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8 results on '"Zhang, Baoshan"'

1. Enhancing Anti-SARS-CoV-2 Neutralizing Immunity by Genetic Delivery of Enveloped Virus-like Particles Displaying SARS-CoV-2 Spikes

Author

Yang, Yongping, primary, Kong, Wing-Pui, additional, Liu, Cuiping, additional, Ruckwardt, Tracy J., additional, Tsybovsky, Yaroslav, additional, Wang, Lingshu, additional, Wang, Shuishu, additional, Biner, Daniel W., additional, Chen, Man, additional, Liu, Tracy, additional, Merriam, Jonah, additional, Olia, Adam S., additional, Ou, Li, additional, Qiu, Qi, additional, Shi, Wei, additional, Stephens, Tyler, additional, Yang, Eun Sung, additional, Zhang, Baoshan, additional, Zhang, Yi, additional, Zhou, Qiong, additional, Rawi, Reda, additional, Koup, Richard A., additional, Mascola, John R., additional, and Kwong, Peter D., additional

Published
2023
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2. Extraordinary Titer and Broad Anti-SARS-CoV-2 Neutralization Induced by Stabilized RBD Nanoparticles from Strain BA.5.

Author

Wang, Zhantong, Zhang, Baoshan, Ou, Li, Qiu, Qi, Wang, Lingshu, Bylund, Tatsiana, Kong, Wing-Pui, Shi, Wei, Tsybovsky, Yaroslav, Wu, Lingyuan, Zhou, Qiong, Chaudhary, Ridhi, Choe, Misook, Dickey, Thayne H., El Anbari, Mohammed, Olia, Adam S., Rawi, Reda, Teng, I-Ting, Wang, Danyi, and Wang, Shuishu

Subjects
TITERS, NANOPARTICLES, COVID-19 vaccines, DETECTION limit, IMMUNE response
Abstract

The receptor-binding domain (RBD) of the SARS-CoV-2 spike is a primary target of neutralizing antibodies and a key component of licensed vaccines. Substantial mutations in RBD, however, enable current variants to escape immunogenicity generated by vaccination with the ancestral (WA1) strain. Here, we produce and assess self-assembling nanoparticles displaying RBDs from WA1 and BA.5 strains by using the SpyTag:SpyCatcher system for coupling. We observed both WA1- and BA.5-RBD nanoparticles to degrade substantially after a few days at 37 °C. Incorporation of nine RBD-stabilizing mutations, however, increased yield ~five-fold and stability such that more than 50% of either the WA1- or BA.5-RBD nanoparticle was retained after one week at 37 °C. Murine immunizations revealed that the stabilized RBD-nanoparticles induced ~100-fold higher autologous neutralization titers than the prefusion-stabilized (S2P) spike at a 2 μg dose. Even at a 25-fold lower dose where S2P-induced neutralization titers were below the detection limit, the stabilized BA.5-RBD nanoparticle induced homologous titers of 12,795 ID50 and heterologous titers against WA1 of 1767 ID50. Assessment against a panel of β-coronavirus variants revealed both the stabilized BA.5-RBD nanoparticle and the stabilized WA1-BA.5-(mosaic)-RBD nanoparticle to elicit much higher neutralization breadth than the stabilized WA1-RBD nanoparticle. The extraordinary titer and high neutralization breadth elicited by stabilized RBD nanoparticles from strain BA.5 make them strong candidates for next-generation COVID-19 vaccines. [ABSTRACT FROM AUTHOR]

Published
2024
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3. Assessment of Crosslinkers between Peptide Antigen and Carrier Protein for Fusion Peptide-Directed Vaccines against HIV-1

Author

Ou, Li, primary, Gulla, Krishana, additional, Biju, Andrea, additional, Biner, Daniel W., additional, Bylund, Tatsiana, additional, Changela, Anita, additional, Chen, Steven J., additional, Zheng, Cheng-Yan, additional, Cibelli, Nicole, additional, Corrigan, Angela R., additional, Duan, Hongying, additional, Gonelli, Christopher A., additional, Kong, Wing-Pui, additional, Cheng, Cheng, additional, O’Dell, Sijy, additional, Sarfo, Edward K., additional, Shaddeau, Andrew, additional, Wang, Shuishu, additional, Vinitsky, Alison, additional, Yang, Yanhong, additional, Zhang, Baoshan, additional, Zhang, Yaqiu, additional, Koup, Richard A., additional, Doria-Rose, Nicole A., additional, Gall, Jason G., additional, Mascola, John R., additional, and Kwong, Peter D., additional

Published
2022
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4. Design of Alphavirus Virus-Like Particles Presenting Circumsporozoite Junctional Epitopes That Elicit Protection against Malaria

Author

Francica, Joseph R., primary, Shi, Wei, additional, Chuang, Gwo-Yu, additional, Chen, Steven J., additional, Da Silva Pereira, Lais, additional, Farney, S. Katie, additional, Flynn, Barbara J., additional, Ou, Li, additional, Stephens, Tyler, additional, Tsybovsky, Yaroslav, additional, Wang, Lawrence T., additional, Anderson, Alexander, additional, Beck, Zoltan, additional, Dillon, Marlon, additional, Idris, Azza H., additional, Hurlburt, Nicholas, additional, Liu, Tracy, additional, Zhang, Baoshan, additional, Alving, Carl R., additional, Matyas, Gary R., additional, Pancera, Marie, additional, Mascola, John R., additional, Kwong, Peter D., additional, and Seder, Robert A., additional

