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To solve the environmental pollution and harm to human health caused by the use of adhesives in traditional paperboard, the development of environmentally friendly mycelium paperboard has become a hot topic. Due to the high dipole moment and hydrogen bonding between macromolecules, hyphae can form clamp shaped connection structures for nutrient reproduction and transfer, and can grow on natural agricultural waste, forming a natural biocomposite material. This study determined the combination of Ganoderma weberianum/corn straw, and designed single factor cultivation conditions such as cultivation time, straw particle size, and fructose addition to explore the effects on the mechanical properties, waterproof properties, and thermal decomposition performance of the hyphal paperboard formed by hot pressing. The optimal cultivation process was obtained through orthogonal experiments. The experimental results indicate that various factors have varying degrees of influence on the edgewise strength, flexural stiffness, and static bending strength and waterproofness of paperboard. Firstly, when the cultivation time was 30 days, the flexural stiffness reached 16.63 mN/m, which was 21.2% higher than the flexural stiffness of kraft paper (13.10 mN/m). Moreover, with the increase of cultivation time, the water absorption (24 hours) had also decreased from 3.92% to 1.27%. Secondly, previous research results have shown that the addition of fructose, glucose, and sucrose increases the biomass growth rate of strains. In this study, fructose was chosen as an exogenous nutrient, and the results showed that when a mass fraction of 5% fructose was added, the mechanical properties were the best, with a flexural stiffness of 18.87 mN/m, which was 30.5% higher than that of kraft paper (13.10 mN/m). Thirdly, the particle size of the sbstrate has a significant impact on the appearance and properties of the material. The results show that, from the appearance perspective, the mycelium paperboard made of 0.165～0.250 mm particle size has the closest appearance and feel to kraft paper. At the same time, during the testing period, it was found that the mycelium paperboard made of 0.165～0.250 mm straw was the least prone to deformation and loosening, and the molding effect was significantly improved compared to other components. In terms of mechanical properties, the edgewise strength increased by 17.4%, the static bending strength increased by 14.9%, and the flexural stiffness increased by 57.3%. The experimental results show that the degree of influence of various factors on the edgewise strength, flexural stiffness, and static bending strength of paperboard is in the order of particle size, cultivation time,fructose addition amount. When the particle size is 0.165～0.250 mm, the cultivation time is 30 days, and the fructose addition of 5.0%, the edgewise strength of the prepared paperboard is 14.36 N/mm, the static bending strength is 10.89 MPa, and the flexural stiffness is 33.09 mN/m. As the particle size decreases, the water absorption rate and water thickness expansion rate of the paperboard after 24 hours of water absorption decrease to 0.86% and 0.59%. The mycelium paperboard prepared by different single factors has the same thermal decomposition process, starting at around 150 ℃ and reaching its maximum at 350 ℃. This study provides a reference basis for the development and application of mycelium composite materials in the future. [ABSTRACT FROM AUTHOR]

