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Banana fusarium wilt has a significant adverse effect on the banana industry. It is worth noting that fusarium oxysporum f.sp. cubense 4(Foc4) is one of the most devastating and widespread pathogens of banana fusarium wilt in the world. It is a catastrophic threat to the global banana industry. At present, there is still no technology that can cure bananas infected with banana fusarium wilt. Therefore, it is extremely important for banana planting industry to detect banana fusarium wilt in time and do a good job in prevention and control. In order to realize the early accurate detection of banana fusarium wilt race 4, this study proposed a detection method of double probe paper-based sensor based on colloidal gold, which increased the use of signal enhancement probes to increase the binding amount of colloidal gold, thereby reducing the detection limit. Two kinds of colloidal gold particles with different particle sizes were combined with the corresponding detection probes and signal enhancement probes to form a double gold label probe system. The large particle size colloidal gold was used with the detection probe, while the small particle size colloidal gold was paired with the signal enhancement probe. The sample being tested was mixed with this double gold-labeled probe system. The Foc4 target sequence in the sample would be paired with the double gold-labeled probe to form a ' gold-labeled probe-target sequence-T-line probe ' complex, which continued to migrate to the water-absorbing pad. The complex was captured on the test area of the sensor. Within 10 minutes, the test paper would generate a visible target product that could be observed by the naked eye at both the test line and the quality control line areas, thus achieving qualitative detection. For quantitative detection of Foc4, the light intensity of the test strip would be further analyzed using software. The light intensity peak area was obtained and substituted into the standard curve to obtain the concentration of Foc4 detected and realize quantitative detection. Experimental results demonstrate that the detection limit of the dual-probe paper-based sensor is 0.001 nmol/L, which is 100 times of that of the traditional paper-based sensor, and the detection sensitivity is improved. The sensitivity test results demonstrated a strong linear relationship between the concentration of Foc4 and the peak area of the test line light intensity within the concentration range of 0.001-1 000.000 nmol/L. This finding suggests that the sensor can be effectively employed for quantitative detection. To assess specificity, a high concentration of non-complementary probe was utilized as the target sequence for detection and compared with the detection results of samples containing Foc4. The experiment revealed the absence of a red band at the test line when the test paper was detected by the noncomplementary sequence. Importantly, this observation indicated that the presence of the non-complementary sequence had minimal impact on the detection effect, thus affirming the sensor's excellent specificity. Finally, the paper-based sensor was used to detect Foc4 in banana leaves. The average recovery rate was 77.6%-102.3%, and the relative standard deviation was 7.4%-7.7%, indicating that it can be used for on-site detection of Foc4. The detection technology possesses the characteristics of being low-cost and easy to operate. In comparison to traditional detection methods, it enables timely, rapid and accurately determination of the presence of Foc4. Compared with the existing molecular detection technology, the detection can be completed without the need of expensive equipment or specialized personnel. This feature makes it highly suitable for widespread use. The dual-probe paper-based sensor realizes the combination of nucleic acid lateral chromatography detection technology and banana fusarium wilt detection. Its potential for widespread adoption in the early disease warning system of the banana industry is substantial. Furthermore, this technology can be combined with traditional polymerase chain reaction (PCR) and other techniques to facilitate on-site detection of Foc4. [ABSTRACT FROM AUTHOR]

A high-precision mechanized seeding can greatly contribute to the vegetable production at present. In this study, a spotting glue-paper tape precision seeder was designed for the small seed vegetables, particularly combining the seed rope and printing seeding. Three components were also divided in the seeder, including the peristaltic pump glue, ratchet spotting glue, and seed metering device. Among them, the glue was a mixture of pregelatinized starch and water. Specifically, the glue volume and viscosity were the main factors affecting the number of adhered seeds. The glue point volume depended mainly on the rotation angle of the peristaltic pump during the movement. A pretest of seed adhesion was carried out to preliminarily determine the mass ratio of pregelatinized starch to water (less than 1:5), representing the glue viscosity. Furthermore, the frequency of pump and dispensing were analyzed to clarify the relationship between the peristaltic pump and ratchet speed, where the number of ratchet teeth was determined to be 