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Start Over Author "Tong, Wu" Journal veterinary microbiology
17 results on '"Tong, Wu"'

4. KLF16 inhibits PEDV replication by activating the type I IFN signaling pathway

Author

Dong, Sujie, primary, Kong, Ning, additional, Shen, Haiyan, additional, Li, Youwen, additional, Qin, Wenzhen, additional, Zhai, Huanjie, additional, Zhai, Xueying, additional, Yang, Xinyu, additional, Ye, Chenqian, additional, Ye, Manqing, additional, Liu, Changlong, additional, Yu, Lingxue, additional, Zheng, Hao, additional, Tong, Wu, additional, Yu, Hai, additional, Zhang, Wen, additional, Tong, Guangzhi, additional, and Shan, Tongling, additional

Published
2022
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5. ATG4B hinders porcine epidemic diarrhea virus replication through interacting with TRAF3 and activating type-I IFN signaling

Author

Dong, Sujie, primary, Kong, Ning, additional, Qin, Wenzhen, additional, Zhai, Huanjie, additional, Zhai, Xueying, additional, Yang, Xinyu, additional, Ye, Chenqian, additional, Ye, Manqing, additional, Liu, Changlong, additional, Yu, Lingxue, additional, Zheng, Hao, additional, Tong, Wu, additional, Yu, Hai, additional, Zhang, Wen, additional, Li, Youwen, additional, Tong, Guangzhi, additional, and Shan, Tongling, additional

Published
2022
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8. Pseudorabies virus pUL16 assists the nuclear import of VP26 through protein-protein interaction

Author

Xu, Jing-jing, primary, Cheng, Xue-fei, additional, Wu, Ji-qiang, additional, Zheng, Hao, additional, Tong, Wu, additional, Chen, Xiaoyong, additional, Ye, Chao, additional, Liu, Yuting, additional, Zhu, Haojie, additional, Fu, Xinling, additional, Jiang, Yifeng, additional, Kong, Ning, additional, Tong, Guangzhi, additional, Gao, Fei, additional, and Li, Guoxin, additional

Published
2021
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9. The role of PA-X C-terminal 20 residues of classical swine influenza virus in its replication and pathogenicity

Author

Wang, Xiu-Hui, primary, Gong, Xiao-Qian, additional, Wen, Feng, additional, Ruan, Bao-Yang, additional, Yu, Ling-Xue, additional, Liu, Xiao-Min, additional, Wang, Qi, additional, Wang, Shuai-Yong, additional, Wang, Juan, additional, Zhang, Yi-Feng, additional, Zhou, Yan-Jun, additional, Shan, Tong-Ling, additional, Tong, Wu, additional, Zheng, Hao, additional, Kong, Ning, additional, Yu, Hai, additional, and Tong, Guang-Zhi, additional

Published
2020
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10. Evaluation of immune efficacy of recombinant PRRSV vectored vaccine rPRRSV-E2 in piglets with maternal derived antibodies

Author

Gao, Fei, primary, Jiang, Yifeng, additional, Li, Guoxin, additional, Li, Liwei, additional, Zhang, Yujiao, additional, Yu, Lingxue, additional, Zheng, Hao, additional, Tong, Wu, additional, Zhou, Yanjun, additional, Liu, Changlong, additional, Shan, Tongling, additional, Yu, Hai, additional, Kong, Ning, additional, Chen, Pengfei, additional, and Tong, Guangzhi, additional

Published
2020
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11. Caveolae/rafts protect human cerebral microvascular endothelial cells from Streptococcus suis serotype 2 α-enolase-mediated injury

Author

Tong Wu, Jingmin Gu, Hexiang Jiang, Jianan Liu, Na Li, Xin Feng, Yalu Ji, Xibing Yu, Mengmeng Liu, Liancheng Lei, Chuntong Bao, Wenyu Han, and Hongtao Liu

Subjects
Streptococcus suis, Virulence Factors, Caveolin 1, Cell, Fluorescent Antibody Technique, Biology, Caveolae, Serogroup, Microbiology, Virulence factor, Cell Line, Flow cytometry, Pathogenesis, 03 medical and health sciences, medicine, Animals, Humans, 030304 developmental biology, Ribosome Subunits, Small, Eukaryotic, 0303 health sciences, General Veterinary, medicine.diagnostic_test, 030306 microbiology, Endothelial Cells, General Medicine, Cell biology, Blot, HEK293 Cells, medicine.anatomical_structure, Phosphopyruvate Hydratase, Intracellular
Abstract

Streptococcus suis serotype 2 (SS2) is an important zoonotic pathogen that causes meningitis. The ubiquitously expressed 40S ribosome protein SA (RPSA) is a multifunctional protein involved in the pathogenesis of multiple pathogens, especially those causing meningitis. However, the role of RPSA in SS2-induced meningitis is not clear. In this study, immunofluorescence staining revealed that SS2 infection promoted the intracellular transfer of RPSA to the surface of human cerebral microvascular endothelial cells (HCMECs). Moreover, SS2 infection promoted the accumulation of caveolin 1 (CAV1) and the formation of membrane bulges where RPSA enveloped CAV1 on the cell surface. SS2 infection also caused dynamic changes in the localization of RPSA and CAV1 on the cell surface which could be eliminated by disruption of caveolae/rafts by addition of methyl-β-cyclodextrin (MβCD). Co-immunoprecipitation analysis demonstrated that α-enolase (ENO), a key virulence factor of SS2, interacted with RPSA, and promoted the interaction between RPSA and CAV1. Immunofluorescence staining, western blotting and flow cytometry analyses showed that damaged caveolae/rafts significantly enhanced ENO adhesion to HCMECs, promoted the "destruction" of RPSA by ENO, and enhanced the toxic effect of ENO on HCMECs. Importantly, these effects could be relieved upon the addition of cholesterol. We conclude that caveolae/rafts weaken the toxic effect of SS2 ENO on RPSA-mediated events in HCMECs. Our study has led to better understanding of the roles of RPSA and caveolae/rafts upon SS2 infection, and a new pathological role for RPSA in infection.

