9 results on '"Hilbe M"'
Search Results
2. Apoptosis in Bovine Viral Diarrhea Virus (BVDV)–Induced Mucosal Disease Lesions
- Author
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Hilbe, M., primary, Girao, V., additional, Bachofen, C., additional, Schweizer, M., additional, Zlinszky, K., additional, and Ehrensperger, F., additional
- Published
- 2012
- Full Text
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3. Distribution of Borna Disease Virus Antigen and RNA in Tissues of Naturally Infected Bicolored White-Toothed Shrews, Crocidura leucodon, Supporting Their Role as Reservoir Host Species
- Author
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Puorger, M. E., primary, Hilbe, M., additional, Müller, J.-P., additional, Kolodziejek, J., additional, Nowotny, N., additional, Zlinszky, K., additional, and Ehrensperger, F., additional
- Published
- 2010
- Full Text
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4. Metastatic Calcification in a Dog Attributable to Ingestion of a Tacalcitol Ointment
- Author
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Hilbe, M., primary, Sydler, T., additional, Fischer, L., additional, and Naegeli, H., additional
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- 2000
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5. Apoptosis in Bovine Viral Diarrhea Virus (BVDV)–Induced Mucosal Disease Lesions: A Histological, Immunohistological, and Virological Investigation.
- Author
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Hilbe, M., Girao, V., Bachofen, C., Schweizer, M., Zlinszky, K., and Ehrensperger, F.
- Subjects
APOPTOSIS ,BOVINE viral diarrhea ,CATTLE diseases ,IMMUNOHISTOCHEMISTRY ,MUCOSAL diseases in cattle ,CELL death - Abstract
Cattle persistently infected with a noncytopathic Bovine viral diarrhea virus (BVDV) are at risk of developing fatal “mucosal disease” (MD). The authors investigated the role of various apoptosis pathways in the pathogenesis of lesions in animals suffering from MD. Therefore, they compared the expression of caspase-3, caspase-8, caspase-9, and Bcl-2L1 (Bcl-x) in tissues of 6 BVDV-free control animals, 7 persistently infected (PI) animals that showed no signs of MD (non-MD PI animals), and 11 animals with MD and correlated the staining with the localization of mucosal lesions. Caspase-3 and -9 staining were markedly stronger in MD cases and were associated with mucosal lesions, even though non-MD PI animals and negative controls also expressed caspase-9. Conversely, caspase-8 was not elevated in any of the animals analyzed. Interestingly, Bcl-x also colocalized with mucosal lesions in the MD cases. However, Bcl-x was similarly expressed in tissues from all 3 groups, and thus, its role in apoptosis needs to be clarified. This study clearly illustrates ex vivo that the activation of the intrinsic, but not the extrinsic, apoptosis pathway is a key element in the pathogenesis of MD lesions observed in cattle persistently infected with BVDV. However, whether direct induction of apoptosis in infected cells or indirect effects induced by the virus are responsible for the lesions observed remains to be established. [ABSTRACT FROM PUBLISHER]
- Published
- 2013
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6. Exudative glomerulonephritis associated with acute leptospirosis in dogs.
