1. p38 MAPK is involved in human neutrophil chemotaxis induced by L-amino acid oxidase from Calloselasma rhodosthoma.
- Author
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Pontes AS, Setúbal Sda S, Nery NM, da Silva FS, da Silva SD, Fernandes CF, Stábeli RG, Soares AM, and Zuliani JP
- Subjects
- Animals, Cytokines metabolism, Dinoprostone metabolism, Humans, Leukotriene B4 metabolism, Neutrophils drug effects, Neutrophils enzymology, Neutrophils metabolism, Peroxidase metabolism, Reactive Oxygen Species metabolism, Viperidae, Chemotaxis, Leukocyte drug effects, L-Amino Acid Oxidase toxicity, Viper Venoms enzymology, p38 Mitogen-Activated Protein Kinases metabolism
- Abstract
The action of LAAO, an L-amino acid oxidase isolated from Calloselasma rhodosthoma snake venom, on isolated human neutrophil function was investigated. Cr-LAAO showed no toxicity on neutrophils. Cr-LAAO in its native form induced the neutrophil chemotaxis, suggesting that its primary structure is essential for stimulation the cell. p38 MAPK and PI3K have a role as signaling pathways of CR-LAAO induced chemotaxis. This toxin also induced the production of hydrogen peroxide and stimulated phagocytosis in neutrophils. Furthermore, Cr-LAAO was able to stimulate neutrophils to release IL-6, IL-8, MPO, LTB4 and PGE2. Together, the data showed that the Cr-LAAO triggers relevant proinflammatory events., (Copyright © 2016 Elsevier Ltd. All rights reserved.)
- Published
- 2016
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