1. Alcohol inhibits the proliferation of Neuro2a cells via promoting the asymmetric cell division through down-regulation of the expression of centrosome protein-J
- Author
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Tao Xu, Shan-Yu Zhang, Kai-Heng Zhu, Wei-Wei Zhang, Xiao-Mei Xu, Ling-Li Zhao, and Shuai Zhao
- Subjects
0301 basic medicine ,Neurogenesis ,Recombinant Fusion Proteins ,Cell ,Green Fluorescent Proteins ,Down-Regulation ,Alcohol ,Nerve Tissue Proteins ,Spindle Apparatus ,Toxicology ,Cell Line ,03 medical and health sciences ,chemistry.chemical_compound ,Mice ,Downregulation and upregulation ,Asymmetric cell division ,medicine ,Animals ,Humans ,Cell Proliferation ,Neurons ,Microscopy, Confocal ,Spindle orientation ,Ethanol ,Cell growth ,Asymmetric Cell Division ,General Medicine ,Neural stem cell ,Cell biology ,030104 developmental biology ,medicine.anatomical_structure ,chemistry ,Gene Expression Regulation ,Microscopy, Fluorescence ,Centrosome ,RNA Interference ,Microtubule-Associated Proteins - Abstract
Alcohol can decrease cell proliferation in neural cells. The proliferation of neural cells can be inhibited by the asymmetric division of neural progenitor cells. However, whether alcohol inhibits cell proliferation through inducing cell asymmetric division is not yet clear. Here, we reported that the percentage of asymmetric division was increased in alcohol-treated Neuro2a cells owing to the impaired-spindle orientation. Meanwhile, the expression of Centrosome protein-J (CPAP) which plays an important role in spindle orientation was reduced in Neuro2a cells. The overexpression of GFP-CPAP in Neuro2a cells rescued the disorder of spindle orientation and the asymmetric cell division induced by alcohol. Taken together, the results demonstrate that alcohol exposure diminished the pool of proliferative Neuro2a cells through disordering the spindle orientation and promoting the asymmetric division. And these abnormal orientation and division were due to the reduced CPAP protein level.
- Published
- 2017