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3. Molecular characterisation of methicillin-resistant and methicillin-susceptible Staphylococcus aureus clones isolated from healthy dairy animals and their caretakers in Egypt

Author

El-Ashker, Maged, primary, Monecke, Stefan, additional, Gwida, Mayada, additional, Saad, Thoraya, additional, El-Gohary, Adel, additional, Mohamed, Amro, additional, Reißig, Annett, additional, Frankenfeld, Katrin, additional, Gary, Dominik, additional, Müller, Elke, additional, and Ehricht, Ralf, additional

Published
2022
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8. Characterization of mecC gene-carrying coagulase-negative Staphylococcus spp. isolated from various animals

Author

Loncarić, Igor, Kuebber-Heiss, Anna, Posautz, Annika, Ruppitsch, Werner, Lepuschitz, Sarah, Schauer, Bernhard, Fessler, Andrea T., Krametter-Froetscher, Reinhild, Harrison, Ewan M., Holmes, Mark A., Kuenzel, Frank, Szostak, Michael, Hauschild, Tomasz, Desvars-Larrive, Amelie, Mišić, Dušan, Rosengarten, Renate, Walzer, Chris, Slickers, Peter, Monecke, Stefan, Ehricht, Ralf, Schwarz, Stefan, Spergser, Joachim, Loncarić, Igor, Kuebber-Heiss, Anna, Posautz, Annika, Ruppitsch, Werner, Lepuschitz, Sarah, Schauer, Bernhard, Fessler, Andrea T., Krametter-Froetscher, Reinhild, Harrison, Ewan M., Holmes, Mark A., Kuenzel, Frank, Szostak, Michael, Hauschild, Tomasz, Desvars-Larrive, Amelie, Mišić, Dušan, Rosengarten, Renate, Walzer, Chris, Slickers, Peter, Monecke, Stefan, Ehricht, Ralf, Schwarz, Stefan, and Spergser, Joachim

Abstract

The presence of the methicillin resistance gene mecC in coagulase-negative Staphylococcus spp. (CoNS) is scarce. The aim of this study was to characterize mecC-positive CoNS isolated from various wild and domestic animals. The presence of the mecC gene was screened in 4299 samples from wild animals and domestic animals. Fifteen coagulase-negative staphylococci, that displayed a cefoxitin-resistant phenotype, were tested mecC-positive by PCR. Antimicrobial susceptibility testing was performed for all isolates. The 15 isolates were genotyped by sequencing of the entire class E mec gene complex (blaZ-mecC-mecRI-mecI), the ccrA and ccrB recombinase genes and other determinants within the type XI SCCmec element. DNA microarray analysis was performed and five selected isolates were additionally whole genome sequenced and analyzed. S. stepanovicii (n = 3), S. caprae (n = 1), S. warneri (n = 1), S. xylosus (n = 1) and S. sciuri (n = 9) were detected. All but the S. sciuri isolates were found to be susceptible to all non-beta lactams. The entire class E mec gene complex was detected in all isolates but ccrA and ccrB genes were not identified in S. stepanovicii and S. xylosus. The genes erm(B) and fexA (n = 4, each) were the most predominant non-beta lactam resistance genes detected in the S. sciuri isolates. Even though the presence of the mecC gene among CoNS is a rare observation, this study further expands our knowledge by showing that the mecC gene, including its allotypes, are present in more staphylococcal species from different animal species than has been previously described.

Published
2019

9. Increased genetic diversity of methicillin-resistant Staphylococcus aureus (MRSA) isolated from companion animals

Author

Loncaric, Igor, primary, Lepuschitz, Sarah, additional, Ruppitsch, Werner, additional, Trstan, Alan, additional, Andreadis, Thomas, additional, Bouchlis, Nikolaos, additional, Marbach, Helene, additional, Schauer, Bernhard, additional, Szostak, Michael P., additional, Feßler, Andrea T., additional, Künzel, Frank, additional, Licka, Theresia, additional, Springer, Burkhard, additional, Allerberger, Franz, additional, Monecke, Stefan, additional, Ehricht, Ralf, additional, Schwarz, Stefan, additional, and Spergser, Joachim, additional

Published
2019
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11. Characterization of mecC gene-carrying coagulase-negative Staphylococcus spp. isolated from various animals

