Objectives The aim of this study is to investigate the outcome of laparoscopic donor nephrectomy with vascular anomalies of the left renal vein. Patients and Methods Between August 2010 and September 2018, a total of 120 laparoscopic donor nephrectomies were performed at Kagoshima University. Among them, we experienced 7 cases of donors with anomalous left renal vein (circumaortic left renal vein n = 5, retroaortic left renal vein n = 1, left renal vein drainage into hemiazygos vein n = 1). We analyzed the following clinical outcomes: pneumoperitoneum time, estimated blood loss, warm ischemia time, length of hospital stay, and serum creatinine level of 1 month after surgery for evaluating the safety of laparoscopic donor nephrectomy. Results Among the 7 cases, 3 cases underwent transperitoneal approach, and 4 cases underwent retroperitoneal approach. The median pneumoperitoneum time was 168 (108–191) minutes. The median estimated blood loss was 90 (23–170) mL, and no donor required a blood transfusion. Median warm ischemia time was 4 (3–7) minutes. Length of hospital stay was 7 (6–9) days, and no readmission occurred. Median serum creatinine level of 1 month after surgery was 1.19 (0.84–1.74) mg/dL. Kidneys were transplanted successfully, and none of recipients required dialysis. Conclusions. Laparoscopic donor nephrectomy was safe for donors with an anomalous left renal vein. Preoperative recognition of anomalous left renal vein in computed tomography is important for avoiding hemorrhagic complication.