements and their amplification in PCR. Although, all the methods extracted DNA successfully but Guanidine thiocyanate-Dithioerythritol method and commercial kit were found to be more efficient in terms of time taken for the extraction, purity and concentration of DNA obtained. The DNA extracted was efficiently detected in PCR using both the protocols. [ABSTRACT FROM AUTHOR]

Present paper highlighted the adoption level of livestock owners for the RAJUVAS toll free expert advisory services (1800-1806224). Respondents were selected from two districts Bikaner and Jaipur of Rajasthan state on the basis of received maximum telephonic calls during a period of six months (1 Jan to 30 June, 2017). A total 75 respondents were selected randomly from each district and constitute total 150 respondents as sample for the study. Primary data were generated by contacting the respondents via telephonic interview schedule. The results shown that majority of respondents (56.67%) fully adopted the advices whereas, 33.33 per cent respondents did not, and remaining 10 per cent respondents adopted the advices partially. Results pertaining to query wise adoption level of advices revealed that medicinal problems based adoption level was maximum (65.34%) followed by animal production and management related practices (60%), gynaecological problems (58.82%), animal feeding and nutrition problems (50%), dairy entrepreneurial and dairy marketing related queries (44.44%) and surgical problems (33.33%). [ABSTRACT FROM AUTHOR]