Banana fusarium wilt has a significant adverse effect on the banana industry. It is worth noting that fusarium oxysporum f.sp. cubense 4(Foc4) is one of the most devastating and widespread pathogens of banana fusarium wilt in the world. It is a catastrophic threat to the global banana industry. At present, there is still no technology that can cure bananas infected with banana fusarium wilt. Therefore, it is extremely important for banana planting industry to detect banana fusarium wilt in time and do a good job in prevention and control. In order to realize the early accurate detection of banana fusarium wilt race 4, this study proposed a detection method of double probe paper-based sensor based on colloidal gold, which increased the use of signal enhancement probes to increase the binding amount of colloidal gold, thereby reducing the detection limit. Two kinds of colloidal gold particles with different particle sizes were combined with the corresponding detection probes and signal enhancement probes to form a double gold label probe system. The large particle size colloidal gold was used with the detection probe, while the small particle size colloidal gold was paired with the signal enhancement probe. The sample being tested was mixed with this double gold-labeled probe system. The Foc4 target sequence in the sample would be paired with the double gold-labeled probe to form a ' gold-labeled probe-target sequence-T-line probe ' complex, which continued to migrate to the water-absorbing pad. The complex was captured on the test area of the sensor. Within 10 minutes, the test paper would generate a visible target product that could be observed by the naked eye at both the test line and the quality control line areas, thus achieving qualitative detection. For quantitative detection of Foc4, the light intensity of the test strip would be further analyzed using software. The light intensity peak area was obtained and substituted into the standard curve to obtain the concentration of Foc4 detected and realize quantitative detection. Experimental results demonstrate that the detection limit of the dual-probe paper-based sensor is 0.001 nmol/L, which is 100 times of that of the traditional paper-based sensor, and the detection sensitivity is improved. The sensitivity test results demonstrated a strong linear relationship between the concentration of Foc4 and the peak area of the test line light intensity within the concentration range of 0.001-1 000.000 nmol/L. This finding suggests that the sensor can be effectively employed for quantitative detection. To assess specificity, a high concentration of non-complementary probe was utilized as the target sequence for detection and compared with the detection results of samples containing Foc4. The experiment revealed the absence of a red band at the test line when the test paper was detected by the noncomplementary sequence. Importantly, this observation indicated that the presence of the non-complementary sequence had minimal impact on the detection effect, thus affirming the sensor's excellent specificity. Finally, the paper-based sensor was used to detect Foc4 in banana leaves. The average recovery rate was 77.6%-102.3%, and the relative standard deviation was 7.4%-7.7%, indicating that it can be used for on-site detection of Foc4. The detection technology possesses the characteristics of being low-cost and easy to operate. In comparison to traditional detection methods, it enables timely, rapid and accurately determination of the presence of Foc4. Compared with the existing molecular detection technology, the detection can be completed without the need of expensive equipment or specialized personnel. This feature makes it highly suitable for widespread use. The dual-probe paper-based sensor realizes the combination of nucleic acid lateral chromatography detection technology and banana fusarium wilt detection. Its potential for widespread adoption in the early disease warning system of the banana industry is substantial. Furthermore, this technology can be combined with traditional polymerase chain reaction (PCR) and other techniques to facilitate on-site detection of Foc4. [ABSTRACT FROM AUTHOR]

A high-precision mechanized seeding can greatly contribute to the vegetable production at present. In this study, a spotting glue-paper tape precision seeder was designed for the small seed vegetables, particularly combining the seed rope and printing seeding. Three components were also divided in the seeder, including the peristaltic pump glue, ratchet spotting glue, and seed metering device. Among them, the glue was a mixture of pregelatinized starch and water. Specifically, the glue volume and viscosity were the main factors affecting the number of adhered seeds. The glue point volume depended mainly on the rotation angle of the peristaltic pump during the movement. A pretest of seed adhesion was carried out to preliminarily determine the mass ratio of pregelatinized starch to water (less than 1:5), representing the glue viscosity. Furthermore, the frequency of pump and dispensing were analyzed to clarify the relationship between the peristaltic pump and ratchet speed, where the number of ratchet teeth was determined to be 4. As such, the matching was realized between the peristaltic pump and ratchet spotting glue. The ratchet profile was also designed, according to the spotting glue motion. A theoretical analysis was made on the movement of seeds in the seed guiding tube. It was found that the aggregation effect of seeds on the paper tape relied on the length and cross-sectional angle of the seed guiding tube, as well as the rotation speed of the socket wheel. The seed guiding tube was then optimized for the optimal structural parameters. A Box_Behnken test was also carried out, where the length and cross-sectional angle of the seed guiding tube, while the rotation speed of the socket wheel was taken as the test factors, whereas, the percentage of seeds in the effective area on the paper tape was taken as the test index. The results showed that the percentage of seeds in the effective area on the paper tape was 57.23%, when the length of the seed guiding tube was 56 mm, the section angle was 35°, and the rotation speed of the seed metering wheel was 30 r/min. An adhesion test was also performed on the Shanghai Qing 605 seeds with a diameter of 1.5-2.0 mm. The test factors were then selected as the roller angle of the peristaltic pump and the mass ratio of pregelatinized starch to water. An optimal combination of parameters was achieved, including the size of the glue point, and the mass ratio of pregelatinized starch to water. Once the roller rotation angle was 10° and the mass ratio of pre-gelatinized starch to water was 1:6, the single seed rate of glue point adhesion to seeds reached 97.4%. The adaptability test was also carried out on the whole machine on the working performance of different varieties of small seed vegetables. It was found that the single seed rates of Shanghai Qing 605 and Siji Chinese cabbage seeds were greater than 95% under the working speed of 0.5-1.5 m/s, whereas, the coefficient of variation of plant spacing was less than 10%, fully meeting the requirements of precision sowing indicators that specified in the standard NY/T1143-2006. Therefore, the single seed rate and the working efficiency of the improved seeder increased by 3.53%, and 50% than before. [ABSTRACT FROM AUTHOR]

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