4. As such, the matching was realized between the peristaltic pump and ratchet spotting glue. The ratchet profile was also designed, according to the spotting glue motion. A theoretical analysis was made on the movement of seeds in the seed guiding tube. It was found that the aggregation effect of seeds on the paper tape relied on the length and cross-sectional angle of the seed guiding tube, as well as the rotation speed of the socket wheel. The seed guiding tube was then optimized for the optimal structural parameters. A Box_Behnken test was also carried out, where the length and cross-sectional angle of the seed guiding tube, while the rotation speed of the socket wheel was taken as the test factors, whereas, the percentage of seeds in the effective area on the paper tape was taken as the test index. The results showed that the percentage of seeds in the effective area on the paper tape was 57.23%, when the length of the seed guiding tube was 56 mm, the section angle was 35°, and the rotation speed of the seed metering wheel was 30 r/min. An adhesion test was also performed on the Shanghai Qing 605 seeds with a diameter of 1.5-2.0 mm. The test factors were then selected as the roller angle of the peristaltic pump and the mass ratio of pregelatinized starch to water. An optimal combination of parameters was achieved, including the size of the glue point, and the mass ratio of pregelatinized starch to water. Once the roller rotation angle was 10° and the mass ratio of pre-gelatinized starch to water was 1:6, the single seed rate of glue point adhesion to seeds reached 97.4%. The adaptability test was also carried out on the whole machine on the working performance of different varieties of small seed vegetables. It was found that the single seed rates of Shanghai Qing 605 and Siji Chinese cabbage seeds were greater than 95% under the working speed of 0.5-1.5 m/s, whereas, the coefficient of variation of plant spacing was less than 10%, fully meeting the requirements of precision sowing indicators that specified in the standard NY/T1143-2006. Therefore, the single seed rate and the working efficiency of the improved seeder increased by 3.53%, and 50% than before. [ABSTRACT FROM AUTHOR]

A photoelectric detection method based on paper-based microfluidic chip in pesticide residues is proposed because the existing equipment has the problems of high cost, low degree of automation and large amount of reagent consumables. We design a kind of simple, portable, inexpensive paper-based microfluidic chip, whose bridge composite structure can enhance the uniformity of the color reaction of microfluidic enzyme, and also design the light path and circuit structure suitable for optical absorption reflection detection. The signal amplifying circuit and filter circuit are built to eliminate the interference of clutter and noise in photoelectric detection. Finally, a portable detection system of pesticide residue is established, which is an integrated test platform of the chemical reaction, the reflection effect in light absorption and the control of environment parameters. We observe and optimize the light source, chip placement angle, temperature and other process parameters of test platform in Jiangsu University in 2016. The reflection absorption effect of red LEDs (light-emitting diodes) is tested to be the best among the LEDs with different colors through detecting the paper-based chip color area. The response effect of the system is better when the angle is 10 according to the chip placement angle and reflected voltage data of photoelectric detection during the reaction. It is concluded that light absorption is the most under 35 ℃ and the chemical reaction is the most sufficient via comparing the color effect of the biochemical reaction of enzyme inhibition under different temperatures. We design different concentrations of pesticides, which are used for biochemical reactions of enzyme inhibition in different paper-based microfluidic chips. The resulting product displays blue color region in the paper-based chip, which can be characterized by the color of the different concentrations of pesticides. We use the photoelectric detection device to detect the color zone of the chip; the reflected voltage data can be obtained with this method, which correspond to different concentrations. Based on the experimental data, the model is established by the reflected voltage and the concentration of pesticide. The pesticide residues of test samples can be analyzed quantitatively based on the detection model. It can be concluded from the experimental results that with the method of photoelectric detection, the minimum detection limit of pesticide residues (trichlorfon and parathion) can reach 0.05 mg/L, meeting the national standard, and the resolution can reach 0.002 mg/L, much higher than that with the traditional method. Within the concentration range of pesticide residues, compared with the pesticide residues detector and the rapid detection card, the detection limit for the photoelectric detection is equivalent, but the consumable items are respectively 5.21% and 41.7% of the former 2 methods, and the reduction of time is respectively 23% and 33%. We propose a kind of pesticide residues detection system, which combines the photoelectric detection system and the paper-based microfluidic chip, and possesses the advantages of easy integration and low consumption. The research provides the theoretical basis and realistic foundation for making the pesticide residues detection develop to be portable and universal. [ABSTRACT FROM AUTHOR]

