Search

A total of 325 Listeria monocytogenes isolates from human, food and environmental sources were ribotyped with the Qualicon Automated Riboprinter Microbial Characterization System, using the enzyme EcoR1. The human isolates (n=137) represented all isolates from clinical cases in Norway from 1992 to 2005. The food and environmental isolates (n=188) were collected from different food related sources in Norway in the period 1989-2002. A total of 37 ribotypes were differentiated. Most common ribotypes (i.e., ribotypes represented by >5 isolates) were isolated from human as well as food and environmental sources. The exceptions were ribotypes DUP-1062D and 1058A, which were found only among food and environmental samples (14 and 5 times, respectively), and DUP-1042A, which was identified only among human clinical isolates. DUP-1030A, the most frequent ribotype among the human isolates, as well as ribotypes DUP-1042B, DUP-1042C, DUP-1049B and DUP-1062B were identified from both foods, environmental and human sources. DUP-1039C (n=54) and DUP-1045B (n=27) were frequently isolated from patients, food and environmental samples. The isolates were classified into lineages based on ribotyping results. The lineage I strain DUP-1038B was isolated every year from 1992 to 2005 from the human clinical samples. Out of 137 listeriosis cases, 76 (55.6%) were caused by lineage II strains. We found a considerable overlap between ribotypes, lineages and isolation sources. It does not seem possible to establish food strain specific regulations for L. monocytogenes based on ribotyping. [ABSTRACT FROM AUTHOR]

Five salmon processing plants were investigated for periods of eight (plants A and B) and four (plants C, D and E) months for the occurrence of Listeria monocytogenes. A total of 226 strains of L. monocytogenes were isolated and subtyped. Automated ribotyping with DuPont Qualicon RiboPrinter® system with the enzyme EcoR1 was used to differentiate and characterize isolates for simple molecular tracking. The isolated strains could be divided into 16 DuPont ribotypes. The ribotypes DUP-1023C, DUP-1039C, DUP-1044E, DUP-1045B, DUP-1046A, DUP-1062B and 1062C were found to be persistent in the plants, as they were found through the whole sampling period. The remaining 9 ribotypes were isolated sporadically. Five of the persistent subtypes (DUP-1023C, DUP-1039C, DUP-1046A, DUP-1062B and 1062C) where isolated in high numbers. Ribotype DUP-1045B and DUP-1039C were found in four of the five investigated plants. [ABSTRACT FROM AUTHOR]