Author

Loncaric, Igor, primary, Kübber-Heiss, Anna, additional, Posautz, Annika, additional, Ruppitsch, Werner, additional, Lepuschitz, Sarah, additional, Schauer, Bernhard, additional, Feßler, Andrea T., additional, Krametter-Frötscher, Reinhild, additional, Harrison, Ewan M., additional, Holmes, Mark A., additional, Künzel, Frank, additional, Szostak, Michael P., additional, Hauschild, Tomasz, additional, Desvars-Larrive, Amélie, additional, Misic, Dusan, additional, Rosengarten, Renate, additional, Walzer, Chris, additional, Slickers, Peter, additional, Monecke, Stefan, additional, Ehricht, Ralf, additional, Schwarz, Stefan, additional, and Spergser, Joachim, additional

Published
2019
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13. Variability of SCCmec elements in livestock-associated CC398 MRSA

Author

Monecke, Stefan, primary, Slickers, Peter, additional, Gawlik, Darius, additional, Müller, Elke, additional, Reissig, Annett, additional, Ruppelt-Lorz, Antje, additional, de Jäckel, Sonia Cortez, additional, Feßler, Andrea T., additional, Frank, Martina, additional, Hotzel, Helmut, additional, Kadlec, Kristina, additional, Jatzwauk, Lutz, additional, Loncaric, Igor, additional, Schwarz, Stefan, additional, Schlotter, Katharina, additional, Thürmer, Alexander, additional, Wendlandt, Sarah, additional, and Ehricht, Ralf, additional

Published
2018
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15. Immune response of Staphylococcus aureus strains in a mouse mastitis model is linked to adaptive capacity and genotypic profiles

Author

Andrea Belén Duré, Stefan Monecke, Luis Fernando Calvinho, Maria Sol Renna, Celina Baravalle, Carolina Soledad Andreotti, Elizabet Amanda Lorena Pereyra, Sofía Clara Sacco, and Bibiana Elisabet Dallard

Subjects
0301 basic medicine, Staphylococcus aureus, Genotype, 040301 veterinary sciences, Inflammation, Mastitis, Biology, medicine.disease_cause, Microbiology, 0403 veterinary science, Pathogenesis, 03 medical and health sciences, Mice, Immune system, PERSISTENT AND NON-PERSISTENT STRAINS, IMMUNE RESPONSE, Otras Ciencias Veterinarias, MASTITIS, medicine, Animals, STAPHYLOCOCCUS AUREUS, Pathogen, MICE MODEL, General Veterinary, Strain (chemistry), Ciencias Veterinarias, 04 agricultural and veterinary sciences, General Medicine, medicine.disease, Adaptation, Physiological, TLR2, 030104 developmental biology, CIENCIAS AGRÍCOLAS, Immunology, Female, medicine.symptom
Abstract

Staphylococcus aureus is one of the most frequently isolated major pathogens from intramammary infections (IMI) worldwide. The mechanisms by which S. aureus IMI are established and maintained in dairy cows involve both bacterial escape strategies and modulation of the host immune response. Moreover, it was shown that different S. aureus strains have varying effects on the immune response. The aim of this study was to investigate the immune response in a mouse mastitis model of two S. aureus strains isolated from bovine IMI with different clinical manifestation (persistent-P or non-persistent-NP), phenotypic and genotypic profile. Both strains were capable of establishing an IMI after 264 h post inoculation (pi). Strain A (NP) showed a more aggressive behaviour than strain B (P) at early stages of IMI, while strain B multiplied initially at a lower rate but increased its replication capacity from 120 h pi to the end of the study (264 h pi). Strain A triggered a stronger initial inflammatory response compared with strain B inducing higher gene and protein expression of TLR2, NF-κB activation and higher gene expression of IL-1α at initial stage of IMI (6?12 h pi) but inducing extensive mammary tissue damage. Immune cells response was different for each S. aureus strain throughout the course of infection, showing mammary glands inoculated with strain A greater initial immune cells stimulation compared with strain B and then a second immune cells stimulation (from 120 to 264 h pi) represented by monocytes-macrophages, T and B lymphocytes, mainly stimulated by strain B, consistent with inflammatory process becoming chronic. Strain-specific pathogenicity observed underscores the importance of pathogen factors in the progression of the infectious process. These results contribute to increase the available information on host-pathogen interaction and point out for the need of further research to expand the knowledge about these interactions for developing new strategies to intervene in the IMI progress. Fil: Pereyra, Elizabet Amanda Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina Fil: Sacco, Sofía Clara. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina Fil: Duré, Andrea Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina Fil: Baravalle, Celina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina Fil: Renna, Maria Sol. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina Fil: Andreotti, Carolina Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina Fil: Monecke, Stefan. Technische Universität Dresden; Alemania. Alere Technologies GmbH; Alemania Fil: Calvinho, Luis Fernando. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Clínicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela; Argentina Fil: Dallard, Bibiana Elisabet. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina

Published
2016

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