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Start Over Author "Jose Benedito" Journal ultrasonics sonochemistry
5 results on '"Jose Benedito"'

1. High-power ultrasound pretreatment for enhanced protein extraction from lupin flour: Impact on yield, anti-technological and anti-nutritional factors, and techno-functional properties

Author

Paola Navarro-Vozmediano, Esperanza Dalmau, Jose Benedito, and Jose V. Garcia-Perez

Subjects
Ultrasound, Lupin protein, Pretreatment, Anti-nutritional factors, Anti-technological factors, Techno-functional properties, Chemistry, QD1-999, Acoustics. Sound, QC221-246
Abstract

Quality of lupin protein is considerably compromised by the presence of anti-nutritional (ANF), such as polyphenols, alkaloids and saponins and anti-technological factors (ATF), like polyphenols and fat. This work explores how high-power ultrasound-assisted (HPU) pretreatment of lupin flour (LF) before protein extraction could affect ANF and ATF content, as well as protein yield and techno-functional properties. LF pretreatments for 3 and 9 min, using water and ethanol–water (1:4 v/v), were carried using conventional mechanical stirring (952 rpm) and HPU (sonotrode, 24 kHz) at 30 and 60 °C. Ultrasonic field was characterized by computing acoustic pressure from frequency spectra analysis.In general terms, pretreatment reduced noticeably ANF and ATF in both LF and protein isolate (LPI), while also producing a protein concentrate (LPC, avg. 65 g protein/100 g) with low ANF and ATF content. Total protein yield, adding LPC and LPI, was increased by 15 % due to the pretreatment, which also enhanced the techno-functional properties of LPI, such as water absorption index (avg. 26 %) and foaming (avg. 8 %) and emulsifying properties (avg. 14 %). But, the efficiency of the pretreatment was largely affected by process variables. In particular, HPU reduced fat (avg. 27 %), saponin (avg. 21 %) and phenolic content (avg. 17 %), as well as antioxidant activity (avg. 11 %). In addition, the highest protein yield was achieved by sonication using water at 30 °C. This may be explained by ultrasonic field measurements, which revealed higher acoustic pressure levels under these conditions.

Published
2025
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2. Combination of supercritical CO2 and high-power ultrasound for the inactivation of fungal and bacterial spores in lipid emulsions

Author

Angela Gomez-Gomez, Edmundo Brito-de la Fuente, Críspulo Gallegos, Jose V. Garcia-Perez, and Jose Benedito

Subjects
Spores, Inactivation, Supercritical fluids, Ultrasound, Emulsions, Chemistry, QD1-999, Acoustics. Sound, QC221-246
Abstract

For the first time, this study addresses the intensification of supercritical carbon dioxide (SC-CO2) treatments using high-power ultrasound (HPU) for the inactivation of fungal (Aspergillus niger) and bacterial (Clostridium butyricum) spores in oil-in-water emulsions. The inactivation kinetics were analyzed at different pressures (100, 350 and 550 bar) and temperatures (50, 60, 70, 80, 85 °C), depending on the microorganism, and compared to the conventional thermal treatment. The inactivation kinetics were satisfactorily described using the Weibull model.Experimental results showed that SC-CO2 enhanced the inactivation level of both spores when compared to thermal treatments. Bacterial spores (C. butyricum) were found to be more resistant to SC-CO2 + HPU, than fungal (A. niger) ones, as also observed in the thermal and SC-CO2 treatments. The application of HPU intensified the SC-CO2 inactivation of C. butyricum spores, e.g. shortening the total inactivation time from 10 to 3 min at 85 °C. However, HPU did not affect the SC-CO2 inactivation of A. niger spores. The study into the effect of a combined SC-CO2 + HPU treatment has to be necessarily extended to other fungal and bacterial spores, and future studies should elucidate the impact of HPU application on the emulsion’s stability.

Published
2021
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3. Combination of supercritical CO2 and high-power ultrasound for the inactivation of fungal and bacterial spores in lipid emulsions

Author

Edmundo Brito-de la Fuente, Angela Gomez-Gomez, José V. García-Pérez, Críspulo Gallegos, and Jose Benedito

Subjects
Spores, Acoustics and Ultrasonics, TECNOLOGIA DE ALIMENTOS, Microorganism, QC221-246, 02 engineering and technology, 010402 general chemistry, 01 natural sciences, Endospore, Inactivation, Inorganic Chemistry, Ultrasound, Chemical Engineering (miscellaneous), Environmental Chemistry, Radiology, Nuclear Medicine and imaging, Food science, QD1-999, Clostridium butyricum, Supercritical fluids, Supercritical carbon dioxide, biology, Chemistry, Organic Chemistry, Aspergillus niger, fungi, Acoustics. Sound, 021001 nanoscience & nanotechnology, biology.organism_classification, Supercritical fluid, 0104 chemical sciences, Spore, Emulsion, Emulsions, 0210 nano-technology
Abstract

