1. Production of biologically active human granulocyte colony stimulating factor in the milk of transgenic goat
- Author
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J H, Ko, C S, Lee, K H, Kim, M G, Pang, J S, Koo, N, Fang, D B, Koo, K B, Oh, W S, Youn, G D, Zheng, J S, Park, S J, Kim, Y M, Han, I Y, Choi, J, Lim, S T, Shin, S W, Jin, K K, Lee, and O J, Yoo
- Subjects
Goats ,Recombinant Fusion Proteins ,Blotting, Western ,Genetic Vectors ,Caseins ,Enzyme-Linked Immunosorbent Assay ,Polymerase Chain Reaction ,Animals, Genetically Modified ,Mice, Inbred C57BL ,Mice ,Milk ,Granulocyte Colony-Stimulating Factor ,Animals ,Humans ,Female ,Cyclophosphamide ,Cell Division ,DNA Primers - Abstract
We have developed a transgenic female goat harboring goat beta-casein promoter/human granulocyte colony stimulating factor (G-CSF) fusion gene by microinjection into fertilized one-cell goat zygotes. Human G-CSF was produced at levels of up to 50 microg/ml in transgenic goat milk. Its biological activity was equivalent to recombinant human G-CSF expressed from Chinese hamster ovary (CHO) cell when assayed using in vitro HL-60 cell proliferation. Human G-CSF from transgenic goat milk increased the total number of white blood cells in C57BL/6N mice with leucopenia induced by cyclophosphamide (CPA). The secreted human G-CSF was glycosylated although the degree of O-glycosylation was lower compared to CHO cell-derived human G-CSF.
- Published
- 2000