9 results on '"Luján L"'
Search Results
2. Small Ruminant Lentivirus–Induced Arthritis
- Author
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Pérez, M., primary, Biescas, E., additional, Reina, R., additional, Glaria, I., additional, Marín, B., additional, Marquina, A., additional, Salazar, E., additional, Álvarez, N., additional, de Andrés, D., additional, Fantova, E., additional, Badiola, J. J., additional, Amorena, B., additional, and Luján, L., additional
- Published
- 2014
- Full Text
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3. Small Ruminant Lentivirus–Induced Arthritis: Clinicopathologic Findings in Sheep Infected by a Highly Replicative SRLV B2 Genotype.
- Author
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Pérez, M., Biescas, E., Reina, R., Glaria, I., Marín, B., Marquina, A., Salazar, E., Álvarez, N., de Andrés, D., Fantova, E., Badiola, J. J., Amorena, B., and Luján, L.
- Subjects
SHEEP diseases ,ARTHRITIS in animals ,EPIDEMIC research ,LENTIVIRUS diseases ,ANIMAL diseases ,RUMINANTS ,VIRAL replication ,VETERINARY clinical pathology - Abstract
We describe the clinicopathologic features of an arthritis outbreak in sheep induced by small ruminant lentivirus (SRLV), linked to the presence of a new SRLV isolate phylogenetically assigned to caprine arthritis encephalitis virus–like subgroup B2. Thirteen SRLV seropositive Rasa Aragonesa adult ewes were selected from 5 SRLV highly infected flocks (mean seroprevalence, 90.7%) for presenting uni- or bilateral chronic arthritis in the carpal joint. A complete study was performed, including symptomatology, histopathology, immunocytochemistry, immunohistochemistry, in situ hybridization, and microbiology. The carpus was the joint almost exclusively affected, with 10 sheep (76%) showing a moderate increase in carpal joint size (diameter range, 18–20 cm; normal range, 15–16 cm) without signs of locomotion problems and with 3 ewes (23%) showing severe inflammation with marked increase in diameter (21–24 cm), pain at palpation, and abnormal standing position. Grossly, chronic proliferative arthritis was observed in affected joints characterized by an increased thickness of the synovial capsule and synovial membrane proliferation. Microscopically, synovial membrane inflammation and proliferation and hyperplasia of synoviocytes were observed. More positive cases of SLRV infection were detected by immunocytochemistry of articular fluid than of bronchoalveolar lavage fluid. Immunohistochemistry and in situ hybridization also detected positive cells in the subsynovial connective tissue, lung, mediastinal lymph node, mammary gland, and mammary lymph node. All animals were negative for the presence of Mycoplasma or other bacteria in the articular space. The present outbreak likely represents an adaptation of a caprine virus to sheep. Our results underline the importance of the arthritis induced by SRLV in sheep, a clinical form that might be underestimated. [ABSTRACT FROM PUBLISHER]
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- 2015
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4. Ovine Lentivirus (Maedi-visna Virus) Protein Expression in Sheep Alveolar Macrophages
- Author
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Luján, L., primary, Begara, I., additional, Collie, D. D. S., additional, and Watt, N. J., additional
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- 1994
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5. Pathology of AA Amyloidosis in Domestic Sheep and Goats
- Author
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Ménsua, C., Carrasco, L., Bautista, M., Biescas, E., Fernández, A., Murphy, C., Weiss, D., Solomon, A., and Luján, L.
- Abstract
We describe the main pathologic changes in small ruminants affected by AA amyloidosis, together with the partial sequence of the protein involved. Twenty-one sheep and one goat were selected for presenting macroscopic kidney lesions compatible with systemic amyloidosis. Available tissue samples were studied by histologic, immunopathologic, and ultrastructural means. Renal lesions were characterized grossly by pale cortical surfaces with scattered, miliary, whitish-yellow foci and on cut cortical surfaces by straight, whitish-yellow striations. Gangrenous pneumonia was observed in 16 out of 21 affected sheep (76.2%), although other chronic inflammations were also observed. Amyloid was detected in all grossly affected kidneys using Congo red staining, lesions being most remarkable in glomeruli, affecting 95.5% of animals studied. Congophilic deposits were also observed in intertubular interstitium (68.2%) and medulla (57.1%). All amyloid-affected animals presented proximal convoluted tubule lesions, mostly characterized by an increase in diameter and by hyaline granular degeneration that were responsible for the macroscopic appearance of the kidney. Histologically, amyloid was also seen in blood vessels, spleen, liver, lymph nodes, gastrointestinal tract, and adrenal glands. All amyloid deposits demonstrated greenish-yellow birefringence with polarized light, and the antisera prepared against goat amyloid extracts specifically reacted with birefringent congophilic deposits of both sheep and goats. Ultrastructurally, these deposits were formed by masses of straight, nonbranching fibrils located predominantly in the basement membranes of glomerular capillaries and in the mesangium. Partial sequence of the protein in sheep and goats indicated a high degree of homology with the previously reported sequence of sheep Serum Amyloid A.
