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In rencent years, wine grape planting area expands rapidly in Xinjiang, but the wine grape still harvested by man. Fruit-pedicle separation is a key link in the mechanized harvesting , further a wine grape vibration separation experiment device of 4P -12 type double support was designed to study the operation mechanism of double support wine grape separation device based on vibration, and the device was manufactured by Shihezi Development Zone Shida Ruituo mechanical equipment Co. ltd. The Double Support Wine Grape Separation Experiment Device based on Vibration consisted of driving parts, RSSR mechanism, and working parts, flat double rocker mechanism. The rotation of driving shaft was switched to the rotation of axle vibration rocker shaft by RSSR mechanism, and the rotation of axle vibration rocker shaft was switched to the swing of rib componet. When the vibration device was working, the driving shaft of driving part was drived by motor. The motion drived the eccentric sleeve and was transfered to the axle vibration rock by adjusting linkage, spherical plain rod ends bearing and vibration rocker, and then was transferred to axle driven rocker through active rocker and connecting rod to make the grapevine vibrate, and the grape was separated by the force of inertia. Equivalent plane institutions act and the complex vector method were used to analyze the theories of RSSR mechanism and flat double rocker mechanism, and the models of kinematics and dynamics of working parts were established. At the same time, the main influence factors of separation, motor speed, position and vibration rocker length were determined through the analysis of the models. The Cabernet Sauvignon were selected as samples planted in Xinjiang infantry regiment of the eighth division (Shihezi city) 152 group. The level of each factor was determined by single factor experiment, and L9(34) orthogonal test plan was designed. The wine grape branches with good growth, fruiting of uniform, 0.8~1 m length, were pruned as sample which were refrigerated rapidly to retain freshness. An experiment was conducted according to the orthogonal experiment table and initial mass. Mass of after-separation and Branch mass were recorded to calculate separation rate with the foumula. Visual and variance analysis methods were uesd to analyze the data. The results of visual analysis method indicated that the separation rate of optimal scheme was that the motor speed was 750 r/min, vibration position was 550 mm and vibration rocker length was 125 mm. Analysis of variance showed that motor speed and position influenced separation effect significantly, while the vibration rocker length was affected insignificantly. In addition, the device was simulated in Solidworks and experimented under the high speed-camera after adjusting to the optimal of the optimal scheme, and the working parts were analysised by the high speed-camera Analysis Software. The optimal vibration curves of separation angular velocity and angular acceleration were achieved by Solidworks simulation and high-speed camera, and were fitting each other well, and the correctness of the device's operation mechanism was verified. The study could provide a reference to the design and optimization of wine grape harvester. [ABSTRACT FROM AUTHOR]