Soil water characteristic curve (SWCC) can show the soil water holding capacity, and is the relationship between the soil matric potential and saturation. The expansive soil has the characteristics of strong swell-shrink, developing fissures and over consolidation, especially under drying-wetting cycles. Its water holding capacity, which has an important impact on the soil properties, is totally different from the capacity of full soil. Therefore, how to exactly obtain the matric suction of expansive soil under drying-wetting cycles is very important to analyze the characteristics of mechanics and deformation of expansive soil. In order to analyze the change rule of matric suction of expansive soil under drying-wetting cycles, the artificial rainfall and evaporation was applied in drying-wetting tests of expansive soil. The specimens sized at 102 mm × 40 mm (diameter × height) were made by compaction apparatus and the initial dry density was 1.68 g/cm3. All the specimens were saturated after compaction and undergoing drying-wetting cycles in constant temperature (22 ℃) environment. The small electric fans were adopted to increase the rate of evaporation until the mass of specimens was kept constant under the condition of drying. Then the water vapours were sprayed to the surface of specimens by humidifiers until the process of infiltration stopped under the condition of wetting. That is one drying-wetting cycle so far. In this paper, 4 set of experiments under drying-wetting cycles were carried out and the cycle times were 1, 2, 3 and 4 respectively. Then 11 specimens sampled by cutting rings (61.8 mm × 20 mm (diameter × height)) in different volumetric water content were obtained in each process of drying and wetting respectively. The matric suction of each specimen was measured with filter paper of Whatman No. 42 and then the SWCCs were obtained under drying-wetting cycles. The corresponding air-entry values and residual values in different drying-wetting cycle times were calculated from SWCC. Ulteriorly, the different SWCCs obtained in different drying-wetting cycle times were fitted by the Fredlund SWCC model. Based on the above analysis, an SWCC model of expansive soil considering drying-wetting cycle effect was established finally. The results showed that: 1) With the increase of drying-wetting cycle time, the air-entry value of expansive soil was evidently reduced. Compared with the air-entry value (134.5 kPa) of expansive soil undergoing one drying-wetting cycle, the air-entry value was 58.5 kPa and reduced by 56.5% undergoing 4 drying-wetting cycles. The most effect of drying-wetting cycles on air-entry value occurred in the first cycle. 2) With the increase of drying-wetting cycle time, the residual value of expansive soil was also reduced. Compared with the residual value (1040.5 kPa) of expansive soil undergoing one drying-wetting cycle, the residual value was 528.5 kPa and reduced by 49.2% undergoing 4 drying-wetting cycles. Moreover, the residual value is reduced evidently after undergoing one drying-wetting cycle and it cannot yet be stabilized with the increase of drying-wetting cycle time. That means the effect of drying-wetting cycles on ari-entry value is much greater than on residual value. 3) The fitted parameters in the SWCC model of expansive soil considering the effect of drying-wetting cycle have a good linear correlation with the drying-wetting cycle time. With the increase of drying-wetting cycle time, the soil air-entry value and change rate of water content reduce while the residual value increases gradually. The results can provide a reference for the further analysis on soil matric suction and its application in engineering. [ABSTRACT FROM AUTHOR]

Most of the articles about the degradation performance of biodegradable nursery pots were focused on degradation morphology, strength of pot body, changes of soil, effect of raising seedings and so on. However, it rarely study on degradation rate, molecular structure of degradation process and changes of crystallinity. In order to study the degradation performance of biodegradable nursery pots, three types containing paper pot, undecomposed straw pot and decomposed straw pot were used to carry out the experiment in this paper. The degradation rate, molecular structure and crystallinity were evaluated and analyzed through the experiments of degradation test in soil, Fourier infrared scanning and x-ray diffraction. It is necessary to have a further research on degradation performance of biodegradable nursery containers and mechanism of degradation. The results showed that the degradation rate of paper pot with the best degradation property was growing at a rate of about 2 times. The maximum degradation rate was up to 85%. The undecomposed straw pot has always maintained a stable and increasing degradation rate. The undecomposed straw pot with degradation rate of 78% in the eighth week was higher than that of decomposed straw pot. The changes