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ALTHOUGH the success of the first cold storage solution was attributed to its intracellular composition with low sodium and high potassium,1,2 the importance of impermeant agents has been shown and the potential deleterious effects of high potassium solutions have been suggested.3–5

ISCHEMIA-reperfusion injury after organ transplantation is a major cause of delayed graft function. The pathogenesis of ischemia-reperfusion injury are well correlated with alterations in mitochondrial function, namely, decrease in ATP synthesis, NAD(P)H level, and mitochondrial membrane potential and generation of mitochondrial permeability transition. A previous study has demonstrated that the pretreatment with trimetazidine (TMZ) prevented these ischemia-reperfusion deleterious effects at both the cellular and mitochondrial level. The aim of this study was to assess the effect of TMZ added to University of Wisconsin Solution (UW) during cold preservation in an isolated perfused pig kidney model against lipid peroxidation and renal medulla damage. After cold preservation (CP), kidneys were perfused as previously described.

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PRIMARY dysfunction or nonfunction of liver graft is still an important problem in liver transplantation despite surgical progress. The determination of new markers of graft viability and metabolic activities seems to be an important challenge to assess the early graft function. Proton nuclear magnetic resonance spectroscopy (NMRp) has previously demonstrated its interest in the evaluation of renal allograft dysfunction after ischemia reperfusion in isolated perfused pig kidney. For the kidney, NMRp study determines specific markers of function like TMAO and citrate. The aim of this study was to assess liver graft function in an isolated perfused rat liver model after 24 hours of preservation in Euro-Collins (EC) or University of Wisconsin (UW) solution.

DAMAGE sustained by the allograft during the process of transplantation has been shown to be influenced by cold ischemia-reperfusion injury, which is one of the principal “antigen-independent” factors. A previous study demonstrated that tissue injury occurs within isografts as compared to allografts, in the absence of alloantigen stimulation. In a rat model, a recent report suggests that molecules involved in T-cell costimulation may play a key role in ischemia-reperfusion in the kidney, independent of any alloimmune stimuli. The aim of this study was to assess the effect of the length of cold ischemic time in an autotransplant pig kidney model on delayed graft function and inflammatory cell infiltration.

PRIMARY and early allograft dysfunction remains a challenge for the nephrologist. Usually, the assessment of renal graft dysfunction following transplantation is based on nonspecific measurement of renal function or an invasive method such as transplant biopsy. A rapid diagnosis is necessary because a delay in treating early rejection deteriorates the prognosis of the allograft function. In addition, the antirejection treatment based on a mere clinical diagnosis of rejection could improve delayed graft function caused by acute tubular necrosis or cyclosporine damage through possible side effects of antirejection treatment. In the present study, we wanted to assess whether proton nuclear magnetic resonance (HNMR) spectroscopy can detect ischemic damage and predict acute tubular necrosis after pig kidney preservation in two preservation solutions (Euro-Collins: EC and University of Wisconsin: UW).