Published
2021

14. PA-X protein decreases replication and pathogenicity of swine influenza virus in cultured cells and mouse models

Author

Gong, Xiao-Qian, primary, Sun, Ying-Feng, additional, Ruan, Bao-Yang, additional, Liu, Xiao-Min, additional, Wang, Qi, additional, Yang, Hai-Ming, additional, Wang, Shuai-Yong, additional, Zhang, Peng, additional, Wang, Xiu-Hui, additional, Shan, Tong-Ling, additional, Tong, Wu, additional, Zhou, Yan-Jun, additional, Li, Guo-Xin, additional, Zheng, Hao, additional, Tong, Guang-Zhi, additional, and Yu, Hai, additional

Published
2017
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16. Protective efficacy of a high-growth reassortant H1N1 influenza virus vaccine against the European Avian-like H1N1 swine influenza virus in mice and pigs.

Author

Ruan, Bao-Yang, Wen, Feng, Gong, Xiao-Qian, Liu, Xiao-Min, Wang, Qi, Yu, Ling-Xue, Wang, Shuai-Yong, Zhang, Peng, Yang, Hai-Ming, Shan, Tong-Ling, Zheng, Hao, Zhou, Yan-Jun, Tong, Wu, Gao, Fei, Tong, Guang-Zhi, and Yu, Hai

Subjects
*INFLUENZA A virus, H1N1 subtype, *VIRAL vaccines, *SWINE influenza, *IMMUNOGLOBULINS, *BLOOD agglutination, *PREVENTION
Abstract

Swine influenza A viruses (SIVs) causing outbreaks of acute, highly contagious respiratory disease in pigs also pose a potential threat to public health. European avian-like H1N1 (EA H1N1) SIVs are the predominant circulating viruses in pigs in China and also occasionally cause human infection. In this study, a high-growth reassortant virus (SH1/PR8), with HA and NA genes from a representative EA H1N1 isolate A/Swine/Shanghai/1/2014 (SH1) in China and six internal genes from the high-growth A/Puerto Rico/8/34 (PR8) virus, was generated by plasmid-based reverse genetics and tested as a candidate seed virus for the preparation of inactivated vaccine. The protective efficacy of inactivated SH1/PR8 was evaluated in mice and pigs challenged with wild-type SH1 virus. After primer and boost vaccination, the SH1/PR8 vaccine induced high-level hemagglutination inhibiting (HI) antibodies, IgG antibodies, and neutralization antibodies in mice and pigs. Mice and pigs in the vaccinated group showed less clinical phenomena and pathological changes than those in the unvaccinated group. In conclusion, the inactivated high-growth reassortant vaccine SH1/PR8 could induce high antibody levels and complete protection is expected against SH1 wild type SIV, and protection against heterologous EA H1N1 SIV needs further evaluation. [ABSTRACT FROM AUTHOR]

Published
2018
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17. Protective efficacy of a bivalent inactivated reassortant H1N1 influenza virus vaccine against European avian-like and classical swine influenza H1N1 viruses in mice.

Author

Ruan, Bao-Yang, Yao, Yun, Wang, Shuai-Yong, Gong, Xiao-Qian, Liu, Xiao-Min, Wang, Qi, Yu, Ling-Xue, Zhu, Shi-Qiang, Wang, Juan, Shan, Tong-Ling, Zhou, Yan-Jun, Tong, Wu, Zheng, Hao, Li, Guo-Xin, Gao, Fei, Kong, Ning, Yu, Hai, and Tong, Guang-Zhi

Subjects
*H1N1 influenza, *SWINE influenza, *INFLUENZA vaccines, *INFLUENZA A virus, H1N1 subtype, *INFLUENZA viruses
Abstract

• Classical swine (CS) and European avian-like (EA) H1N1 subtype SIVs are circulating in pigs in China. • A bivalent reassortant inactivated vaccine comprised of SH1/PR8 and G11/PR8 was developed. • The vaccine could provide complete protection against the prevalent EA and CS H1N1 subtype SIVs. The classical swine (CS) H1N1 swine influenza virus (SIVs) emerged in humans as a reassortant virus that caused the H1N1 influenza virus pandemic in 2009, and the European avian-like (EA) H1N1 SIVs has caused several human infections in European and Asian countries. Development of the influenza vaccines that could provide effective protective efficacy against SIVs remains a challenge. In this study, the bivalent reassortant inactivated vaccine comprised of SH1/PR8 and G11/PR8 arboring the hemagglutinin (HA) and neuraminidase (NA) genes from prevalent CS and EA H1N1 SIVs and six internal genes from the A/Puerto Rico/8/34(PR8) virus was developed. The protective efficacy of this bivalent vaccine was evaluated in mice challenged with the lethal doses of CS and EA H1N1 SIVs. The result showed that univalent inactivated vaccine elicited high-level antibody against homologous H1N1 viruses while cross-reactive antibody responses to heterologous H1N1 viruses were not fully effective. In a mouse model, the bivalent inactivated vaccine conferred complete protection against lethal challenge doses of EA SH1 virus or CS G11 virus, whereas the univalent inactivated vaccine only produced insufficient protection against heterologous SIVs. In conclusion, our data demonstrated that the reassortant bivalent inactivated vaccine comprised of SH1/PR8 and G11/PR8 could provide effective protection against the prevalent EA and CS H1N1 subtype SIVs in mice. [ABSTRACT FROM AUTHOR]

Published
2020
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