of mass loss and degradation rate were likely to be affect by a variety of microbial community structure. On the one hand, due to partial degradation of cellulose and semicellulose, the remaining part kept stable performance. On the other hand, the changes of microbial structure and activity affected the degradation performance. By comparison, FTIR absorption strength of the biodegradable nursery pots was decreased in different degree due to the changes of molecular structure during biodegradation. The absorption strength of paper pot decreased in the location of 3340 cm-1 and 2920 cm-1 indicated that the degradation of cellulose and hemicellulose. In addition, the relative peak of 1640 cm-1 and 1460 cm-1 decreased with the further degradation, showing that lignin was degraded in the biological reaction. The peak strength of CH stretch band was enhanced obviously at 892 cm-1. It showed that the substitution reaction of aromatic ring was an important part of the degradation process of lignin. The main group structure of undecomposed straw pot and decomposed straw pot were basically the same. The absorption peaks strength decreased at 3340 cm-1 and 1010 cm-1 might be due to the oxidative decomposition of benzene methanol unit. The peak strength of CH stretch band decreased at 2920 cm-1 probably because of the decomposition of carbohydrates and the fracture of partial fatty chain. The degradation of lignin resulted in the decrease of peak strength of 1650 cm-1. The x-ray diffraction peak intensity of paper pot with the best degradation property decreased obviously after 8 weeks test. The diffraction peak disappeared at 2 θ=15.6°. At the same time, The intensity of diffraction peak decreased at 2 θ=22.5°. In addition, it appeared new diffraction peaks at 2 θ=27° of undecomposed straw pot and decomposed straw pot. This might be due to the changes of crystalline which caused by the degradation of straw. The biodegradable nursery containers with excellent degradation property could improve the problem of environmental pollution effectively. [ABSTRACT FROM AUTHOR]

This study aims to improve the utilization rate of abandoned corn bracts, while decreasing the production cost of the bag of fruit cultivating in sustainable agriculture. Five representative bags of fruit cultivating were collected in the market as the controlled samples, including the plastic film bag A, special bag B for grape, one-, two- and three-layered bag C, D, and E. The basic structural characteristics of controlled samples were firstly analyzed. Then, alkali cooking was used to degum the corn bracts for the extraction of fibers. Subsequently, the corn bract fibers were cut into (5±0.5) mm long, thereby mixing evenly with the waste paper scraps in a ratio of 1.5:1. The wet mesh forming was adopted to prepare the fruit-cultivating bag material F. The mechanical properties of material F were tested after fabrication. The experimental results showed that the prepared material F behaved a strong tensile resistance, where the tensile loads for the dry and wet material F were up to 20.02 N and 13.79 N, respectively. Moreover, the retention rate reached 68.9% for the tensile load of wet material F. Furthermore, an excellent extension performance was achieved for the high requirements of mechanical properties in the fruit bagging. A scanning electronic microscopy (SEM) was adopted to observe the microstructure of the prepared materials F and the controlled samples in the market, further to analyze the distribution of holes in the matrix. The bagging properties were also measured, including air permeability, heat-insulating property, shading property, and water management ability. The experimental data demonstrated that the fruit-cultivating bag material F presented an excellent air permeability. Specifically, the air-flow rate and permeability of material were 73.5 mm/s and 173.7 L/m2 •s, respectively. In contrast to the controlled samples, the maximum air-flow rate and permeability were only 2.5 mm/s and 0.3 L/m2 •s, respectively. In addition, the heat loss of boiling water was about 265 kJ, when the prepared material was used to keep the boiling water for 60 min. The transmittance of the material F was about 0.4 % in the visible light range, and 0.09 % in the ultraviolet light range. The shielding properties showed that the prepared material ensured the photosynthesis of fruits during the growth process. Meanwhile, material F was expected to protect the fruit from ultraviolet light burning. Excellent ability of water conductivity was also achieved, where the core suction height reached 31 mm within 30 min. As such, the water in the fruit-cultivating bag during rainy days discharged quickly to enhance the quality of fruit products. Low wet evaporation indicated that the water retention performance was obviously better than that of the special bag B for the grape in the market. Most significantly, the thermal insulation performance of the new fabricated material was better than that of the controlled samples in the market. [ABSTRACT FROM AUTHOR]

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Copyright of Transactions of the Chinese Society of Agricultural Engineering is the property of Chinese Society of Agricultural Engineering and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