- Published
- 2003
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6. Mannheimia haemolytica -associated fibrinonecrotizing abomasitis in lambs.
- Author
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Pérez E, Uzal FA, de Miguel R, Rodríguez-Largo A, Reséndiz R, Streitenberger N, Macías-Rioseco M, Gómez Á, Calvo-Sánchez N, Pérez M, Luján L, and Asín J
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- Animals, Sheep, Stomach Diseases veterinary, Stomach Diseases pathology, Stomach Diseases microbiology, Male, Female, Immunohistochemistry veterinary, Mannheimia haemolytica isolation & purification, Sheep Diseases pathology, Sheep Diseases microbiology, Abomasum pathology, Abomasum microbiology, Pasteurellaceae Infections veterinary, Pasteurellaceae Infections pathology, Pasteurellaceae Infections microbiology, Necrosis veterinary, Necrosis pathology, Necrosis microbiology
- Abstract
Mannheimia haemolytica -associated abomasitis has been clinically described as a cause of sudden death in lambs, but it is poorly characterized. We describe the pathological features of a severe fibrinonecrotizing abomasitis in 3 lambs that died suddenly. All 3 abomasums had a thickened submucosa due to edema and necrotic areas delimited by bands of degenerate neutrophils with slender nuclei (oat cells) and angiocentric distributions. The overlying mucosa was congested. Myriads of gram-negative coccobacilli were observed within the oat cell bands. M. haemolytica was isolated from the abomasum in all 3 animals and was serotyped as A2 in one of them. Pericarditis and pleuritis were observed in 2 of the lambs. Clostridium spp. were isolated in 1 lamb and detected by immunohistochemistry in the 3 animals, suggesting clostridial co-infection. M. haemolytica should be considered among the differential diagnoses of necrotizing abomasitis in lambs., Competing Interests: Declaration of Conflicting InterestsThe author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.
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- 2024
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7. Photosensitization Induced by Heterophyllaea pustulata in Goats: Sequential Development of Skin Lesions.
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Micheloud JF, Luján L, Colque-Caro LA, Núñez-Montoya SC, Barbeito CG, Portiansky EL, and Gimeno EJ
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- Animals, Goats, Skin, Goat Diseases chemically induced, Photosensitivity Disorders chemically induced, Photosensitivity Disorders veterinary
- Abstract
Five adult Saanen goats received a single oral dose of Heterophyllaea pustulata containing 42.25 μg/kg rubiadin (anthraquinone) and 3 adult goats were untreated controls. All goats were exposed to sunlight and sequential ear skin biopsies were collected before treatment and at 32 hours, 3 days, 8 days, and 15 days after treatment. Changes at 32 hours after dosing included epidermal spongiosis, single cell death and acantholysis, an increased BAX/BCL-2 protein ratio, and dermal edema. Lesions at day 3 included epidermal and adnexal necrosis, crust formation, and acanthosis. Acanthosis, hyperkeratosis, and dermal fibrosis and neovascularization were present at day 15. The pro-apoptotic (BAX)/anti-apoptotic (BCL-2) protein ratio increased at 32 hours, whereas epidermal and dermal PCNA immunolabeling increased between days 8 and 15 after treatment. The cutaneous lesions were consistent with sunlight-induced damage, and the occurrence in treated but not control goats indicates photosensitization.
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- 2021
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8. Granulomas Following Subcutaneous Injection With Aluminum Adjuvant-Containing Products in Sheep.
