4 results on '"Lauzi, Stefania"'
Search Results
2. Feline morbillivirus in Northern Italy: prevalence in urine and kidneys with and without renal disease.
- Author
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Stranieri, Angelica, Lauzi, Stefania, Dallari, Annachiara, Gelain, Maria Elena, Bonsembiante, Federico, Ferro, Silvia, and Paltrinieri, Saverio
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KIDNEY diseases , *KIDNEYS , *URINE , *UNIVERSITY hospitals , *VETERINARY hospitals - Abstract
• Only 2/27 kidneys and 1/81 urine samples were FeMV PCR positive. • All cats with chronic kidney disease were FeMV PCR negative on urine. • Only 1/14 cats with proteinuria was FeMV positive on urine. • The 2 FeMV PCR positive kidneys were from cats affected by FIP and adenocarcinoma. • Italian FeMV strains form a sub-cluster with strains isolated from other countries. Feline morbillivirus (FeMV) is an emerging virus that was first described in Hong Kong in 2012. Several reports suggested the epidemiological association of FeMV infection with chronic kidney disease (CKD) in cats. The aim of this study was to investigate the presence and the genetic diversity of FeMV as well as the relationship between FeMV infection and CKD in cats from Northern Italy. Urine (n = 81) and kidney samples (n = 27) from 92 cats admitted to the Veterinary Teaching Hospital of the University of Milan between 2014 and 2017 were investigated for FeMV infection. FeMV RNA was detected in one urine sample (1.23%; 95% CI: 0.03–6.68%) and in two kidneys (7.40%; 95% CI: 0.91–24.28%). FeMV RNA was revealed only in urine or kidneys of cats without evidence of CKD. Phylogenetic analysis showed that the three strains clustered with FeMV strains retrieved from public database, forming a distinct sub-cluster of FeMV. The presence of distinct genotypes of FeMV found in this study is in accordance with previous studies demonstrating that FeMV strains are genetically diverse. A clear relationship between the presence of FeMV infection and CKD in the cats from Northern Italy was not observed, confirming recent reports that do not support the hypothesis that FeMV infection is associated with the development of CKD. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
3. Host range of mammalian orthoreovirus type 3 widening to alpine chamois.
- Author
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Besozzi, Martina, Lauzi, Stefania, Lelli, Davide, Lavazza, Antonio, Chiapponi, Chiara, Pisoni, Giuliano, Viganò, Roberto, Lanfranchi, Paolo, and Luzzago, Camilla
- Subjects
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CHAMOIS , *ORTHOREOVIRUSES , *VETERINARY virology , *NUCLEOTIDE sequencing , *LUNG microbiology - Abstract
Highlights • MRV-3 has been identified from lung tissues of alpine chamois in Italian Alps. • Virus belongs to lineage III clustering with strains from dog, bat and diarrheic pig. • Whole genome sequence highlighted reassortment and lack of host specific barriers. • No respiratory symptoms neither lung macroscopic lesions were observed. • High seroprevalence was observed in chamois population during a five-years survey. Abstract Mammalian orthoreoviruses (MRV) type 3 have been recently identified in human and several animal hosts, highlighting the apparent lack of species barriers. Here we report the identification and genetic characterization of MRVs strains in alpine chamois, one of the most abundant wild ungulate in the Alps. Serological survey was also performed by MRV neutralization test in chamois population during five consecutive years (2008-2012). Three novel MRVs were isolated on cell culture from chamois lung tissues. No respiratory or other clinical symptoms neither lung macroscopic lesions were observed in the chamois population. MRV strains were classified as MRV-3 within the lineage III, based on S1 phylogeny, and were closely related to Italian strains identified in dog, bat and diarrheic pig. The full genome sequence was obtained by next-generation sequencing and phylogenetic analyses showed that other segments were more similar to MRVs of different geographic locations, serotypes and hosts, including human, highlighting genome reassortment and lack of host specific barriers. By using serum neutralization test, a high prevalence of MRV-3 antibodies was observed in chamois population throughout the monitored period, showing an endemic level of infection and suggesting a self-maintenance of MRV and/or a continuous spill-over of infection from other animal species. [ABSTRACT FROM AUTHOR]
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- 2019
- Full Text
- View/download PDF
4. Origin and transmission of Feline coronavirus type I in domestic cats from Northern Italy: a phylogeographic approach.
- Author
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Lauzi, Stefania, Stranieri, Angelica, Giordano, Alessia, Luzzago, Camilla, Zehender, Gianguglielmo, Paltrinieri, Saverio, and Ebranati, Erika
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CATS , *FELIDAE , *CORONAVIRUSES , *CAT diseases , *COMMUNICABLE diseases , *DEFINITIONS - Abstract
• FCoV type I only was observed in cats from Northern Italy. • A high genetic variability of the Italian FCoV circulating strains was observed. • Different strains of FCoV cocirculate in Northern Italy. • FCoV likely originated in USA in the 1950s-1970s and thereafter spread to Europe. • In the last decade FCoV segregates mainly on the basis of geographic origin. Feline coronavirus (FCoV) is responsible, along with an inadequate immune response of the host, for Feline infectious peritonitis (FIP), one of the most frequent and deadly infectious feline disease worldwide. This study analyzed the genetic characteristics of the spike (S) gene of 33 FCoVs circulating in Northern Italy between 2011 and 2015 in cats with or without FIP. In order to reconstruct the most probable places of origin and dispersion of FCoV among Italian cats, a phylogeographic approach was performed based on 106 FCoV S gene partial sequences from cats, including the 33 novel Italian sequences and 73 retrieved from public databases. Only FCoV type I was found in the Italian cats. The estimated mean evolutionary rate of FCoV was 2.4 × 10-2 subs/site/year (95% HPD: 1.3-3.7 × 10-2), confirming the high genetic variability in the circulating strains. All the isolates clustered in a unique highly significant clade that likely originated from USA between the 1950s and the 1970s, confirming the first descriptions of the disease in American cats. Our results suggest that from USA the virus likely entered Germany and thereafter spread to other European countries. Phylogeography showed that sequences segregated mainly by geographical origin. In the 2010s Italian sequences clustered in different subclades, confirming that different strains cocirculate in Italy. Further studies on archival samples and other genetic regions of FCoV are suggested in order to confirm the present results and to reconstruct a more in-depth detailed virus dispersion pattern for the definition of possible control measures. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
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