- Author
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Hilbe M, Posthaus H, Paternoster G, Schuller S, Imlau M, and Jahns H
- Subjects
- Animals, Dogs, Retrospective Studies, Male, Female, Switzerland, Immunohistochemistry veterinary, Leptospira interrogans isolation & purification, Acute Disease, Polymerase Chain Reaction veterinary, Antibodies, Bacterial blood, Leptospirosis veterinary, Leptospirosis pathology, Leptospirosis diagnosis, Leptospirosis microbiology, Dog Diseases pathology, Dog Diseases microbiology, Dog Diseases diagnosis, Glomerulonephritis veterinary, Glomerulonephritis pathology, Glomerulonephritis microbiology
- Abstract
In the past 20 years in Switzerland, dogs with suspect acute leptospirosis frequently showed severe glomerular changes that had not been previously reported. These features were characterized by abundant extravasated erythrocytes and fewer neutrophils accompanied by marked fibrin exudation into the urinary space that was interpreted as an exudative glomerulonephritis (GN). This retrospective study describes this significant glomerular pathological change and investigates the association with leptospirosis. Tissues from 50 dogs with exudative GN, retrieved from 2 pathology archives in Switzerland were reviewed using hematoxylin and eosin, periodic acid-Schiff, phosphotungstic acid-hematoxylin, and Warthin and Starry stains. Clinical and postmortem data were collected for each case. Immunohistochemistry (IHC) and/or polymerase chain reactions were used as confirmatory tests for leptospirosis. While all 50 cases had clinical and pathological features supporting a diagnosis of leptospirosis, 37 cases were confirmed for the disease. Using a LipL32 antibody in addition to the OMV2177 antibody raised against the lipopolysaccharide of Leptospira interrogans serovar Copenhageni increased the detection rate of Leptospira by IHC in exudative GN from 24% to 62%. Signalment, seasonality, clinical signs, blood results, and pathological changes in dogs with exudative GN were similar to those reported for dogs without GN and confirmed infection by Leptospira spp.. Exudative GN was common among Swiss dogs with leptospirosis where it caused acute severe disease. Leptospirosis should be considered as a cause of this new pathologic feature by the pathologist. The pathogenesis remains unclear, but involvement of a geographic-specific serovar with unique virulence factors is suspected and warrants further investigation., Competing Interests: Declaration of Conflicting InterestsThe author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.
- Published
- 2024
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7. Chlamydia pecorum -Induced Arthritis in Experimentally and Naturally Infected Sheep.
- Author
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Ostfeld N, Islam MM, Jelocnik M, Hilbe M, Sydler T, Hartnack S, Jacobson C, Clune T, Marsh I, Sales N, Polkinghorne A, and Borel N
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- Animals, Cattle, Sheep, Sheep, Domestic, Swine, Arthritis, Infectious veterinary, Cattle Diseases, Chlamydia, Chlamydia Infections veterinary, Phascolarctidae, Sheep Diseases, Swine Diseases
- Abstract
Chlamydia pecorum is an obligate intracellular pathogen with a wide host range including livestock such as sheep, cattle, goats, and pigs as well as wildlife species such as koalas. Chlamydial polyarthritis is an economically important disease resulting in swollen joints, lameness, stiffness, and weight loss in young sheep. In the present study, tissues from sheep experimentally or naturally infected with Chlamydia pecorum were assessed by histopathology and immunohistochemistry. Carpal, hock, and stifle joints as well as spleen, liver, kidney, lymph nodes, lung, and brain of 35 sheep from different inoculation groups were available. Two different C. pecorum strains (IPA and E58), different routes of administration (intraarticular or intravenous), UVA-irradiated IPA strain, and corresponding noninfected control groups were investigated. Similar investigations on tissues from 5 naturally infected sheep were performed. The most obvious inflammatory lesions were observed in synovial tissues and, notably, in the renal pelvis from the experimentally infected group and naturally infected animals. This resulted in chronic or chronic-active arthritis and pyelitis. Intralesional chlamydial inclusions could be demonstrated by immunohistochemistry in both tissues. Immunohistochemical evaluation of the presence and distribution of macrophages, T and B cells in synovial tissues revealed macrophages as the most prevalent inflammatory cell population. Previous observations indicated that C. pecorum isolates can infect circulating monocytes. Together with the finding of the histological lesions in synovial tissues and internal organs alongside the presence of C. pecorum DNA, these observations suggest chlamydial arthritis in lambs is the result of hematogeneous spread of C. pecorum .
- Published
- 2021
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8. Bovine Fetal Placenta During Pregnancy and the Postpartum Period.