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Asín J, Molín J, Pérez M, Pinczowski P, Gimeno M, Navascués N, Muniesa A, de Blas I, Lacasta D, Fernández A, de Pablo L, Mold M, Exley C, de Andrés D, Reina R, and Luján L
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- Adjuvants, Immunologic administration & dosage, Aluminum administration & dosage, Animals, Granuloma chemically induced, Granuloma pathology, Injection Site Reaction etiology, Injection Site Reaction pathology, Injections, Subcutaneous adverse effects, Injections, Subcutaneous veterinary, Lymph Nodes pathology, Male, Sheep, Sheep Diseases pathology, Adjuvants, Immunologic adverse effects, Aluminum adverse effects, Granuloma veterinary, Injection Site Reaction veterinary, Sheep Diseases chemically induced
- Abstract
The use of vaccines including aluminum (Al)-based adjuvants is widespread among small ruminants and other animals. They are associated with the appearance of transient injection site nodules corresponding to granulomas. This study aims to characterize the morphology of these granulomas, to understand the role of the Al adjuvant in their genesis, and to establish the presence of the metal in regional lymph nodes. A total of 84 male neutered lambs were selected and divided into 3 treatment groups of 28 animals each: (1) vaccine (containing Al-based adjuvant), (2) adjuvant-only, and (3) control. A total of 19 subcutaneous injections were performed in a time frame of 15 months. Granulomas and regional lymph nodes were evaluated by clinicopathological means. All of the vaccine and 92.3% of the adjuvant-only lambs presented injection-site granulomas; the granulomas were more numerous in the group administered the vaccine. Bacterial culture in granulomas was always negative. Histologically, granulomas in the vaccine group presented a higher degree of severity. Al was specifically identified by lumogallion staining in granulomas and lymph nodes. Al median content was significantly higher ( P < .001) in the lymph nodes of the vaccine group (82.65 μg/g) compared with both adjuvant-only (2.53 μg/g) and control groups (0.96 μg/g). Scanning transmission electron microscopy demonstrated aggregates of Al within macrophages in vaccine and adjuvant-only groups. In these two groups, Al-based adjuvants induce persistent, sterile, subcutaneous granulomas with macrophage-driven translocation of Al to regional lymph nodes. Local translocation of Al may induce further accumulation in distant tissues and be related to the appearance of systemic signs.
- Published
- 2019
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9. Congenital Hepatic Fibrosis in a Purebred Spanish Horse Foal: Pathology and Genetic Studies on PKHD1 Gene Mutations.
- Author
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Molín J, Asín J, Vitoria A, Sanz A, Gimeno M, Romero A, Sánchez J, Pinczowski P, Vázquez FJ, Rodellar C, and Luján L
- Subjects
- Animals, Fatal Outcome, Genetic Diseases, Inborn genetics, Genetic Diseases, Inborn pathology, Genotype, Horse Diseases genetics, Horse Diseases pathology, Horses, Liver pathology, Liver Cirrhosis congenital, Liver Cirrhosis genetics, Liver Cirrhosis pathology, Polymorphism, Single Nucleotide, Receptors, Cell Surface genetics, Genetic Diseases, Inborn veterinary, Horse Diseases congenital, Liver Cirrhosis veterinary, Receptors, Cell Surface metabolism
- Abstract
A 1-month-old Purebred Spanish Horse (PSH) foal presented with progressive hepatic failure culminating in death. Hepatic lesions were consistent with congenital hepatic fibrosis (CHF). Genetic studies in the PKHD1 gene in the affected foal revealed that it was heterozygous for the 2 previously described single-nucleotide polymorphisms (SNPs) linked to CHF in Swiss Franches-Montagnes (SFM) horses. In addition, 2 novel mutations were detected, the foal being homozygous for one of them and heterozygous for the other. Genetic studies in a healthy PSH population ( n = 35) showed a 3-fold higher genotypic frequency for PKHD1 SNP g.49,630,834G>A and a 5-fold higher genotypic frequency for PKHD1 SNP g.49,597,760A>T compared with those reported for SFM horses. SNPs in the PKHD1 gene in CHF-affected SFM horses might not fully explain the CHF observed in the PSH. Other mutations in the PKHD1 gene could play a more important role in the PSH.
- Published
- 2018
- Full Text
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