1. Surface Chemistry and Microtopography of Parylene C Films Control the Morphology and Microtubule Density of Cardiac Myocytes
- Author
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Julia Gorelik, Themistoklis Prodromakis, Cesare M. Terracciano, Tatiana Trantidou, Eleanor J. Humphrey, Claire Poulet, and British Heart Foundation
- Subjects
0301 basic medicine ,Polymers ,Biomedical Engineering ,Medicine (miscellaneous) ,Bioengineering ,02 engineering and technology ,Cell Communication ,Xylenes ,Microtubules ,Article ,Contact angle ,03 medical and health sciences ,chemistry.chemical_compound ,Parylene ,Tissue engineering ,0903 Biomedical Engineering ,Microtubule ,Cell Adhesion ,Myocyte ,Animals ,Myocytes, Cardiac ,Cell adhesion ,Cells, Cultured ,Tissue Engineering ,Chemistry ,0601 Biochemistry And Cell Biology ,Adhesion ,021001 nanoscience & nanotechnology ,Rats ,030104 developmental biology ,Membrane ,Animals, Newborn ,Biophysics ,0210 nano-technology ,Hydrophobic and Hydrophilic Interactions - Abstract
Cell micropatterning has certainly proved to improve the morphological and physiological properties of cardiomyocytes in vitro; however, there is little knowledge on the single cell-scaffold interactions that influence the cells' development and differentiation in culture. In this study, we employ hydrophobic/hydrophilic micropatterned Parylene C thin films (2-10 μm) as cell microscaffolds that can control the morphology and microtubule density of neonatal rat ventricular myocytes (NRVM) by regulating their adhesion area on Parylene through a thickness-dependent hydrophobicity. Structured NRVM on thin films tend to bridge across the hydrophobic areas, demonstrating a more spread-out shape and sparser microtubule organization, while cells on thicker films adopt a cylindrical (in vivo-like) shape (contact angles at the level of the nucleus are 64.51° and 84.73°, respectively) and a significantly (p
- Published
- 2016