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Copyright of Transactions of the Chinese Society of Agricultural Engineering is the property of Chinese Society of Agricultural Engineering and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Transactions of the Chinese Society of Agricultural Engineering is the property of Chinese Society of Agricultural Engineering and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Transactions of the Chinese Society of Agricultural Engineering is the property of Chinese Society of Agricultural Engineering and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Heat exchangers serve as pivotal components in heat exchange systems. Compared with the straight channels, spiral channels have better performance due to the curvature of the centrifugal force generated by the secondary flow. Consequently, heat exchangers employing spiral channels find widespread application in agricultural engineering, including greenhouse air conditioning, water circulation systems, and agricultural product processing and storage for effective heat management. In this paper, we propose enhancing the heat transfer efficiency of spiral channels by introducing corrugations with longitudinal fins on the outer channel wall. To elucidate the impact of corrugation, we establish three-dimensional models of smooth spiral channels, corrugated spiral channels, and corrugated spiral channels with longitudinal fins. Initially, we investigate the influence of corrugated number on flow and heat transfer performance numerically. Through comparative analysis involving the development and variation of velocity and vorticity fields, average Nusselt number Nu, fanning friction factor f, average Dean number Dnm, secondary flow intensity Se under different corrugated numbers, and the Performance Evaluation factor of heat exchanger based on Comprehensive (PEC0), insights are drawn. Subsequently, longitudinal fins are integrated into the corrugated spiral channel, and a numerical study is conducted to assess the effect of varying geometric parameters of the longitudinal fin on the fluid flow and heat transfer performance inside the channel. Analysis of different longitudinal fin widths and heights on velocity and temperature fields, Nu, f, and Performance Evaluation factor of heat exchanger based on Comprehensive (PECw) of the channel is performed. The findings indicate that corrugated spiral channels outperform smooth spiral channels in heat transfer and flow performance. With increasing corrugated number n, secondary flow intensity within the corrugated spiral channel intensifies. Notably, at a Reynolds number of 550, a multi-vortex structure emerges in the corrugated spiral channel with a corrugated number of 21. At Reynolds number 750 and corrugated number 21, the Nusselt number of corrugated spiral channel increases by 47.08% compared to the smooth spiral channel. At the same time, the resistance loss in the channel also increases, and the Fanning friction factor of the corrugated spiral channel increases by 59.20%. When the Reynolds number is 750, the PEC0 of the corrugated spiral channel with 18 corrugations is the highest, which increases by 27.66% compared to the smooth spiral channel. On this basis, longitudinal fins are added to the corrugated spiral channel, and it is observed by numerical simulation that symmetrical longitudinal vortices are induced by the fins. Further, the longitudinal fins induce symmetrical longitudinal vortices, enhancing flow and heat transfer in the channel's middle section. Under the condition of constant fin width and increasing fin height, the comprehensive heat transfer performance of the channel has a maximum value, that is, when fin width w=1/3W, fin height h=1/6H and Reynolds number Re=250, the highest PECw is 1.157. When the fin height is fixed, increasing the fin width w will also make the PECw of the channel first increase and then decrease. The correlation formulas for Nusselt number Nu, Fanning friction factor f and secondary flow intensity Se in corrugated spiral channel are fitted, with deviations within ±14.0%, ±10.2% and ±4.4%, respectively. This provides a certain reference for the application of corrugated spiral channels with longitudinal fins in the thermal design and usage of heat exchangers. [ABSTRACT FROM AUTHOR]

Copyright of Transactions of the Chinese Society of Agricultural Engineering is the property of Chinese Society of Agricultural Engineering and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Transactions of the Chinese Society of Agricultural Engineering is the property of Chinese Society of Agricultural Engineering and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Transactions of the Chinese Society of Agricultural Engineering is the property of Chinese Society of Agricultural Engineering and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Transactions of the Chinese Society of Agricultural Engineering is the property of Chinese Society of Agricultural Engineering and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Transactions of the Chinese Society of Agricultural Engineering is the property of Chinese Society of Agricultural Engineering and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)