Published
2021
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5. Single-Shot Vaccines against Bovine Respiratory Syncytial Virus (BRSV): Comparative Evaluation of Long-Term Protection after Immunization in the Presence of BRSV-Specific Maternal Antibodies

Author

Valarcher, Jean François, primary, Hägglund, Sara, additional, Näslund, Katarina, additional, Jouneau, Luc, additional, Malmström, Ester, additional, Boulesteix, Olivier, additional, Pinard, Anne, additional, Leguéré, Dany, additional, Deslis, Alain, additional, Gauthier, David, additional, Dubuquoy, Catherine, additional, Pietralunga, Vincent, additional, Rémot, Aude, additional, Falk, Alexander, additional, Shevchenko, Ganna, additional, Bergström Lind, Sara, additional, Von Brömssen, Claudia, additional, Vargmar, Karin, additional, Zhang, Baoshan, additional, Kwong, Peter D., additional, Rodriguez, María Jose, additional, Garcia Duran, Marga, additional, Schwartz-Cornil, Isabelle, additional, Taylor, Geraldine, additional, and Riffault, Sabine, additional

Published
2021
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6. Newcastle Disease Virus-Like Particles Displaying Prefusion-Stabilized SARS-CoV-2 Spikes Elicit Potent Neutralizing Responses

Author

Yang, Yongping, primary, Shi, Wei, additional, Abiona, Olubukola M., additional, Nazzari, Alexandra, additional, Olia, Adam S., additional, Ou, Li, additional, Phung, Emily, additional, Stephens, Tyler, additional, Tsybovsky, Yaroslav, additional, Verardi, Raffaello, additional, Wang, Shuishu, additional, Werner, Anne, additional, Yap, Christina, additional, Ambrozak, David, additional, Bylund, Tatsiana, additional, Liu, Tracy, additional, Nguyen, Richard, additional, Wang, Lingshu, additional, Zhang, Baoshan, additional, Zhou, Tongqing, additional, Chuang, Gwo-Yu, additional, Graham, Barney S., additional, Mascola, John R., additional, Corbett, Kizzmekia S., additional, and Kwong, Peter D., additional

Published
2021
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7. A Single Shot Pre-fusion-Stabilized Bovine RSV F Vaccine is Safe and Effective in Newborn Calves with Maternally Derived Antibodies

Author

Riffault, Sabine, primary, Hägglund, Sara, additional, Guzman, Efrain, additional, Näslund, Katarina, additional, Jouneau, Luc, additional, Dubuquoy, Catherine, additional, Pietralunga, Vincent, additional, Laubreton, Daphné, additional, Boulesteix, Olivier, additional, Gauthier, David, additional, Remot, Aude, additional, Boukaridi, Abdelhak, additional, Falk, Alexander, additional, Shevchenko, Ganna, additional, Lind, Sara, additional, Vargmar, Karin, additional, Zhang, Baoshan, additional, Kwong, Peter, additional, Rodriguez, María, additional, Duran, Marga, additional, Schwartz-Cornil, Isabelle, additional, Eléouët, Jean-François, additional, Taylor, Geraldine, additional, and Valarcher, Jean, additional

Published
2020
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8. Extraordinary Titer and Broad Anti-SARS-CoV-2 Neutralization Induced by Stabilized RBD Nanoparticles from Strain BA.5.

Author

Wang Z, Zhang B, Ou L, Qiu Q, Wang L, Bylund T, Kong WP, Shi W, Tsybovsky Y, Wu L, Zhou Q, Chaudhary R, Choe M, Dickey TH, El Anbari M, Olia AS, Rawi R, Teng IT, Wang D, Wang S, Tolia NH, Zhou T, and Kwong PD

Abstract

The receptor-binding domain (RBD) of the SARS-CoV-2 spike is a primary target of neutralizing antibodies and a key component of licensed vaccines. Substantial mutations in RBD, however, enable current variants to escape immunogenicity generated by vaccination with the ancestral (WA1) strain. Here, we produce and assess self-assembling nanoparticles displaying RBDs from WA1 and BA.5 strains by using the SpyTag:SpyCatcher system for coupling. We observed both WA1- and BA.5-RBD nanoparticles to degrade substantially after a few days at 37 °C. Incorporation of nine RBD-stabilizing mutations, however, increased yield ~five-fold and stability such that more than 50% of either the WA1- or BA.5-RBD nanoparticle was retained after one week at 37 °C. Murine immunizations revealed that the stabilized RBD-nanoparticles induced ~100-fold higher autologous neutralization titers than the prefusion-stabilized (S2P) spike at a 2 μg dose. Even at a 25-fold lower dose where S2P-induced neutralization titers were below the detection limit, the stabilized BA.5-RBD nanoparticle induced homologous titers of 12,795 ID 50 and heterologous titers against WA1 of 1767 ID 50 . Assessment against a panel of β-coronavirus variants revealed both the stabilized BA.5-RBD nanoparticle and the stabilized WA1-BA.5-(mosaic)-RBD nanoparticle to elicit much higher neutralization breadth than the stabilized WA1-RBD nanoparticle. The extraordinary titer and high neutralization breadth elicited by stabilized RBD nanoparticles from strain BA.5 make them strong candidates for next-generation COVID-19 vaccines.

Published
2023
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