- Author
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Botta C, Pellegrini G, Hässig M, Pesch T, Prähauser B, Wunderlin S, Guscetti F, Schneeberger M, Schmitt S, Basso W, Hilbe M, Schuler G, and Borel N
- Subjects
- Animals, Caspase 3 metabolism, Cattle, Chlamydiaceae, Coxiella burnetii, Female, Lamin Type A metabolism, Neospora, Placenta microbiology, Placenta ultrastructure, Postpartum Period, Pregnancy, Real-Time Polymerase Chain Reaction, Placenta anatomy & histology
- Abstract
Bovine abortion is a worldwide problem, but despite extensive histopathologic and molecular investigations, the cause of abortion remains unclear in about 70% of cases. Cellular debris is a commonly observed histopathologic finding in the fetal placenta and is often interpreted as necrosis. In this study, the nature of this cellular debris was characterized, and histologic changes in the normal fetal placenta during pregnancy and after delivery were assessed. In addition, the presence of the most common abortifacient pathogens in Switzerland ( Chlamydiaceae, Coxiella burnetii, Neospora caninum) was tested by polymerase chain reaction. We collected 51 placentomes and 235 cotyledons from 41 and from 50 cows, respectively. In total, cellular debris was present in 48 of 51 (94%) placentomes and in 225 of 235 (96%) cotyledons, inflammation occurred in 1 of 51 (2%) placentomes and in 46 of 235 (20%) cotyledons, vasculitis was seen in 1 of 51 (2%) placentomes and 46 of 235 (20%) cotyledons, and 18 of 51 (35%) placentomes and 181 of 235 (77%) cotyledons had mineralization. The amount of cellular debris correlated with areas of positive signals for cleaved caspase 3 and lamin A. Therefore, this finding was interpreted as an apoptotic process. In total, 10 of 50 cotyledons (20%) were positive for C. burnetii DNA, most likely representing subclinical infections. The results of our study indicate that histologic features in the fetal placenta such as cellular debris, inflammation, vasculitis, and mineralization must be considered physiological processes during pregnancy and after delivery. Therefore, their presence in placentae of aborted fetuses must be interpreted with caution and might not be necessarily linked to an infectious cause of abortion.
- Published
- 2019
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9. Border Disease Virus Infection of Bovine Placentas.
- Author
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Fernández M, Braun U, Frei S, Schweizer M, and Hilbe M
- Subjects
- Animals, Border Disease virology, Cattle, Cattle Diseases pathology, Female, Fetus virology, Infectious Disease Transmission, Vertical veterinary, Placenta Diseases virology, Pregnancy, Sheep, Border Disease pathology, Border disease virus isolation & purification, Cattle Diseases virology, Placenta virology, Placenta Diseases veterinary
- Abstract
Subsequent to a previous study of border disease virus (BDV) horizontal transmission from a persistently BDV-infected calf to 6 seronegative pregnant heifers, the heifers were slaughtered 60 days after exposure to the infected calf, and their fetuses and placentas were examined. Immunohistochemical examination of fetal organs and placenta showed positive labeling of moderate intensity for pestivirus antigen in 3 of 6 heifers. BDV infection in these 3 animals was confirmed by the detection of BDV RNA in different organs using reverse transcription quantitative polymerase chain reaction. In the placenta, the positive cells were visualized mostly on the fetal side. In those 3 heifers that harbored an infected fetus, the placental tissue in the placentome region showed a moderate to severe mononuclear and fibrosing placentitis and, in severe cases, necrotic areas. The inflammatory population was composed predominantly of T and B cells, a substantial number of macrophages, and, to a lesser extent, plasma cells. This is a novel report of placentitis in persistently BDV-infected fetuses from pregnant heifers that became acutely infected by cohousing with a calf persistently infected with BDV, which extends previous reports on bovine viral diarrhea virus-infected and BDV-infected cattle and sheep, respectively.
- Published
- 2018
- Full Text
- View/download PDF
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