1. Reappraisal of HLA Antibody Analysis and Crossmatching in Kidney Transplantation
- Author
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Chung-Jye Hung, Po-Chang Lee, Yih Jyh Lin, T C. Chou, J P. Chuang, and Shen-Shin Chang
- Subjects
Enzyme-Linked Immunosorbent Assay ,Human leukocyte antigen ,Antigen ,Antibody Specificity ,HLA Antigens ,Isoantibodies ,Preoperative Care ,medicine ,Humans ,Hla antibodies ,Elisa method ,False Negative Reactions ,Kidney transplantation ,Retrospective Studies ,HLA-D Antigens ,Transplantation ,biology ,business.industry ,Histocompatibility Testing ,Histocompatibility Antigens Class I ,Follow up studies ,medicine.disease ,Kidney Transplantation ,Disease control ,Immunology ,biology.protein ,Surgery ,Antibody ,business ,Follow-Up Studies - Abstract
Enzyme-linked immunosorbent assay (ELISA) and flow cytometric techniques have been introduced to overcome the limited sensitivity and specificity of the CDC assay. This retrospective study used lambda antigen tray-mixed screening and Luminex HLA class I and II specificity assays to re-examine: (1) the accuracy with which detection of HLA antibody and specificity by ELISA predicts pretransplantation National Institutes of Health (NIH)/Centers for Disease Control and Prevention (CDC) crossmatch; and (2) a comparison of Luminex and ELISA methods to detect HLA antibodies. Sera from 481 patients awaiting kidney transplantation were tested using the ELISA method lambda antigen tray-mixed and using NIH-CDC to determine how well HLA antibodies detected using ELISA predicted crossmatches using CDC. Pretransplantation sera from 48 patients with follow-up data were retested using both ELISA lambda antigen tray-mixed and Luminex to compare the efficacy of the 2 methods.
- Published
- 2009