[EN] For the first time, this study addresses the intensification of supercritical carbon dioxide (SC-CO2) treatments using high-power ultrasound (HPU) for the inactivation of fungal (Aspergillus niger) and bacterial (Clostridium butyricum) spores in oil-in-water emulsions. The inactivation kinetics were analyzed at different pressures (100, 350 and 550 bar) and temperatures (50, 60, 70, 80, 85 °C), depending on the microorganism, and compared to the conventional thermal treatment. The inactivation kinetics were satisfactorily described using the Weibull model. Experimental results showed that SC-CO2 enhanced the inactivation level of both spores when compared to thermal treatments. Bacterial spores (C.butyricum) were found to be more resistant to SC-CO2 + HPU, than fungal (A.niger) ones, as also observed in the thermal and SC-CO2 treatments. The application of HPU intensified the SC-CO2 inactivation of C.butyricum spores, e.g. shortening the total inactivation time from 10 to 3 min at 85 °C. However, HPU did not affect the SC-CO2 inactivation of A.niger spores. The study into the effect of a combined SC-CO2 + HPU treatment has to be necessarily extended to other fungal and bacterial spores, and future studies should elucidate the impact of HPU application on the emulsion's stability, The authors acknowledge the financial support from Fresenius Kabi Deutschland GmbH, Germany

Published
2021

4. Combination of supercritical CO

Author

Angela, Gomez-Gomez, Edmundo, Brito-de la Fuente, Críspulo, Gallegos, Jose V, Garcia-Perez, and Jose, Benedito

Subjects
Spores, Bacterial, Spores, Supercritical fluids, Microbial Viability, fungi, Carbon Dioxide, Spores, Fungal, Lipids, Inactivation, Ultrasound hybridized technologies: A new breathing for Sonochemistry, Ultrasonic Waves, Ultrasound, Clostridium butyricum, Emulsions, Aspergillus niger
Abstract

Highlights • SC-CO2 at temperatures > 60 °C was effective to inactivate spores in lipid emulsions. • C. butyricum spores were more resistant to the SC-CO2 treatments than A. niger spores. • HPU intensified the inactivation of C. butyricum spores while no effect was found on A. niger. • Higher temperatures increase the SC-CO2 + HPU spore inactivation. • Pressure increase improved SC-CO2 + HPU spore inactivation up to 350 bar., For the first time, this study addresses the intensification of supercritical carbon dioxide (SC-CO2) treatments using high-power ultrasound (HPU) for the inactivation of fungal (Aspergillus niger) and bacterial (Clostridium butyricum) spores in oil-in-water emulsions. The inactivation kinetics were analyzed at different pressures (100, 350 and 550 bar) and temperatures (50, 60, 70, 80, 85 °C), depending on the microorganism, and compared to the conventional thermal treatment. The inactivation kinetics were satisfactorily described using the Weibull model. Experimental results showed that SC-CO2 enhanced the inactivation level of both spores when compared to thermal treatments. Bacterial spores (C. butyricum) were found to be more resistant to SC-CO2 + HPU, than fungal (A. niger) ones, as also observed in the thermal and SC-CO2 treatments. The application of HPU intensified the SC-CO2 inactivation of C. butyricum spores, e.g. shortening the total inactivation time from 10 to 3 min at 85 °C. However, HPU did not affect the SC-CO2 inactivation of A. niger spores. The study into the effect of a combined SC-CO2 + HPU treatment has to be necessarily extended to other fungal and bacterial spores, and future studies should elucidate the impact of HPU application on the emulsion’s stability.

Published
2020

5. Non-thermal pasteurization of lipid emulsions by combined supercritical carbon dioxide and high-power ultrasound treatment

Author

Edmundo Brito-de la Fuente, Angela Gomez-Gomez, José V. García-Pérez, Jose Benedito, and Críspulo Gallegos

Subjects
Materials science, Acoustics and Ultrasonics, Inactivation kinetics, Pasteurization, 02 engineering and technology, 010402 general chemistry, 01 natural sciences, law.invention, Inorganic Chemistry, law, Oil content, Thermal, Chemical Engineering (miscellaneous), Environmental Chemistry, Radiology, Nuclear Medicine and imaging, Supercritical carbon dioxide, business.industry, Organic Chemistry, Ultrasound, 021001 nanoscience & nanotechnology, 0104 chemical sciences, Process conditions, Chemical engineering, 0210 nano-technology, business, High power ultrasound
Abstract

Supercritical carbon dioxide (SC-CO2) is a novel method for food pasteurization, but there is still room for improvement in terms of the process shortening and its use in products with high oil content. This study addressed the effect of high power ultrasound (HPU) on the intensification of the SC-CO2 inactivation of E. coli and B. diminuta in soybean oil-in-water emulsions. Inactivation kinetics were obtained at different pressures (100 and 350 bar), temperatures (35 and 50 °C) and oil contents (0, 10, 20 and 30%) and were satisfactorily described using the Weibull model. The experimental results showed that for SC-CO2 treatments, the higher the pressure or the temperature, the higher the level of inactivation. Ultrasound greatly intensified the inactivation capacity of SC-CO2, shortening the process time by approximately 1 order of magnitude (from 50 to 90 min to 5–10 min depending on the microorganism and process conditions). Pressure and temperature also had a significant (p

Published
2020

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