1. Molecular analyses of Salmonella serotype Typhi and Salmonella serotype Paratyphi B strains isolated in Turkey.
- Author
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Dolapçi, İştar, Erdem, Birsel, Tekelı, Alper, Us, Ebru, Bayramova, Mehseti, Saran, Begüm, and Şahın, Fikret
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SALMONELLA , *SEROTYPES , *PLASMIDS , *POLYMERASE chain reaction , *ELECTROPHORESIS , *ENZYMES - Abstract
Aim: To investigate the characteristics of Salmonella serotype Typhi and Salmonella serotype Paratyphi B, which still remain significant in Turkey. Materials and methods: Eighteen S. Typhi and 42 S. Paratyphi B strains isolated from clinical samples were investigated by plasmid profile analysis, enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR), and pulsed field gel electrophoresis (PFGE) methods. Results: Plasmid profile analysis showed that 10 out of 18 (56%) S. Typhi isolates harbored 1 to 3 plasmids, and 11 out of 42 (26.2%) S. Paratyphi B isolates harbored 1 to 4 plasmids. PFGE performed with restriction enzyme AvrII grouped both S. Typhi and S. Paratyphi B isolates into 14 clusters; with restriction enzyme XbaI, S. Typhi and S. Paratyphi B isolates were grouped into 13 and 12 clusters, respectively. Conclusion: These results showed that plasmid profile analysis and PFGE are reliable and discriminative methods complementing the phenotypic characters of S. Typhi and S. Paratyphi B isolates, and may contribute to the epidemiologic investigation of typhoid or paratyphoid diseases. The study presented here is the first report on S. Typhi and S. Paratyphi B isolates from Turkey investigated by plasmid profile analysis, ERIC-PCR, and PFGE methods. [ABSTRACT FROM AUTHOR]
- Published
- 2010
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