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Start Over Author "Sinclair, Caroline" Journal transgenic research
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1. Rapid conversion of EUCOMM/KOMP-CSD alleles in mouse embryos using a cell-permeable Cre recombinase.

Author

Ryder, Edward, Doe, Brendan, Gleeson, Diane, Houghton, Richard, Dalvi, Priya, Grau, Evelyn, Habib, Bishoy, Miklejewska, Evelina, Newman, Stuart, Sethi, Debarati, Sinclair, Caroline, Vyas, Sapna, Wardle-Jones, Hannah, Bottomley, Joanna, Bussell, James, Galli, Antonella, Salisbury, Jennifer, and Ramirez-Solis, Ramiro

Abstract

We describe here use of a cell-permeable Cre to efficiently convert the EUCOMM/KOMP-CSD tm1a allele to the tm1b form in preimplantation mouse embryos in a high-throughput manner, consistent with the requirements of the International Mouse Phenotyping Consortium-affiliated NIH KOMP2 project. This method results in rapid allele conversion and minimizes the use of experimental animals when compared to conventional Cre transgenic mouse breeding, resulting in a significant reduction in costs and time with increased welfare benefits. [ABSTRACT FROM AUTHOR]

Published
2014
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